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The map label for this gene is cobB [H]

Identifier: 86750296

GI number: 86750296

Start: 3641728

End: 3643089

Strand: Direct

Name: cobB [H]

Synonym: RPB_3185

Alternate gene names: 86750296

Gene position: 3641728-3643089 (Clockwise)

Preceding gene: 86750295

Following gene: 86750297

Centisome position: 68.3

GC content: 70.7

Gene sequence:

>1362_bases
GTGCCCGCGACTGATCGCACAACCAGCGCTGCGAGGCTCGGCACACCGCCGGGGCTGATCATCGCCGCGCCGTCGACCAA
CAGCGGCAAGACCACGGTGACGCTGGCGTTGCTCGCCGCGCTGCGTCGCCGCGGCCGCATCGTGCAGCCTTACAAATGCG
GTCCCGACTACATCGACCCGGCGTTTCACGCAGCCGCAGCGGGGCGACCCAGCTACAATCTCGACTCCTTCGCCTTTTCG
CCCGGCCGGATCGCCGCGCTGCTCGAGGCGGCAGCCGGCGCGGACCTCTGCATCGCCGAAGGCGTGATGGGGCTGTTCGA
CGGCGGCAGCACGACAGGCGCGTGGGGCAATGGCGCCACCGCCGATATCGCCGCGGCCACCGGCTGGCCGGTGGCGCTGG
TCATCGACGTCGCCGGCCAGTCGCAATCCGCCGCTGCGCTGGCGCTGGGCTTTGCGAACTACCGGAGCGACGTGAGGCTG
GCCGGCGTGATCCTCAACAACGTCGCCAGCGCGCGGCACGAGGCGCTGGTCCGCGCCGGCTTCACGACGCAGAGCATTCC
TGTGCTGGGCACGCTCGCACGACATGCCAGCAGCGCTATCAAGAACCGCCATCTCGGGCTGGTGCAGGCGCGCGAACACG
CGTCGCTGGACGAGCGGCTCGATGTGCTCGCCACCCTGGCCGAACAGGGCATCGACCTCGACGCGCTCGAACGCGCCGCC
GTCCCGTCGCACTTCGATACCCCGGCGACGCCCCGCATCGCACCGCCCGGCCAGCGCATAGCGCTCGCCAGCGACGACGC
CTTCTCCTTCATCTATCCGCATCTGCTCGCCGGATGGCGCGCCGCCGGCGCGGAGATCGTGCCGTTCTCGCCGCTCGCCG
ACGAAGGACCCGATGATACGTGTGATGCGGCCTGGCTGCCGGGCGGCTATCCCGAACTCCATGCCGGCCAACTCGCGGCG
GCGACGCGCTTCAAATCCGCGCTGACGCGCTTTGCGCAGACCCGCCCGGTGCACGGCGAATGCGGAGGCTACATGACGCT
CGGCGCCGGCCTGATCGATGCCGACGGGCAGCGCCACGCGATGGTCGGCTTACTTGGTGTCGAAACCGATTTCGCCCAGC
GCAAGCTGCATCTCGGTTATCGCGTCGCGACGCTGCAGACGCCGGCCGCCGGCCACCCCGCCGGCTGCGTCCTGCGCGGT
CACGAATATCACTATGCCCGCGTGCTCGGCGTGGCCGACGACCCGCTGGCACGGATCTGCGATGCCGCCGGTGCGCCGAC
CAGCGAGACCGGCAGCCGGCGCGGCCACGTGAGCGGGACGTTCTTCCACCTGATCGACGTCGCGCAGGAGCCCGTCGCCT
GA

Upstream 100 bases:

>100_bases
ATCCTGATCGGGCCGTCGCTCGCAGCGAGCGATTTCCGCAATAGCGCGCTCTACAGCACGGACTACGACCGGCGCTACCG
TCGCTCCGCGGAGGACGATC

Downstream 100 bases:

>100_bases
TGCCGACCGCTTCGACCTCCTCTTTCATGCCCGGCAGCGTCGCCCTCGTCGGCGCCGGCCCCGGCGATGCCGACCTGCTG
ACGCTGCGCGCCATCAAGCG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPATDRTTSAARLGTPPGLIIAAPSTNSGKTTVTLALLAALRRRGRIVQPYKCGPDYIDPAFHAAAAGRPSYNLDSFAFS
PGRIAALLEAAAGADLCIAEGVMGLFDGGSTTGAWGNGATADIAAATGWPVALVIDVAGQSQSAAALALGFANYRSDVRL
AGVILNNVASARHEALVRAGFTTQSIPVLGTLARHASSAIKNRHLGLVQAREHASLDERLDVLATLAEQGIDLDALERAA
VPSHFDTPATPRIAPPGQRIALASDDAFSFIYPHLLAGWRAAGAEIVPFSPLADEGPDDTCDAAWLPGGYPELHAGQLAA
ATRFKSALTRFAQTRPVHGECGGYMTLGAGLIDADGQRHAMVGLLGVETDFAQRKLHLGYRVATLQTPAAGHPAGCVLRG
HEYHYARVLGVADDPLARICDAAGAPTSETGSRRGHVSGTFFHLIDVAQEPVA

