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The map label for this gene is aatA [H]

Identifier: 87198471

GI number: 87198471

Start: 489185

End: 490354

Strand: Direct

Name: aatA [H]

Synonym: Saro_0446

Alternate gene names: 87198471

Gene position: 489185-490354 (Clockwise)

Preceding gene: 87198470

Following gene: 87198472

Centisome position: 13.74

GC content: 68.21

Gene sequence:

>1170_bases
ATGAACCAGCTCTCCCGGGCGCTTGCGCGCATCGCTCCTTCCCGTACCACCGCTATCACCGACCGCGCGATCCAGCTTCG
CGCCGAAGGCCGCGACGTGATCTCGCTCTCGGTGGGCGAGCCTGATTTCGCCACGCCCGCGCACGTCGTCCAGGCCACCA
AGGACGCGCTCGACGCAGGCGACACCAAGTATACCGCCGTCGTGGGCACAGCCGCCCTGCGCAGCGCCGCCGCGCTGCAC
TTCAGCCGTGACCTCGGCCTGGAAGTCCCGCCCTCGCAGGTGATCGTCAGTGCCGGCGGCAAGCAGGCGATCTTCCACGC
CCTTCTCGCCACGCTCGATCCCGGCGACGAAGTACTGATCCCCAGCCCCTGGTGGGTCAGCTACCCTGAAATCGTGCGTT
TCGCCGGAGCAGAGGTCGTGGACCTGCCGACCGACGCCGCAGGCGGTTTCCGCATTACGGCCGCGCAACTCGAGGCCGCA
ATCACCCCCGCCACCCGCTGGCTGCTGCTTAACAGCCCCGGCAACCCCACTGGCGCCACCTATCCGGCGCAGGAACTGCG
CGCGCTGGGCGAGGTTCTGCGCCGTCATCCCCGCGTGCTGGTGATGAGCGACGACATCTATGCGCCCCTGCGTTACGGCG
AGGGCCGCCACGCCACGCTGGCGGTGGAGTGCCCCGATCTCGCGGATCGCATCCTGACCGTCTCCGGCGTTTCGAAAAGC
CACGCGATGACCGGTTTCCGGATCGGCGTCGCCGCCGGCCCCGCATGGCTGATCTCTGCGATGGGCCGCCTGCAATCGCA
TTCCTCGGGCAACCCTGCCTCGATAAGCCAGGCCGCTGCGGTCGCCGCGTTCGAAGGCCCGCAGGACTTCCTGCTGGACT
GGCGCGAGCGCTTCCGTGCGCGCCGGGACATGGTCTGCGCGCGCGTTAACGCGATCCCCGGCCTGTCCACGCCTGTTCCC
GATGGCGCCTTCTACTGTATGGTCGATGCTGCGCCGTTGATGGCGCGCTTCGGCGATGACGAAGCGCTCTGCCTCCATCT
GTTGGAAAGCGGCGTGGCCGTGGTGCCGGCATCCGCGTTCGGCGGAAGGGACGGCTTCCGCATCAGCTTCGCGGCGGACG
AGGCGAAACTCGAAGAAGCGCTGCGGCGTATAGAAAAGGCCGTTGCATGA

Upstream 100 bases:

>100_bases
GTCGAGGCGGACCTGACGCTCGATGACCTTGCCGGCGGCTTCCTCGTCGGCAACGCGCTGCGCGGCCTCATCCCGGCTCG
CCTGCTTGGCGCCTGACATC

Downstream 100 bases:

>100_bases
TCGATTTCTCCATCCTGTTCGAAGACGGCGAGGCGCTGGTCATCGACAAGCCGGCTGGCCTCCCGCTCGATCGTCCTCGC
GCCGGTGGCGACAGCCTCGA

Product: aminotransferase

Products: NA

Alternate protein names: AspAT; Transaminase A [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNQLSRALARIAPSRTTAITDRAIQLRAEGRDVISLSVGEPDFATPAHVVQATKDALDAGDTKYTAVVGTAALRSAAALH
FSRDLGLEVPPSQVIVSAGGKQAIFHALLATLDPGDEVLIPSPWWVSYPEIVRFAGAEVVDLPTDAAGGFRITAAQLEAA
ITPATRWLLLNSPGNPTGATYPAQELRALGEVLRRHPRVLVMSDDIYAPLRYGEGRHATLAVECPDLADRILTVSGVSKS
HAMTGFRIGVAAGPAWLISAMGRLQSHSSGNPASISQAAAVAAFEGPQDFLLDWRERFRARRDMVCARVNAIPGLSTPVP
DGAFYCMVDAAPLMARFGDDEALCLHLLESGVAVVPASAFGGRDGFRISFAADEAKLEEALRRIEKAVA

Sequences:

