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The map label for this gene is moaA [H]

Identifier: 86750500

GI number: 86750500

Start: 3873127

End: 3874164

Strand: Direct

Name: moaA [H]

Synonym: RPB_3389

Alternate gene names: 86750500

Gene position: 3873127-3874164 (Clockwise)

Preceding gene: 86750499

Following gene: 86750501

Centisome position: 72.64

GC content: 67.92

Gene sequence:

>1038_bases
ATGATCGATGCTGACGTGACGCCCCCGCCGACCGCCGGCGCTTCGACGATGGTCGATCCGTTCGGCCGGACCATCGACTA
TCTGCGGGTCTCGATCACCGACCGCTGCGATTTTCGCTGCGCGTACTGCATGTCCGAGGACATGACCTTCCTGCCCCGCG
CCGATCTGCTGACGCTGGAGGAACTCGACCGGCTGTGTTCGGCCTTCATCGTCCGGGGCGTGCGCAAGCTCAGGCTCACC
GGCGGCGAGCCGCTGGTCCGGCGCAACATGATGTCGCTGGTGCGGTCGCTCTCCCGCCATCTCGACACCGGCGCGCTGCG
CGAACTCACCCTCACCACCAACGGATCACAGCTCGCCCGCTTCGCCGCCGAACTACGCGACTGCGGGGTTCGGCGCATCA
ACGTCTCGCTCGACACGCTTGATCCGGCGAAGTTCCGCGCGATCACCCGCTGGGGCGAATTCGACCGGGTGATCGCCGGC
ATCGAGGCCGCGCGCGCGGCCGGCCTCGCCGTCAAGATCAACGCGGTGGTGCTGAAGGGCGTCAACGAGGACGAGATCCC
CGCCTTGATGCAATGGGCGCACGGGCTCGGCATGGGGCTGACGCTGATCGAGGTGATGCCGCTGGGCGAGATCGGCGAAG
GCCGGATCGACCAGTACGTGCCGCTGTCGCTGGTTCGCGCCCGGCTGTCGAACAACTACACGCTGACGGATCTGCCCGAC
AGCACCGGCGGCCCGGCCCGCTACGTCCGGGTCGAGGAGACCGGCGGCAAGCTCGGATTCATCACCCCCCTCACCCACAA
TTTCTGCGAATCCTGCAACCGGGTTCGGATCACCTGCACCGGCACGCTGCACACCTGCCTCGGCCAGGAAGACGCCGCCG
ATCTGCGCCGGCCGCTGCGCGCCTCGCCCGACGATGCGCTGCTCAACGCCGCGATCGATCGCGCCATCGGCCACAAGCCC
AAGGGCCACGACTTCATCATCGACCGCAAACACGACCGGCCGAGCGTCAGCCGGCATATGAGCGTGACCGGGGGCTGA

Upstream 100 bases:

>100_bases
CTGCAACGCAGCATGCCGCGCGATTGTGTGCGGCGACAATCCTGCATAGATAGTGGTGAGCTCCATGCCGCCACGGCGGC
GCTTGTGACAGGGCCGCCGG

Downstream 100 bases:

>100_bases
GCCCGCCGAAACCGCAAGTCACCTGGAATCCACAAGTCACCTGGAATCCACAAGTCACCTGGAATCCGCAAATCGTCCTG
CCGCGTATTTAACCGTTTGA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIDADVTPPPTAGASTMVDPFGRTIDYLRVSITDRCDFRCAYCMSEDMTFLPRADLLTLEELDRLCSAFIVRGVRKLRLT
GGEPLVRRNMMSLVRSLSRHLDTGALRELTLTTNGSQLARFAAELRDCGVRRINVSLDTLDPAKFRAITRWGEFDRVIAG
IEAARAAGLAVKINAVVLKGVNEDEIPALMQWAHGLGMGLTLIEVMPLGEIGEGRIDQYVPLSLVRARLSNNYTLTDLPD
STGGPARYVRVEETGGKLGFITPLTHNFCESCNRVRITCTGTLHTCLGQEDAADLRRPLRASPDDALLNAAIDRAIGHKP
KGHDFIIDRKHDRPSVSRHMSVTGG

Sequences:

