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The map label for this gene is phnJ [H]

Identifier: 86751200

GI number: 86751200

Start: 4662423

End: 4663304

Strand: Direct

Name: phnJ [H]

Synonym: RPB_4092

Alternate gene names: 86751200

Gene position: 4662423-4663304 (Clockwise)

Preceding gene: 86751199

Following gene: 86751201

Centisome position: 87.45

GC content: 63.72

Gene sequence:

>882_bases
ATGAACGCGCCACAATACAACTTCGCCTATCTCGACGAGCAGACCAAGCGGATGATCCGCCGCGCGATCCTGAAGGCGAT
CGCGATCCCCGGCTATCAGGTGCCGTTCGCCAGCCGCGAGATGCCGATGCCGTATGGCTGGGGCACTGGCGGCGTGCAGG
TCACCGCAGCGATCCTCGGCCCGGACGACGTGCTGAAGGTGATCGATCAGGGCAGCGACGACACCACCAATGCGATCTCG
ATCCGGAAATTCTTCGCCAAGACCGCCGGCGTTGCGACCACCACCAGCACGACGGATGCCAGCGTGATCCAGACACGGCA
CCGGATTCCGGAAGCGCCGCTGCACGAGGGCCAGGTGCTGGTGTATCAGGTGCCGATCCCGGAGCCGCTGCGCTTTCTCG
AGCCGCGCGAGACCGAGACGCGGCGGATGCACGCGCTCGCCGAATACGGGCTGATGCACGTCAAGCTGTACGAGGACATC
GCCAAATTCGGCCACATCGCGACGTCCTACGCCTATCCGGTGAAGGTCAATTCGCGCTACGTGATGGACCCGTCGCCGAC
GCCGAAATTCGACAATCCGAAGATGGACGATTGCCCGGCGCTGCAATTGTTCGGCGCCGGCCGCGAAAAGCGGATCTATG
CGATCCCGCCCTATACGCACGTGGTGTCGCTGGACTTCCAGGACCATCCGTTCACGCGCTATCGCCAGGACGCGCCCTGC
GCGCTGTGCGGATCGGAGGAGTCGTATCTAGACGAGATCGTCACCGACGATCGCGGCGGACGGATGTTCGTCTGCTCCGA
CACCGATTATTGCGAAACACGTCAGGTGCAAGGCCATCGCGGCAGCGAAAGCGCGCCGCATCAGGAGCGCGCAGATGCCT
GA

Upstream 100 bases:

>100_bases
ATTATGTCGACTTCCAGTCCGAGCTCGGACTGATCCGCCGGCTGCGGCAGGATTTTGCCGATGCCGCCGAGCAAGCGGAT
GCGTTGCGGGAGGCCGCGGA

Downstream 100 bases:

>100_bases
TCGCACCTCATCCTCTCGGTTCAATGAACCGCTGCTGATCGCCGAAGGCCTGGCCAAATCCTACGGCCGACTGCAGGCGT
GCCGCGATGTCTCGTTCACG

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNAPQYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTTDASVIQTRHRIPEAPLHEGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
AKFGHIATSYAYPVKVNSRYVMDPSPTPKFDNPKMDDCPALQLFGAGREKRIYAIPPYTHVVSLDFQDHPFTRYRQDAPC
ALCGSEESYLDEIVTDDRGGRMFVCSDTDYCETRQVQGHRGSESAPHQERADA

Sequences:

>Translated_293_residues
MNAPQYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTTDASVIQTRHRIPEAPLHEGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
AKFGHIATSYAYPVKVNSRYVMDPSPTPKFDNPKMDDCPALQLFGAGREKRIYAIPPYTHVVSLDFQDHPFTRYRQDAPC
ALCGSEESYLDEIVTDDRGGRMFVCSDTDYCETRQVQGHRGSESAPHQERADA
>Mature_293_residues
MNAPQYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAIS
IRKFFAKTAGVATTTSTTDASVIQTRHRIPEAPLHEGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
AKFGHIATSYAYPVKVNSRYVMDPSPTPKFDNPKMDDCPALQLFGAGREKRIYAIPPYTHVVSLDFQDHPFTRYRQDAPC
ALCGSEESYLDEIVTDDRGGRMFVCSDTDYCETRQVQGHRGSESAPHQERADA

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=270, Percent_Identity=77.7777777777778, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32895; Mature: 32895

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILG
CCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDVLKVIDQGSDDTTNAISIRKFFAKTAGVATTTSTTDASVIQTRHRIPEAPLHEGQVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCCCCEE
VYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIAKFGHIATSYAYPVKVNSRY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCEEEEEEECCEE
VMDPSPTPKFDNPKMDDCPALQLFGAGREKRIYAIPPYTHVVSLDFQDHPFTRYRQDAPC
EECCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCE
ALCGSEESYLDEIVTDDRGGRMFVCSDTDYCETRQVQGHRGSESAPHQERADA
EEECCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNAPQYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILG
CCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
PDDVLKVIDQGSDDTTNAISIRKFFAKTAGVATTTSTTDASVIQTRHRIPEAPLHEGQVL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCCCCEE
VYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIAKFGHIATSYAYPVKVNSRY
EEECCCCCHHHHCCCCCHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHCEEEEEEECCEE
VMDPSPTPKFDNPKMDDCPALQLFGAGREKRIYAIPPYTHVVSLDFQDHPFTRYRQDAPC
EECCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCE
ALCGSEESYLDEIVTDDRGGRMFVCSDTDYCETRQVQGHRGSESAPHQERADA
EEECCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]