>Translated_389_residues
MNQLSRALARIAPSRTTAITDRAIQLRAEGRDVISLSVGEPDFATPAHVVQATKDALDAGDTKYTAVVGTAALRSAAALH
FSRDLGLEVPPSQVIVSAGGKQAIFHALLATLDPGDEVLIPSPWWVSYPEIVRFAGAEVVDLPTDAAGGFRITAAQLEAA
ITPATRWLLLNSPGNPTGATYPAQELRALGEVLRRHPRVLVMSDDIYAPLRYGEGRHATLAVECPDLADRILTVSGVSKS
HAMTGFRIGVAAGPAWLISAMGRLQSHSSGNPASISQAAAVAAFEGPQDFLLDWRERFRARRDMVCARVNAIPGLSTPVP
DGAFYCMVDAAPLMARFGDDEALCLHLLESGVAVVPASAFGGRDGFRISFAADEAKLEEALRRIEKAVA
>Mature_389_residues
MNQLSRALARIAPSRTTAITDRAIQLRAEGRDVISLSVGEPDFATPAHVVQATKDALDAGDTKYTAVVGTAALRSAAALH
FSRDLGLEVPPSQVIVSAGGKQAIFHALLATLDPGDEVLIPSPWWVSYPEIVRFAGAEVVDLPTDAAGGFRITAAQLEAA
ITPATRWLLLNSPGNPTGATYPAQELRALGEVLRRHPRVLVMSDDIYAPLRYGEGRHATLAVECPDLADRILTVSGVSKS
HAMTGFRIGVAAGPAWLISAMGRLQSHSSGNPASISQAAAVAAFEGPQDFLLDWRERFRARRDMVCARVNAIPGLSTPVP
DGAFYCMVDAAPLMARFGDDEALCLHLLESGVAVVPASAFGGRDGFRISFAADEAKLEEALRRIEKAVA

Specific function: Required for symbiotic nitrogen fixation [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=397, Percent_Identity=25.9445843828715, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI169881279, Length=397, Percent_Identity=25.9445843828715, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI56713256, Length=359, Percent_Identity=23.6768802228412, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI56713254, Length=359, Percent_Identity=23.6768802228412, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4507369, Length=391, Percent_Identity=26.8542199488491, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI169881281, Length=383, Percent_Identity=21.1488250652742, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788722, Length=388, Percent_Identity=30.1546391752577, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786816, Length=366, Percent_Identity=26.5027322404372, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788627, Length=364, Percent_Identity=27.1978021978022, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790797, Length=370, Percent_Identity=25.4054054054054, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787710, Length=339, Percent_Identity=26.5486725663717, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787909, Length=374, Percent_Identity=25.668449197861, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71994472, Length=399, Percent_Identity=25.31328320802, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994476, Length=399, Percent_Identity=25.31328320802, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17567663, Length=377, Percent_Identity=25.9946949602122, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17567369, Length=360, Percent_Identity=23.6111111111111, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6322401, Length=398, Percent_Identity=24.3718592964824, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28573069, Length=388, Percent_Identity=24.4845360824742, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646114, Length=388, Percent_Identity=24.4845360824742, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28573067, Length=388, Percent_Identity=24.4845360824742, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28573065, Length=388, Percent_Identity=24.4845360824742, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI18859735, Length=380, Percent_Identity=24.7368421052632, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.1 [H]

Molecular weight: Translated: 41263; Mature: 41263

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLSRALARIAPSRTTAITDRAIQLRAEGRDVISLSVGEPDFATPAHVVQATKDALDAG
CCHHHHHHHHHCCCCHHHHHHHEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCC
DTKYTAVVGTAALRSAAALHFSRDLGLEVPPSQVIVSAGGKQAIFHALLATLDPGDEVLI
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEE
PSPWWVSYPEIVRFAGAEVVDLPTDAAGGFRITAAQLEAAITPATRWLLLNSPGNPTGAT
CCCCCCCCHHHHHHCCCEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCCCC
YPAQELRALGEVLRRHPRVLVMSDDIYAPLRYGEGRHATLAVECPDLADRILTVSGVSKS
CCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCEEEEEECCHHHHHEEEEECCCHH
HAMTGFRIGVAAGPAWLISAMGRLQSHSSGNPASISQAAAVAAFEGPQDFLLDWRERFRA
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
RRDMVCARVNAIPGLSTPVPDGAFYCMVDAAPLMARFGDDEALCLHLLESGVAVVPASAF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHCCCEEEECHHC
GGRDGFRISFAADEAKLEEALRRIEKAVA
CCCCCEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQLSRALARIAPSRTTAITDRAIQLRAEGRDVISLSVGEPDFATPAHVVQATKDALDAG
CCHHHHHHHHHCCCCHHHHHHHEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCC
DTKYTAVVGTAALRSAAALHFSRDLGLEVPPSQVIVSAGGKQAIFHALLATLDPGDEVLI
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEE
PSPWWVSYPEIVRFAGAEVVDLPTDAAGGFRITAAQLEAAITPATRWLLLNSPGNPTGAT
CCCCCCCCHHHHHHCCCEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCCCC
YPAQELRALGEVLRRHPRVLVMSDDIYAPLRYGEGRHATLAVECPDLADRILTVSGVSKS
CCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCEEEEEECCHHHHHEEEEECCCHH
HAMTGFRIGVAAGPAWLISAMGRLQSHSSGNPASISQAAAVAAFEGPQDFLLDWRERFRA
HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
RRDMVCARVNAIPGLSTPVPDGAFYCMVDAAPLMARFGDDEALCLHLLESGVAVVPASAF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHCCCEEEECHHC
GGRDGFRISFAADEAKLEEALRRIEKAVA
CCCCCEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2019560; 8096210; 11481430 [H]