>Translated_345_residues
MIDADVTPPPTAGASTMVDPFGRTIDYLRVSITDRCDFRCAYCMSEDMTFLPRADLLTLEELDRLCSAFIVRGVRKLRLT
GGEPLVRRNMMSLVRSLSRHLDTGALRELTLTTNGSQLARFAAELRDCGVRRINVSLDTLDPAKFRAITRWGEFDRVIAG
IEAARAAGLAVKINAVVLKGVNEDEIPALMQWAHGLGMGLTLIEVMPLGEIGEGRIDQYVPLSLVRARLSNNYTLTDLPD
STGGPARYVRVEETGGKLGFITPLTHNFCESCNRVRITCTGTLHTCLGQEDAADLRRPLRASPDDALLNAAIDRAIGHKP
KGHDFIIDRKHDRPSVSRHMSVTGG
>Mature_345_residues
MIDADVTPPPTAGASTMVDPFGRTIDYLRVSITDRCDFRCAYCMSEDMTFLPRADLLTLEELDRLCSAFIVRGVRKLRLT
GGEPLVRRNMMSLVRSLSRHLDTGALRELTLTTNGSQLARFAAELRDCGVRRINVSLDTLDPAKFRAITRWGEFDRVIAG
IEAARAAGLAVKINAVVLKGVNEDEIPALMQWAHGLGMGLTLIEVMPLGEIGEGRIDQYVPLSLVRARLSNNYTLTDLPD
STGGPARYVRVEETGGKLGFITPLTHNFCESCNRVRITCTGTLHTCLGQEDAADLRRPLRASPDDALLNAAIDRAIGHKP
KGHDFIIDRKHDRPSVSRHMSVTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=330, Percent_Identity=37.2727272727273, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI22027536, Length=330, Percent_Identity=37.2727272727273, Blast_Score=208, Evalue=7e-54,
Organism=Escherichia coli, GI1786999, Length=335, Percent_Identity=34.6268656716418, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566331, Length=337, Percent_Identity=35.0148367952522, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=316, Percent_Identity=36.0759493670886, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17561210, Length=317, Percent_Identity=36.9085173501577, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI28574490, Length=322, Percent_Identity=36.6459627329193, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28574488, Length=329, Percent_Identity=35.8662613981763, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37909; Mature: 37909

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDADVTPPPTAGASTMVDPFGRTIDYLRVSITDRCDFRCAYCMSEDMTFLPRADLLTLE
CCCCCCCCCCCCCCCCHHHHHHCCEEHEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHH
ELDRLCSAFIVRGVRKLRLTGGEPLVRRNMMSLVRSLSRHLDTGALRELTLTTNGSQLAR
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
FAAELRDCGVRRINVSLDTLDPAKFRAITRWGEFDRVIAGIEAARAAGLAVKINAVVLKG
HHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEEEEC
VNEDEIPALMQWAHGLGMGLTLIEVMPLGEIGEGRIDQYVPLSLVRARLSNNYTLTDLPD
CCCCHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCC
STGGPARYVRVEETGGKLGFITPLTHNFCESCNRVRITCTGTLHTCLGQEDAADLRRPLR
CCCCCEEEEEEECCCCCEEEECHHHHHHHHCCCCEEEEEECHHHHHCCCCHHHHHHHCCC
ASPDDALLNAAIDRAIGHKPKGHDFIIDRKHDRPSVSRHMSVTGG
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCEECCCC
>Mature Secondary Structure
MIDADVTPPPTAGASTMVDPFGRTIDYLRVSITDRCDFRCAYCMSEDMTFLPRADLLTLE
CCCCCCCCCCCCCCCCHHHHHHCCEEHEEEEECCCCCCEEEEECCCCCCCCCCCCCCCHH
ELDRLCSAFIVRGVRKLRLTGGEPLVRRNMMSLVRSLSRHLDTGALRELTLTTNGSQLAR
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
FAAELRDCGVRRINVSLDTLDPAKFRAITRWGEFDRVIAGIEAARAAGLAVKINAVVLKG
HHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEEEEC
VNEDEIPALMQWAHGLGMGLTLIEVMPLGEIGEGRIDQYVPLSLVRARLSNNYTLTDLPD
CCCCHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCC
STGGPARYVRVEETGGKLGFITPLTHNFCESCNRVRITCTGTLHTCLGQEDAADLRRPLR
CCCCCEEEEEEECCCCCEEEECHHHHHHHHCCCCEEEEEECHHHHHCCCCHHHHHHHCCC
ASPDDALLNAAIDRAIGHKPKGHDFIIDRKHDRPSVSRHMSVTGG
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA