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The map label for this gene is purC1 [H]

Identifier: 162138519

GI number: 162138519

Start: 4256483

End: 4257250

Strand: Reverse

Name: purC1 [H]

Synonym: RPB_3719

Alternate gene names: 162138519

Gene position: 4257250-4256483 (Counterclockwise)

Preceding gene: 86750832

Following gene: 86750828

Centisome position: 79.85

GC content: 61.72

Gene sequence:

>768_bases
ATGAGCCGGCGACGTCGCATTTATGAAGGCAAGGCCAAGGTGCTGTATGAAGGCCCCGAACCGGGAACCCTGATTCAGCA
CTTCAAGGACGACGCTACCGCGTTCAACGCCAAGAAGCATCAGGTGATCGAGGGCAAGGGTGTCCTCAACAACCGGATCT
CGGAGTACCTGTTTCAGCACCTCAACGACATCGGGGTGCCGACGCATTTCATCCGCCGGCTGAACATGCGCGAGCAGCTG
ATCCGCGAAGTCGAGATCGTGCCGCTCGAAGTGGTGGTGCGCAACGTCGCCGCCGGTTCGCTGTCGCAGCGGCTCGGGAT
CGAGGAGGGCACCCAATTGCCGCGCTCGATCATCGAGTTCTACTACAAAAACGACCAGCTCAACGACCCGATGGTCTCCG
AGGAGCACATCACCGCGTTCGGCTGGGCGACCCCGCAGGAGATCGACGACATCATGGCGCTGGCGATCCGCGTCAATGAT
TTCCTCACCGGGCTGTTCCTCGGCATCGGCATCCGCCTGGTCGACTTCAAGATGGAATGCGGCCGATTGTTCGAAAACGA
CATGATGCGGATCATCGTCGCCGACGAGATCTCGCCGGATTCGTGCCGGCTGTGGGACATCAAGTCGAACGAGAAGCTCG
ACAAGGACCGCTTCCGCCGCGACCTCGGCGGCCTGCTGGAGGCCTATACCGAAGTCGCCAAGCGCCTCGGCATCCTGATG
GAAAACGAGCGCCCGGCCGGCTCCGGCCCGGTGCTGGTGAAGGGCTGA

Upstream 100 bases:

>100_bases
TCTGTTTCCTGTTCCGGCCTTCACGGCAGCTCCGCCGTGGGTCCCCATCGTCAGACGGAGCCACCCATCCATGGATTTCA
ACAAAACACGGTACATCCCC

Downstream 100 bases:

>100_bases
CAGGCCCCACCGGAGCCGTCGTCCTGAGCCGCATCCGCGGCTCGTCATCCTGAGGTGCGAGCGCAAGCGAGCCTCGAAGG
ATGGCCGCCGCGGGCACGGT

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase A [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQL
IREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVND
FLTGLFLGIGIRLVDFKMECGRLFENDMMRIIVADEISPDSCRLWDIKSNEKLDKDRFRRDLGGLLEAYTEVAKRLGILM
ENERPAGSGPVLVKG

Sequences:

>Translated_255_residues
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQL
IREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVND
FLTGLFLGIGIRLVDFKMECGRLFENDMMRIIVADEISPDSCRLWDIKSNEKLDKDRFRRDLGGLLEAYTEVAKRLGILM
ENERPAGSGPVLVKG
>Mature_254_residues
SRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQLI
REVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVNDF
LTGLFLGIGIRLVDFKMECGRLFENDMMRIIVADEISPDSCRLWDIKSNEKLDKDRFRRDLGGLLEAYTEVAKRLGILME
NERPAGSGPVLVKG

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=256, Percent_Identity=32.421875, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI5453539, Length=256, Percent_Identity=32.421875, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI119220559, Length=263, Percent_Identity=31.1787072243346, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788820, Length=241, Percent_Identity=41.0788381742739, Blast_Score=204, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17531275, Length=243, Percent_Identity=30.0411522633745, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI18860083, Length=237, Percent_Identity=29.535864978903, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583917, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 29142; Mature: 29011

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01057 SAICAR_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQH
CCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHH
LNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVNDFLTGLFLGIGIRLVDFKMEC
HHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHH
GRLFENDMMRIIVADEISPDSCRLWDIKSNEKLDKDRFRRDLGGLLEAYTEVAKRLGILM
HHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
ENERPAGSGPVLVKG
CCCCCCCCCCEEECC
>Mature Secondary Structure 
SRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQH
CCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHH
LNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVNDFLTGLFLGIGIRLVDFKMEC
HHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHH
GRLFENDMMRIIVADEISPDSCRLWDIKSNEKLDKDRFRRDLGGLLEAYTEVAKRLGILM
HHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEE
ENERPAGSGPVLVKG
CCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]