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The map label for this gene is fixJ [H]

Identifier: 86750958

GI number: 86750958

Start: 4402751

End: 4403380

Strand: Reverse

Name: fixJ [H]

Synonym: RPB_3850

Alternate gene names: 86750958

Gene position: 4403380-4402751 (Counterclockwise)

Preceding gene: 86750961

Following gene: 86750956

Centisome position: 82.59

GC content: 65.4

Gene sequence:

>630_bases
ATGAATGAGAAGCCAGCAGCCCGGGAGGTGTTCGTCGTCGACGACGATCCGGCCGTGCGCGAGACGCTTTCGATTGTGCT
GTCCACGGCGGGCTACGAGGTGATCTGCTTCGCCGACGGCGAATCTCTGCTCGCGGTGGCGCGCAGCCGCAGTCCCGCCT
GTATCCTGCTCGATGTCCATATTCCGGGGCGGTCAGGGCTCGACATTCTGGCCGAGCTGCACGCCGAGGATTATCCGGCG
CCGATCTTCATGATTTCTGGCAAGGGCGATATCGCGATGGCGGTCAATGCCATCAAGAACGGCGCGCTGGATTTCATCGA
GAAGCCGTTCCGCGGCAAGGAGATCGTCGCCCGGGTCGAGGAGGCGATCGACGCCTTCCAGCGCCGGCGCGGGCCCGGCA
CGGAAGTGAAGGCGCCGTCCTATGTCTTCCCGGGCAAGGAGCCGCTGACGCTGCGGGAGCGGGAGGTGCTGGAGTTGTTC
GCGTCGGGCAACACCAACAAGGAGGCGGGCCGCCAGCTCGGCATCAGCCCGCGCACCATCGAGTATCACCGCGCCAACAT
CATGAAGAAGCTCGGCGCGCGCAACGCCACCGACCTGGTTCGGATCGTGATGACCGCGCCCAAGGCCTGA

Upstream 100 bases:

>100_bases
TTGTTGTAACGTTTTAGTGCCGTCTGAGAAGTATCTTGGAGCCTCGTATAGATACGGGGCGTGGGCTTAGAGTTCGTTCA
AGTGTTTTGGAGGTCGATTG

Downstream 100 bases:

>100_bases
CGGCGGCGCGGGCCGCCCCCGTCACAGCGTGCCGGGGAAGGCGCCGCCGTCGAGCAGGATGTTCTGGCCGGTGATGTAGC
CGGATTTTGCGCCGCACAAG

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNEKPAAREVFVVDDDPAVRETLSIVLSTAGYEVICFADGESLLAVARSRSPACILLDVHIPGRSGLDILAELHAEDYPA
PIFMISGKGDIAMAVNAIKNGALDFIEKPFRGKEIVARVEEAIDAFQRRRGPGTEVKAPSYVFPGKEPLTLREREVLELF
ASGNTNKEAGRQLGISPRTIEYHRANIMKKLGARNATDLVRIVMTAPKA

Sequences:

>Translated_209_residues
MNEKPAAREVFVVDDDPAVRETLSIVLSTAGYEVICFADGESLLAVARSRSPACILLDVHIPGRSGLDILAELHAEDYPA
PIFMISGKGDIAMAVNAIKNGALDFIEKPFRGKEIVARVEEAIDAFQRRRGPGTEVKAPSYVFPGKEPLTLREREVLELF
ASGNTNKEAGRQLGISPRTIEYHRANIMKKLGARNATDLVRIVMTAPKA
>Mature_209_residues
MNEKPAAREVFVVDDDPAVRETLSIVLSTAGYEVICFADGESLLAVARSRSPACILLDVHIPGRSGLDILAELHAEDYPA
PIFMISGKGDIAMAVNAIKNGALDFIEKPFRGKEIVARVEEAIDAFQRRRGPGTEVKAPSYVFPGKEPLTLREREVLELF
ASGNTNKEAGRQLGISPRTIEYHRANIMKKLGARNATDLVRIVMTAPKA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=140, Percent_Identity=32.8571428571429, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789809, Length=149, Percent_Identity=31.5436241610738, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=31.4432989690722, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=209, Percent_Identity=29.1866028708134, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=27.9661016949153, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=26.8518518518519, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788521, Length=202, Percent_Identity=28.7128712871287, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=22.7722772277228, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=26.5625, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788394, Length=210, Percent_Identity=25.2380952380952, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788712, Length=194, Percent_Identity=23.1958762886598, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787229, Length=137, Percent_Identity=26.2773722627737, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI87082012, Length=111, Percent_Identity=29.7297297297297, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22799; Mature: 22799

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKPAAREVFVVDDDPAVRETLSIVLSTAGYEVICFADGESLLAVARSRSPACILLDVH
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHCCCCCEEEEEEEE
IPGRSGLDILAELHAEDYPAPIFMISGKGDIAMAVNAIKNGALDFIEKPFRGKEIVARVE
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHCCCHHHHHCCCCCHHHHHHHH
EAIDAFQRRRGPGTEVKAPSYVFPGKEPLTLREREVLELFASGNTNKEAGRQLGISPRTI
HHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEE
EYHRANIMKKLGARNATDLVRIVMTAPKA
HHHHHHHHHHHCCCCHHHHHHHEECCCCC
>Mature Secondary Structure
MNEKPAAREVFVVDDDPAVRETLSIVLSTAGYEVICFADGESLLAVARSRSPACILLDVH
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHCCCCCEEEEEEEE
IPGRSGLDILAELHAEDYPAPIFMISGKGDIAMAVNAIKNGALDFIEKPFRGKEIVARVE
CCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHCCCHHHHHCCCCCHHHHHHHH
EAIDAFQRRRGPGTEVKAPSYVFPGKEPLTLREREVLELFASGNTNKEAGRQLGISPRTI
HHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEE
EYHRANIMKKLGARNATDLVRIVMTAPKA
HHHHHHHHHHHCCCCHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]