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The map label for this gene is ruvC

Identifier: 87198446

GI number: 87198446

Start: 458696

End: 459172

Strand: Direct

Name: ruvC

Synonym: Saro_0421

Alternate gene names: 87198446

Gene position: 458696-459172 (Clockwise)

Preceding gene: 87198445

Following gene: 87198447

Centisome position: 12.88

GC content: 65.62

Gene sequence:

>477_bases
ATGATCATCCTCGGCATCGACCCCGGCCTCACCGTCACCGGCTGGGGCGTCGTCGCCAAGTCGGGCAGCCGGTTGAGCCA
CGTGGCCAACGGCCAGATCCGCACTGACAACAAGGCCACGATGGCCGAGCGCCTCGTCGAACTCGATTCCGCGCTCGCCG
CCGTGATCGACATCTACAAGCCCGATACCGGCGCGGTCGAAGAGGTCTTCGTCAACGTCAATCCGCAGTCCACGCTGAAG
CTGGGGCAGGCACGCGGCGTGGCGATGGTCGCCGTGGCGCGCTCCGGCATTCCGGTGGCGGAATATCCGACCAAGGTCAT
CAAGAAGGCGCTGGTCGGGACGGGCGGCGCCGAAAAGCAGCAGATCCAGCACATGCTCAAGATCCTGCTCCCCGGCGTTC
AACTGGCGGGCGAGGATGCCTCCGACGCATTGGCCGTGGCGATTACCCACGCCAACATGCTGGGCAGCCATCGATAG

Upstream 100 bases:

>100_bases
GTGCCGTTCTGGATATCACTTATCGCCGGCTGCTTGCTGACAGTCCTGCTCTATCTGGCAATGATGCAACTGGCCCCGCG
CATCGGACTGAAACTGTAAG

Downstream 100 bases:

>100_bases
CGGAACCAGCGGCAATCGGCGCCGCATTGCGGAAGCCGGAAGCCTGCAAGTCCACCCGCTTGCCCCTGCCTGCGTTCCCT
GCCATAGGAACAAACCATGA

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIILGIDPGLTVTGWGVVAKSGSRLSHVANGQIRTDNKATMAERLVELDSALAAVIDIYKPDTGAVEEVFVNVNPQSTLK
LGQARGVAMVAVARSGIPVAEYPTKVIKKALVGTGGAEKQQIQHMLKILLPGVQLAGEDASDALAVAITHANMLGSHR

Sequences:

>Translated_158_residues
MIILGIDPGLTVTGWGVVAKSGSRLSHVANGQIRTDNKATMAERLVELDSALAAVIDIYKPDTGAVEEVFVNVNPQSTLK
LGQARGVAMVAVARSGIPVAEYPTKVIKKALVGTGGAEKQQIQHMLKILLPGVQLAGEDASDALAVAITHANMLGSHR
>Mature_158_residues
MIILGIDPGLTVTGWGVVAKSGSRLSHVANGQIRTDNKATMAERLVELDSALAAVIDIYKPDTGAVEEVFVNVNPQSTLK
LGQARGVAMVAVARSGIPVAEYPTKVIKKALVGTGGAEKQQIQHMLKILLPGVQLAGEDASDALAVAITHANMLGSHR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=129, Percent_Identity=46.5116279069767, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_NOVAD (Q2GBA4)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495703.1
- ProteinModelPortal:   Q2GBA4
- SMR:   Q2GBA4
- STRING:   Q2GBA4
- GeneID:   3917567
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0421
- NMPDR:   fig|48935.1.peg.3723
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q2GBA4
- BioCyc:   NARO279238:SARO_0421-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 16471; Mature: 16471

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGIDPGLTVTGWGVVAKSGSRLSHVANGQIRTDNKATMAERLVELDSALAAVIDIYK
CEEEECCCCCEEEECEEEECCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PDTGAVEEVFVNVNPQSTLKLGQARGVAMVAVARSGIPVAEYPTKVIKKALVGTGGAEKQ
CCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHH
QIQHMLKILLPGVQLAGEDASDALAVAITHANMLGSHR
HHHHHHHHHHCCCEECCCCCCCEEEEEEEHHHHHCCCC
>Mature Secondary Structure
MIILGIDPGLTVTGWGVVAKSGSRLSHVANGQIRTDNKATMAERLVELDSALAAVIDIYK
CEEEECCCCCEEEECEEEECCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCC
PDTGAVEEVFVNVNPQSTLKLGQARGVAMVAVARSGIPVAEYPTKVIKKALVGTGGAEKQ
CCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHH
QIQHMLKILLPGVQLAGEDASDALAVAITHANMLGSHR
HHHHHHHHHHCCCEECCCCCCCEEEEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA