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The map label for this gene is prpB [C]

Identifier: 86750832

GI number: 86750832

Start: 4259227

End: 4260048

Strand: Reverse

Name: prpB [C]

Synonym: RPB_3722

Alternate gene names: 86750832

Gene position: 4260048-4259227 (Counterclockwise)

Preceding gene: 86750833

Following gene: 162138519

Centisome position: 79.9

GC content: 70.56

Gene sequence:

>822_bases
ATGCCCACCTCCACTGCCGCCAAGCGCGCCGCCTTCCGCAAGCTGCACGCCTCCGGCTGCTTCGTGCTGCCGAATCCGTG
GGACGTAGGCTCCGCGCGGCTGCTGCAGCATCTCGGCTTTCAGGCGCTGGCCTCGACCAGTTCGGGCTATGCGTGGTCGA
CCGGGCGGGCCGACAATCATGTCACCTGCGACGACGTGCTGGCACATCTGACGACGCTGAGCGCCGCGGTCGATCTGCCG
ATCAACGCCGATTTCGAGGGCGGCTTCGCCGATGCGCCGGAGGGCGTCGCCGCCAATGTCACCCGCGCGGTGGCGACCGG
GATCGCCGGGCTGTCGATCGAGAATTCGACCGGCGACGCGGCGCGGCCGCTGTATGACGACGCGCTCGCCGTCGAGCGCA
TCAGGGCGGCGCGGGCGGCGATCGATGCCACCGGCGAGGATGTGCTGCTGGTGGCGCGCTGCGAGGGGTTTCTGTGCGGC
GAGCGCGATCTGGCGAAGACCACGGCGCGGCTGGTCGCCTTCGCCGACGCCGGCGCCGATTGCCTGTATGCGCCGGGCGT
GTCGACCGAAGCCGAGATCACCACGCTGGTGCAGGCGATCGCGCCGAAGCCGCTCAACGTGCTGATCGTGCAGCCGACGA
TGAGCGTCGCCGCACTCGCCGACCTCGGCGTCCGCCGCATCAGCGTCGGCGGCGCATTGGCGCGGTCGGCCTGGGCCGGG
TTTCTCAACGCGGCGCGCGAGATCGCCGAGGAGGGCACGTTCACGGCGTTTGCGGGCGCGACGAAGGGTGCGGAGTTGAA
CGGGTTGTTTGGGGGGAAGTAG

Upstream 100 bases:

>100_bases
GGATGGGCTGTCGCAGGGTGGGACACTTTCGGCCGCGCGGAGCCCGATCGAAACAGCGCGCGCCGTGCTACCCTGCCGCA
AAAATCAGGGAGTCACGCCG

Downstream 100 bases:

>100_bases
CGGCTGTCATTGGCAGACGCGTCGAAGTCTTGGCAGTCGACGCCAAGGAGTTCTGTTATCTCTGATCTATTTGCAATGTC
TTGCTATTGCATTGTTGGTG

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPTSTAAKRAAFRKLHASGCFVLPNPWDVGSARLLQHLGFQALASTSSGYAWSTGRADNHVTCDDVLAHLTTLSAAVDLP
INADFEGGFADAPEGVAANVTRAVATGIAGLSIENSTGDAARPLYDDALAVERIRAARAAIDATGEDVLLVARCEGFLCG
ERDLAKTTARLVAFADAGADCLYAPGVSTEAEITTLVQAIAPKPLNVLIVQPTMSVAALADLGVRRISVGGALARSAWAG
FLNAAREIAEEGTFTAFAGATKGAELNGLFGGK

Sequences:

>Translated_273_residues
MPTSTAAKRAAFRKLHASGCFVLPNPWDVGSARLLQHLGFQALASTSSGYAWSTGRADNHVTCDDVLAHLTTLSAAVDLP
INADFEGGFADAPEGVAANVTRAVATGIAGLSIENSTGDAARPLYDDALAVERIRAARAAIDATGEDVLLVARCEGFLCG
ERDLAKTTARLVAFADAGADCLYAPGVSTEAEITTLVQAIAPKPLNVLIVQPTMSVAALADLGVRRISVGGALARSAWAG
FLNAAREIAEEGTFTAFAGATKGAELNGLFGGK
>Mature_272_residues
PTSTAAKRAAFRKLHASGCFVLPNPWDVGSARLLQHLGFQALASTSSGYAWSTGRADNHVTCDDVLAHLTTLSAAVDLPI
NADFEGGFADAPEGVAANVTRAVATGIAGLSIENSTGDAARPLYDDALAVERIRAARAAIDATGEDVLLVARCEGFLCGE
RDLAKTTARLVAFADAGADCLYAPGVSTEAEITTLVQAIAPKPLNVLIVQPTMSVAALADLGVRRISVGGALARSAWAGF
LNAAREIAEEGTFTAFAGATKGAELNGLFGGK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28014; Mature: 27883

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSTAAKRAAFRKLHASGCFVLPNPWDVGSARLLQHLGFQALASTSSGYAWSTGRADNH
CCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCHHHHHCCCCCCEEECCCCCCC
VTCDDVLAHLTTLSAAVDLPINADFEGGFADAPEGVAANVTRAVATGIAGLSIENSTGDA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
ARPLYDDALAVERIRAARAAIDATGEDVLLVARCEGFLCGERDLAKTTARLVAFADAGAD
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCC
CLYAPGVSTEAEITTLVQAIAPKPLNVLIVQPTMSVAALADLGVRRISVGGALARSAWAG
EEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCHHEEECCHHHHHHHHHH
FLNAAREIAEEGTFTAFAGATKGAELNGLFGGK
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTSTAAKRAAFRKLHASGCFVLPNPWDVGSARLLQHLGFQALASTSSGYAWSTGRADNH
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCHHHHHCCCCCCEEECCCCCCC
VTCDDVLAHLTTLSAAVDLPINADFEGGFADAPEGVAANVTRAVATGIAGLSIENSTGDA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCC
ARPLYDDALAVERIRAARAAIDATGEDVLLVARCEGFLCGERDLAKTTARLVAFADAGAD
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEECCCCC
CLYAPGVSTEAEITTLVQAIAPKPLNVLIVQPTMSVAALADLGVRRISVGGALARSAWAG
EEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCHHEEECCHHHHHHHHHH
FLNAAREIAEEGTFTAFAGATKGAELNGLFGGK
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]