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The map label for this gene is glxR [C]

Identifier: 86750505

GI number: 86750505

Start: 3879868

End: 3880737

Strand: Direct

Name: glxR [C]

Synonym: RPB_3394

Alternate gene names: 86750505

Gene position: 3879868-3880737 (Clockwise)

Preceding gene: 86750502

Following gene: 86750506

Centisome position: 72.77

GC content: 68.16

Gene sequence:

>870_bases
ATGGCCAAAGTCGCTTTCCTCGGTCTCGGCGTGATGGGTTTTCCGATGGCCGGACATCTCGTCAAGAAGGGCGGCCACGA
GGTCACCGTCTACAACCGGACCGCGGCCAAGGCCAAGGCCTGGGCCGATCAATTCGGCGGCCGCATTGCGGCGACGCCGG
CGGAAGCGGCGCAGGATCAGGACTTCGTGATGGCCTGCGTCGGCAACGACGACGATCTGCGCGCCGTCACCACCGGCGCC
GACGGCGCCTTCGCGGCGATGGCATCCGGCGCGGTGTTCGTCGATCACACCACCGCCTCCGCCGAGGTGGCGCGCGAGCT
CGACGCCGCCGCGACCAAGGCCGGCTTCGCCTTCGTCGATGCGCCGGTCTCGGGCGGTCAGGCCGGCGCGCAGAACGGCG
TGCTGACGGTGATGTGCGGCGGCAGCGACGCGGCCTACGCCAAGGCCGAGCCGGTGATCGCGGCCTATGCCCGGATGTGC
AAGCTGCTCGGCCCGGCCGGCTCCGGCCAGCTCACCAAGATGGTCAACCAGATCTGCATCGCCGGACTGGTGCAGGGACT
GTCGGAAGGCATTCACTTCGCCAAGCGGGCCGGGCTCGACGTCAATGCGGTGATCGACACCATCTCCAAGGGCGCCGCAC
AGTCCTGGCAGATGGAGAACCGCCACAAGACCATGATCGACGGCAAGTACGATTTCGGCTTCGCGGTCGAATGGATGCGC
AAGGATCTGTCGATCTGCCTCGCCGAGTCGCGCCGCAACGGCGCCAGCCTGCCGGTCACCGGCCTGGTCGACGCCTTCTA
TGCCGAAGTCGAGAAGCTCGGCGGCCGCCGCTGGGATACATCGAGCCTGCTGGCGCGGCTCGAACGCTGA

Upstream 100 bases:

>100_bases
TGCTGGCGCACCTGCCGCGCGCCTGCATCGGGCCGCGCGCGCTCCCTCCCCGTCATTCGGCCGGCCTGCGTCCGGCCACG
CAATCCTGAAGGGTACAGAC

Downstream 100 bases:

>100_bases
GACGCAGAGACTAGAAAATCGCCGGCGTTTGCGGTTTTTCACGCGGCGATAACCGCAATCGTTAGCCAGCCGTTAACCGG
AATATCATTCCCTCGGAGGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAKVAFLGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKAWADQFGGRIAATPAEAAQDQDFVMACVGNDDDLRAVTTGA
DGAFAAMASGAVFVDHTTASAEVARELDAAATKAGFAFVDAPVSGGQAGAQNGVLTVMCGGSDAAYAKAEPVIAAYARMC
KLLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKRAGLDVNAVIDTISKGAAQSWQMENRHKTMIDGKYDFGFAVEWMR
KDLSICLAESRRNGASLPVTGLVDAFYAEVEKLGGRRWDTSSLLARLER

Sequences:

>Translated_289_residues
MAKVAFLGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKAWADQFGGRIAATPAEAAQDQDFVMACVGNDDDLRAVTTGA
DGAFAAMASGAVFVDHTTASAEVARELDAAATKAGFAFVDAPVSGGQAGAQNGVLTVMCGGSDAAYAKAEPVIAAYARMC
KLLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKRAGLDVNAVIDTISKGAAQSWQMENRHKTMIDGKYDFGFAVEWMR
KDLSICLAESRRNGASLPVTGLVDAFYAEVEKLGGRRWDTSSLLARLER
>Mature_288_residues
AKVAFLGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKAWADQFGGRIAATPAEAAQDQDFVMACVGNDDDLRAVTTGAD
GAFAAMASGAVFVDHTTASAEVARELDAAATKAGFAFVDAPVSGGQAGAQNGVLTVMCGGSDAAYAKAEPVIAAYARMCK
LLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKRAGLDVNAVIDTISKGAAQSWQMENRHKTMIDGKYDFGFAVEWMRK
DLSICLAESRRNGASLPVTGLVDAFYAEVEKLGGRRWDTSSLLARLER

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=246, Percent_Identity=28.0487804878049, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=26.962457337884, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=29.3706293706294, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=30.7958477508651, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=26.8707482993197, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=27.7966101694915, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=28.3636363636364, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=27.5167785234899, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=27.5167785234899, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=22.6950354609929, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30170; Mature: 30038

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFLGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKAWADQFGGRIAATPAEAAQDQ
CCEEEEECCCHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCEEEECCHHHCCCC
DFVMACVGNDDDLRAVTTGADGAFAAMASGAVFVDHTTASAEVARELDAAATKAGFAFVD
CEEEEEECCCCCCEEEECCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
APVSGGQAGAQNGVLTVMCGGSDAAYAKAEPVIAAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVQGLSEGIHFAKRAGLDVNAVIDTISKGAAQSWQMENRHKTMIDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEECCCCCCHHHHHHHH
KDLSICLAESRRNGASLPVTGLVDAFYAEVEKLGGRRWDTSSLLARLER
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKVAFLGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKAWADQFGGRIAATPAEAAQDQ
CEEEEECCCHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCEEEECCHHHCCCC
DFVMACVGNDDDLRAVTTGADGAFAAMASGAVFVDHTTASAEVARELDAAATKAGFAFVD
CEEEEEECCCCCCEEEECCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
APVSGGQAGAQNGVLTVMCGGSDAAYAKAEPVIAAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVQGLSEGIHFAKRAGLDVNAVIDTISKGAAQSWQMENRHKTMIDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEECCCCCCHHHHHHHH
KDLSICLAESRRNGASLPVTGLVDAFYAEVEKLGGRRWDTSSLLARLER
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA