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The map label for this gene is trmB

Identifier: 87198330

GI number: 87198330

Start: 330670

End: 331386

Strand: Direct

Name: trmB

Synonym: Saro_0305

Alternate gene names: 87198330

Gene position: 330670-331386 (Clockwise)

Preceding gene: 87198328

Following gene: 87198331

Centisome position: 9.28

GC content: 67.22

Gene sequence:

>717_bases
ATGACAGCCTACAAATCCGGCGATCCGACGACGATCAACCGCCTCTATGGCCGCGCCAAGGGCAAGCCCCTGCGCCAGGG
CCAGCAGGCGCTGGTGGACGAACTGCTCCCGCAGATCTCGATGCCCGCCGAGGGGCCGATCACGGCGGAGGCGCTGTTCG
GCGAGCCGCGCCCGCTGCACTTCGAGATCGGCTTCGGCGGCGGCGAGCACATGGCGTTCCGCGCCGACATGCTGCCCGAC
CACGGCTTCATCGGGGCAGAGCCGTTCCTCAACGGCGTCGCGCAGGCTCTCACCCACGTTTCTGGGGACAACGGCCAGCA
TCCGCCGATCCCCAATGTCCGCATCCACCATGGCGATGCGCTTGAAGTGCTGCGACGCATTCCGGATGGCTCGCTGTCGT
TTCTCTACCTCCTCCACCCGGACCCCTGGCCCAAGGCGCGCCATGCCAAGCGCCGCATGATGAACGACGGCCCGGTCGAT
CTCTTCGCCGCCAAGCTTCGTCCCGGCGGGGAGTTTCGCTTCGGCACCGATCACGCGGTCTACCTGCGCCACGCGCTGAT
GGTGATGCGCCGGCACAAGCACCAGTTCGAATGGCTGGCGAAGGACGCGAGGGACTTCCAGGTCCGCCCCGGCGGCTGGC
CCGAAACCCGCTACGAACACAAGGCGCGCACCGTCTATGGCCACGAGGTGTGGTACTTCCGTTTCAGGCGACGGTGA

Upstream 100 bases:

>100_bases
CACCACAGCAGGCCCGCACCGTCCATCCAATTTTCTTGTACCGGGGCGGCAGGACTGATCGTATTGACCCCTGCGGACCG
CCCTGCCAAACGCCCCCGCC

Downstream 100 bases:

>100_bases
TCTGCGGTTTTTTGGCTGAAAACCTCAGGAAATGGGCCATTTCGCCTACGCCAGCCTAACCGGCGATTTCCTAGTTTTTC
CGCTTGCGTGATTGCCAGGT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTAYKSGDPTTINRLYGRAKGKPLRQGQQALVDELLPQISMPAEGPITAEALFGEPRPLHFEIGFGGGEHMAFRADMLPD
HGFIGAEPFLNGVAQALTHVSGDNGQHPPIPNVRIHHGDALEVLRRIPDGSLSFLYLLHPDPWPKARHAKRRMMNDGPVD
LFAAKLRPGGEFRFGTDHAVYLRHALMVMRRHKHQFEWLAKDARDFQVRPGGWPETRYEHKARTVYGHEVWYFRFRRR

Sequences:

>Translated_238_residues
MTAYKSGDPTTINRLYGRAKGKPLRQGQQALVDELLPQISMPAEGPITAEALFGEPRPLHFEIGFGGGEHMAFRADMLPD
HGFIGAEPFLNGVAQALTHVSGDNGQHPPIPNVRIHHGDALEVLRRIPDGSLSFLYLLHPDPWPKARHAKRRMMNDGPVD
LFAAKLRPGGEFRFGTDHAVYLRHALMVMRRHKHQFEWLAKDARDFQVRPGGWPETRYEHKARTVYGHEVWYFRFRRR
>Mature_237_residues
TAYKSGDPTTINRLYGRAKGKPLRQGQQALVDELLPQISMPAEGPITAEALFGEPRPLHFEIGFGGGEHMAFRADMLPDH
GFIGAEPFLNGVAQALTHVSGDNGQHPPIPNVRIHHGDALEVLRRIPDGSLSFLYLLHPDPWPKARHAKRRMMNDGPVDL
FAAKLRPGGEFRFGTDHAVYLRHALMVMRRHKHQFEWLAKDARDFQVRPGGWPETRYEHKARTVYGHEVWYFRFRRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=235, Percent_Identity=33.6170212765957, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NOVAD (Q2GBM0)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495587.1
- ProteinModelPortal:   Q2GBM0
- SMR:   Q2GBM0
- STRING:   Q2GBM0
- GeneID:   3916242
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0305
- NMPDR:   fig|48935.1.peg.4007
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- PhylomeDB:   Q2GBM0
- ProtClustDB:   PRK00121
- BioCyc:   NARO279238:SARO_0305-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27165; Mature: 27034

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: BINDING 62-62 BINDING 87-87 BINDING 119-119 BINDING 141-141 BINDING 145-145 BINDING 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYKSGDPTTINRLYGRAKGKPLRQGQQALVDELLPQISMPAEGPITAEALFGEPRPLH
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCEE
FEIGFGGGEHMAFRADMLPDHGFIGAEPFLNGVAQALTHVSGDNGQHPPIPNVRIHHGDA
EEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
LEVLRRIPDGSLSFLYLLHPDPWPKARHAKRRMMNDGPVDLFAAKLRPGGEFRFGTDHAV
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHEEEECCCCCCCEECCCCHHH
YLRHALMVMRRHKHQFEWLAKDARDFQVRPGGWPETRYEHKARTVYGHEVWYFRFRRR
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHEEECCEEEEEEEECC
>Mature Secondary Structure 
TAYKSGDPTTINRLYGRAKGKPLRQGQQALVDELLPQISMPAEGPITAEALFGEPRPLH
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCEE
FEIGFGGGEHMAFRADMLPDHGFIGAEPFLNGVAQALTHVSGDNGQHPPIPNVRIHHGDA
EEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
LEVLRRIPDGSLSFLYLLHPDPWPKARHAKRRMMNDGPVDLFAAKLRPGGEFRFGTDHAV
HHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHEEEECCCCCCCEECCCCHHH
YLRHALMVMRRHKHQFEWLAKDARDFQVRPGGWPETRYEHKARTVYGHEVWYFRFRRR
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA