Click here to switch to the map view.

The map label for this gene is linC [H]

Identifier: 86751047

GI number: 86751047

Start: 4495440

End: 4496240

Strand: Reverse

Name: linC [H]

Synonym: RPB_3939

Alternate gene names: 86751047

Gene position: 4496240-4495440 (Counterclockwise)

Preceding gene: 86751048

Following gene: 86751041

Centisome position: 84.33

GC content: 65.92

Gene sequence:

>801_bases
ATGCAGGTTGCGGGCAAGGTCGTCGTCGTCACCGGCGGCGGCAATGGAATCGGTCAGGCGATGTGCGAGGCCTTTGCCAA
GGCCGGCGCCGCCAAGGTCGTGGTCGCCGATCTCGATGAGGCCCGCGCCGAGGCGGTGGCCGCCGCGATCGGCGGCGCGG
CGTTCAAATGCGACGTCGCGAAAGAATCCGACATCAAGCACGTGATCGACGAGACCGAGCGGCGCTTCGGCCCGATCGCG
CTGTTCTGCTCCAATGCGGGGATCGGCGGCGGCTTCGATCCGCTGTCGGAGAATGCCGGCGGCGCGTCCGACGAGCCGTT
CATGAACAGCTGGATGATCCACGTGATGGCGCATGTCTATGCCGCACGGCATCTGGTGCCGCTCTACAGAGCGCGCGGCG
GCGGCTATTTCCTCAACACGATTTCGGCGGCGGGCCTGTTGTCGCAGGTCGGCAGCCCGGCCTATTCGGCGACCAAGCAC
GGCGCGGTGGGCTTCGCCGAAAATCTCGCGATCTCGCACAAGGCGCACAACATCAAGGTCTCGATCCTGTGCCCGCAGGG
CGTCGACACCAACATGCTGCGCGGGCTGCCGAAGGGCCCGCAATCCGCCGACGGCGATCTCAGCCCCGAGCAGGTCGCGC
AGGACGTGATCGAGGGGCTCGCCGAAGAGAGCTTCCTGATCCTACCGCACAAGCAGGTGATCGACTACATGCGCAAGAAG
ACCGAGAACTACGACCGCTGGATCGGCGGCATGGCCAAGATCCAGGCGAAATTGCGCGAGACGTTCGGCGCGAAGGGGTA
G

Upstream 100 bases:

>100_bases
CGCTGTTCCCGTTGCGCATATCCCGGCTGCAAAACCGGGTGCCACTTCGGCCGGATCCGCGCTAAGACACCCCCAACAAC
AACCATTCGGGGGAAAAATC

Downstream 100 bases:

>100_bases
TTGTTCGACGTTGCCGCGCGCTCTGCCTAACCTCTCCCCGCGCGCGGGGAGAGGTCGACCGGCGAAGCGTAGCGCAGCCG
GTCGGGTGAGGGGGCGTCTC

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MQVAGKVVVVTGGGNGIGQAMCEAFAKAGAAKVVVADLDEARAEAVAAAIGGAAFKCDVAKESDIKHVIDETERRFGPIA
LFCSNAGIGGGFDPLSENAGGASDEPFMNSWMIHVMAHVYAARHLVPLYRARGGGYFLNTISAAGLLSQVGSPAYSATKH
GAVGFAENLAISHKAHNIKVSILCPQGVDTNMLRGLPKGPQSADGDLSPEQVAQDVIEGLAEESFLILPHKQVIDYMRKK
TENYDRWIGGMAKIQAKLRETFGAKG

Sequences:

>Translated_266_residues
MQVAGKVVVVTGGGNGIGQAMCEAFAKAGAAKVVVADLDEARAEAVAAAIGGAAFKCDVAKESDIKHVIDETERRFGPIA
LFCSNAGIGGGFDPLSENAGGASDEPFMNSWMIHVMAHVYAARHLVPLYRARGGGYFLNTISAAGLLSQVGSPAYSATKH
GAVGFAENLAISHKAHNIKVSILCPQGVDTNMLRGLPKGPQSADGDLSPEQVAQDVIEGLAEESFLILPHKQVIDYMRKK
TENYDRWIGGMAKIQAKLRETFGAKG
>Mature_266_residues
MQVAGKVVVVTGGGNGIGQAMCEAFAKAGAAKVVVADLDEARAEAVAAAIGGAAFKCDVAKESDIKHVIDETERRFGPIA
LFCSNAGIGGGFDPLSENAGGASDEPFMNSWMIHVMAHVYAARHLVPLYRARGGGYFLNTISAAGLLSQVGSPAYSATKH
GAVGFAENLAISHKAHNIKVSILCPQGVDTNMLRGLPKGPQSADGDLSPEQVAQDVIEGLAEESFLILPHKQVIDYMRKK
TENYDRWIGGMAKIQAKLRETFGAKG

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40807363, Length=238, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310128830, Length=255, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310115468, Length=255, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI142976729, Length=277, Percent_Identity=27.4368231046931, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI25282469, Length=273, Percent_Identity=23.8095238095238, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI210032110, Length=248, Percent_Identity=25.8064516129032, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40254992, Length=195, Percent_Identity=31.2820512820513, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI31542939, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4503817, Length=236, Percent_Identity=26.6949152542373, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI59889578, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI32483357, Length=198, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=28.2485875706215, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787905, Length=204, Percent_Identity=28.4313725490196, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787335, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789208, Length=202, Percent_Identity=28.2178217821782, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082100, Length=214, Percent_Identity=26.6355140186916, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=237, Percent_Identity=28.6919831223629, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193203117, Length=237, Percent_Identity=27.8481012658228, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193203115, Length=235, Percent_Identity=28.0851063829787, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17508895, Length=234, Percent_Identity=26.0683760683761, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=212, Percent_Identity=30.6603773584906, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=215, Percent_Identity=31.1627906976744, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=217, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=200, Percent_Identity=27, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=199, Percent_Identity=30.6532663316583, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581726, Length=242, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640492, Length=208, Percent_Identity=28.3653846153846, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355631, Length=173, Percent_Identity=26.5895953757225, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737361, Length=205, Percent_Identity=28.780487804878, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358495, Length=276, Percent_Identity=23.1884057971014, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355319, Length=213, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27938; Mature: 27938

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVAGKVVVVTGGGNGIGQAMCEAFAKAGAAKVVVADLDEARAEAVAAAIGGAAFKCDVA
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEECCC
KESDIKHVIDETERRFGPIALFCSNAGIGGGFDPLSENAGGASDEPFMNSWMIHVMAHVY
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AARHLVPLYRARGGGYFLNTISAAGLLSQVGSPAYSATKHGAVGFAENLAISHKAHNIKV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHEEEECCCEEEE
SILCPQGVDTNMLRGLPKGPQSADGDLSPEQVAQDVIEGLAEESFLILPHKQVIDYMRKK
EEECCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHH
TENYDRWIGGMAKIQAKLRETFGAKG
CCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQVAGKVVVVTGGGNGIGQAMCEAFAKAGAAKVVVADLDEARAEAVAAAIGGAAFKCDVA
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEECCC
KESDIKHVIDETERRFGPIALFCSNAGIGGGFDPLSENAGGASDEPFMNSWMIHVMAHVY
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AARHLVPLYRARGGGYFLNTISAAGLLSQVGSPAYSATKHGAVGFAENLAISHKAHNIKV
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHEEEECCCEEEE
SILCPQGVDTNMLRGLPKGPQSADGDLSPEQVAQDVIEGLAEESFLILPHKQVIDYMRKK
EEECCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHH
TENYDRWIGGMAKIQAKLRETFGAKG
CCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]