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The map label for this gene is ruvA

Identifier: 87198447

GI number: 87198447

Start: 459269

End: 459883

Strand: Direct

Name: ruvA

Synonym: Saro_0422

Alternate gene names: 87198447

Gene position: 459269-459883 (Clockwise)

Preceding gene: 87198446

Following gene: 87198448

Centisome position: 12.9

GC content: 67.8

Gene sequence:

>615_bases
ATGATCGCCAAGCTCACCGGCATTCTTGACGACACCGGGCCCGATTGGGCCGTGATCGACGTGAACGGCGTCGGCTACCT
CGTCCATTGCTCGGCCAAGACGCTCACCCATCTCGGCCTCCGGGGTGACAAGGTCGTCGTCCACACCGAAATGCAGGTGA
GCGAGACCGACCAGCGCCTGATCGGCTTTACCAGCGCGGGCGAACGCGCGTGGTTCCGGCTGCTGACGGCGGTGCAGGGC
GTCGGCAGCAAGGTCGCTCTTGCAATCCTTTCGGCACTGTCGGTCGAGGAACTCCAGCGCGCCTGTGCCCACGGCGACAG
CGCGATGGTGGCGCGCGCCAATGGCGTCGGCCCCAAGCTCGCCAGCCGCATCGTCAACGAACTGAAGGACAAGGCCGGCG
GTCTTGCGGGCTATGCCTCGCCGGTCGGCCCGGGCGGCGAGGCCTTCGTCGCGCCCCCTGGAAATGCCAGCGCGGATGCG
GTCTCGGCCTTGCAGAACCTCGGTTTCAAACCCGCCGTCGCCAGCAGCGCCGTCGCCGCAGCAGTCAAGGAACTGGGCGA
GGATGCGGGGTTGAACGACCTGGTGCGCGTGGCGCTGAAGCGGGCGGCGGGATAA

Upstream 100 bases:

>100_bases
ATAGCGGAACCAGCGGCAATCGGCGCCGCATTGCGGAAGCCGGAAGCCTGCAAGTCCACCCGCTTGCCCCTGCCTGCGTT
CCCTGCCATAGGAACAAACC

Downstream 100 bases:

>100_bases
CGAATGACCGACAACCCGCTCCTTTCCTCCGCCTCGCAGATCGAGGATCAGGACGCCGCGCTGCGGCCCAAGACGCTGGC
CGAGTTCGTCGGCCAGGCCG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIAKLTGILDDTGPDWAVIDVNGVGYLVHCSAKTLTHLGLRGDKVVVHTEMQVSETDQRLIGFTSAGERAWFRLLTAVQG
VGSKVALAILSALSVEELQRACAHGDSAMVARANGVGPKLASRIVNELKDKAGGLAGYASPVGPGGEAFVAPPGNASADA
VSALQNLGFKPAVASSAVAAAVKELGEDAGLNDLVRVALKRAAG

Sequences:

>Translated_204_residues
MIAKLTGILDDTGPDWAVIDVNGVGYLVHCSAKTLTHLGLRGDKVVVHTEMQVSETDQRLIGFTSAGERAWFRLLTAVQG
VGSKVALAILSALSVEELQRACAHGDSAMVARANGVGPKLASRIVNELKDKAGGLAGYASPVGPGGEAFVAPPGNASADA
VSALQNLGFKPAVASSAVAAAVKELGEDAGLNDLVRVALKRAAG
>Mature_204_residues
MIAKLTGILDDTGPDWAVIDVNGVGYLVHCSAKTLTHLGLRGDKVVVHTEMQVSETDQRLIGFTSAGERAWFRLLTAVQG
VGSKVALAILSALSVEELQRACAHGDSAMVARANGVGPKLASRIVNELKDKAGGLAGYASPVGPGGEAFVAPPGNASADA
VSALQNLGFKPAVASSAVAAAVKELGEDAGLNDLVRVALKRAAG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=212, Percent_Identity=34.4339622641509, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_NOVAD (Q2GBA3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495704.1
- ProteinModelPortal:   Q2GBA3
- SMR:   Q2GBA3
- STRING:   Q2GBA3
- GeneID:   3917568
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0422
- NMPDR:   fig|48935.1.peg.3722
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   YKTAMSI
- PhylomeDB:   Q2GBA3
- ProtClustDB:   PRK00116
- BioCyc:   NARO279238:SARO_0422-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20750; Mature: 20750

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKLTGILDDTGPDWAVIDVNGVGYLVHCSAKTLTHLGLRGDKVVVHTEMQVSETDQRL
CCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHCCCCCEEEEEECCCCCCCHHHH
IGFTSAGERAWFRLLTAVQGVGSKVALAILSALSVEELQRACAHGDSAMVARANGVGPKL
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCHHH
ASRIVNELKDKAGGLAGYASPVGPGGEAFVAPPGNASADAVSALQNLGFKPAVASSAVAA
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHH
AVKELGEDAGLNDLVRVALKRAAG
HHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAKLTGILDDTGPDWAVIDVNGVGYLVHCSAKTLTHLGLRGDKVVVHTEMQVSETDQRL
CCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHHHCCCCCEEEEEECCCCCCCHHHH
IGFTSAGERAWFRLLTAVQGVGSKVALAILSALSVEELQRACAHGDSAMVARANGVGPKL
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCHHH
ASRIVNELKDKAGGLAGYASPVGPGGEAFVAPPGNASADAVSALQNLGFKPAVASSAVAA
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHH
AVKELGEDAGLNDLVRVALKRAAG
HHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA