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The map label for this gene is phnI [H]

Identifier: 86751199

GI number: 86751199

Start: 4661320

End: 4662426

Strand: Direct

Name: phnI [H]

Synonym: RPB_4091

Alternate gene names: 86751199

Gene position: 4661320-4662426 (Clockwise)

Preceding gene: 86751198

Following gene: 86751200

Centisome position: 87.43

GC content: 67.93

Gene sequence:

>1107_bases
ATGTATGTCGCCGTCAAGGGTGGCGAGCGCGCCATCGACAACGCCCACCGGCTGCTGGCGCACGAGCGCCGCGGCGACCG
CGCCGTGCCTGAACTGACACTGGCGCAGATTTCCGAACAGCTCGCGCTCGGCGTCGACCGGGTGATGGTCGAGGGCTCGC
TGTATGACCGCGAGTTGGCGGCACTCGCCATCAAGCAGGCCCGCGGCGATCTGATCGAGGCGATCTTCCTGCTGCGCGCA
TTCCGCGCCACGCTGCCCCGGTTCGGCGCCACCGAACCGCTCGACACCGGCGCGATGGCGGTGCGGCGGCGGGTGTCCTC
GACCTTCAAGGATATTCCCGGCGGGCAGATTCTCGGACCGACCTTCGACTACACCCATCGGCTGCTCGATCCGCAACTCG
CCGCAGGCGGCGAGCCGGATCAGCCGGTCAATGCGGAGGTGTCGAACGAAGCGATGCCGCGCGTCACCGATATCCTCGGC
CGCGACGGACTGATCGAGCCGTCGCCCGCGCCGGCCCCCGATGCGCCGGTCGGCGACCTCACCCGCGAGCCGCTGAACTT
TCCGGCGGATCGCGATCTGCGGCTGCAAAATCTGGCGCGCGGCGACGAGGGCTTCCTGCTCGCGCTCGGCTACTCGACGC
AGCGTGGCTATGGCCGCTCGCATCCGTTCGCCGGCGAAATCCGCTTCGGCGAAGTCGAACTGGAGTTCGTCGCCGAGGAC
GTCGGCTTCGCGGTGCCGCTCGGCAGCATCGCGCTGACAGAATGCCAGATGGTCAATCAGTTCAAGGGTTCGGCCACAGA
GCCGCCCTGCTTCACCCGCGGCTACGGCCTCGCCTTCGGGCAGAGCGAGCGCAAGACCATGGCGATGGCGCTGGTCGATC
GTGCGCTCCGCGCCAAGGAACTCGGCGAGGAGGCGCTGGCGCCGGCGCAGGACGAGGAATTCGTGCTGTCGCATTCCGAC
AATGTGCAGGCGACCGGCTTCGTCGAACATCTGAAGCTGCCGCATTATGTCGACTTCCAGTCCGAGCTCGGACTGATCCG
CCGGCTGCGGCAGGATTTTGCCGATGCCGCCGAGCAAGCGGATGCGTTGCGGGAGGCCGCGGAATGA

Upstream 100 bases:

>100_bases
ACGTCCTGTTTCCGCGCGGCATCGATCTGATCCTGGTCTCGGGCCCTGCGATGATCGCGATCCCGCGCACCACGCGGCTC
GTCGCCAAGGGAGGCAACTG

Downstream 100 bases:

>100_bases
ACGCGCCACAATACAACTTCGCCTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAGGCGATCGCG
ATCCCCGGCTATCAGGTGCC

Product: phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MYVAVKGGERAIDNAHRLLAHERRGDRAVPELTLAQISEQLALGVDRVMVEGSLYDRELAALAIKQARGDLIEAIFLLRA
FRATLPRFGATEPLDTGAMAVRRRVSSTFKDIPGGQILGPTFDYTHRLLDPQLAAGGEPDQPVNAEVSNEAMPRVTDILG
RDGLIEPSPAPAPDAPVGDLTREPLNFPADRDLRLQNLARGDEGFLLALGYSTQRGYGRSHPFAGEIRFGEVELEFVAED
VGFAVPLGSIALTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMAMALVDRALRAKELGEEALAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLIRRLRQDFADAAEQADALREAAE

Sequences:

>Translated_368_residues
MYVAVKGGERAIDNAHRLLAHERRGDRAVPELTLAQISEQLALGVDRVMVEGSLYDRELAALAIKQARGDLIEAIFLLRA
FRATLPRFGATEPLDTGAMAVRRRVSSTFKDIPGGQILGPTFDYTHRLLDPQLAAGGEPDQPVNAEVSNEAMPRVTDILG
RDGLIEPSPAPAPDAPVGDLTREPLNFPADRDLRLQNLARGDEGFLLALGYSTQRGYGRSHPFAGEIRFGEVELEFVAED
VGFAVPLGSIALTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMAMALVDRALRAKELGEEALAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLIRRLRQDFADAAEQADALREAAE
>Mature_368_residues
MYVAVKGGERAIDNAHRLLAHERRGDRAVPELTLAQISEQLALGVDRVMVEGSLYDRELAALAIKQARGDLIEAIFLLRA
FRATLPRFGATEPLDTGAMAVRRRVSSTFKDIPGGQILGPTFDYTHRLLDPQLAAGGEPDQPVNAEVSNEAMPRVTDILG
RDGLIEPSPAPAPDAPVGDLTREPLNFPADRDLRLQNLARGDEGFLLALGYSTQRGYGRSHPFAGEIRFGEVELEFVAED
VGFAVPLGSIALTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMAMALVDRALRAKELGEEALAPAQDEEFVLSHSD
NVQATGFVEHLKLPHYVDFQSELGLIRRLRQDFADAAEQADALREAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=354, Percent_Identity=61.2994350282486, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 40162; Mature: 40162

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGERAIDNAHRLLAHERRGDRAVPELTLAQISEQLALGVDRVMVEGSLYDRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALAIKQARGDLIEAIFLLRAFRATLPRFGATEPLDTGAMAVRRRVSSTFKDIPGGQILGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLDPQLAAGGEPDQPVNAEVSNEAMPRVTDILGRDGLIEPSPAPAPDAPVGDL
CHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TREPLNFPADRDLRLQNLARGDEGFLLALGYSTQRGYGRSHPFAGEIRFGEVELEFVAED
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCEEEEEHHHH
VGFAVPLGSIALTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMAMALVDRALRAKE
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGEEALAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQSELGLIRRLRQDFADAAEQA
HHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DALREAAE
HHHHHHCC
>Mature Secondary Structure
MYVAVKGGERAIDNAHRLLAHERRGDRAVPELTLAQISEQLALGVDRVMVEGSLYDRELA
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALAIKQARGDLIEAIFLLRAFRATLPRFGATEPLDTGAMAVRRRVSSTFKDIPGGQILGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
TFDYTHRLLDPQLAAGGEPDQPVNAEVSNEAMPRVTDILGRDGLIEPSPAPAPDAPVGDL
CHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TREPLNFPADRDLRLQNLARGDEGFLLALGYSTQRGYGRSHPFAGEIRFGEVELEFVAED
CCCCCCCCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCEEEEEHHHH
VGFAVPLGSIALTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMAMALVDRALRAKE
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGEEALAPAQDEEFVLSHSDNVQATGFVEHLKLPHYVDFQSELGLIRRLRQDFADAAEQA
HHHHHCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DALREAAE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]