Sequences:

>Translated_453_residues
MPATDRTTSAARLGTPPGLIIAAPSTNSGKTTVTLALLAALRRRGRIVQPYKCGPDYIDPAFHAAAAGRPSYNLDSFAFS
PGRIAALLEAAAGADLCIAEGVMGLFDGGSTTGAWGNGATADIAAATGWPVALVIDVAGQSQSAAALALGFANYRSDVRL
AGVILNNVASARHEALVRAGFTTQSIPVLGTLARHASSAIKNRHLGLVQAREHASLDERLDVLATLAEQGIDLDALERAA
VPSHFDTPATPRIAPPGQRIALASDDAFSFIYPHLLAGWRAAGAEIVPFSPLADEGPDDTCDAAWLPGGYPELHAGQLAA
ATRFKSALTRFAQTRPVHGECGGYMTLGAGLIDADGQRHAMVGLLGVETDFAQRKLHLGYRVATLQTPAAGHPAGCVLRG
HEYHYARVLGVADDPLARICDAAGAPTSETGSRRGHVSGTFFHLIDVAQEPVA
>Mature_452_residues
PATDRTTSAARLGTPPGLIIAAPSTNSGKTTVTLALLAALRRRGRIVQPYKCGPDYIDPAFHAAAAGRPSYNLDSFAFSP
GRIAALLEAAAGADLCIAEGVMGLFDGGSTTGAWGNGATADIAAATGWPVALVIDVAGQSQSAAALALGFANYRSDVRLA
GVILNNVASARHEALVRAGFTTQSIPVLGTLARHASSAIKNRHLGLVQAREHASLDERLDVLATLAEQGIDLDALERAAV
PSHFDTPATPRIAPPGQRIALASDDAFSFIYPHLLAGWRAAGAEIVPFSPLADEGPDDTCDAAWLPGGYPELHAGQLAAA
TRFKSALTRFAQTRPVHGECGGYMTLGAGLIDADGQRHAMVGLLGVETDFAQRKLHLGYRVATLQTPAAGHPAGCVLRGH
EYHYARVLGVADDPLARICDAAGAPTSETGSRRGHVSGTFFHLIDVAQEPVA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47000; Mature: 46869

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATDRTTSAARLGTPPGLIIAAPSTNSGKTTVTLALLAALRRRGRIVQPYKCGPDYIDP
CCCCCCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCH
AFHAAAAGRPSYNLDSFAFSPGRIAALLEAAAGADLCIAEGVMGLFDGGSTTGAWGNGAT
HHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHCHHHHCCCCCCCCCCCCCCC
ADIAAATGWPVALVIDVAGQSQSAAALALGFANYRSDVRLAGVILNNVASARHEALVRAG
CEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
FTTQSIPVLGTLARHASSAIKNRHLGLVQAREHASLDERLDVLATLAEQGIDLDALERAA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHC
VPSHFDTPATPRIAPPGQRIALASDDAFSFIYPHLLAGWRAAGAEIVPFSPLADEGPDDT
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
CDAAWLPGGYPELHAGQLAAATRFKSALTRFAQTRPVHGECGGYMTLGAGLIDADGQRHA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCCCEE
MVGLLGVETDFAQRKLHLGYRVATLQTPAAGHPAGCVLRGHEYHYARVLGVADDPLARIC
EEEEEECCCHHHHHHHHCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHH
DAAGAPTSETGSRRGHVSGTFFHLIDVAQEPVA
HHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PATDRTTSAARLGTPPGLIIAAPSTNSGKTTVTLALLAALRRRGRIVQPYKCGPDYIDP
CCCCCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCH
AFHAAAAGRPSYNLDSFAFSPGRIAALLEAAAGADLCIAEGVMGLFDGGSTTGAWGNGAT
HHHHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHCHHHHCCCCCCCCCCCCCCC
ADIAAATGWPVALVIDVAGQSQSAAALALGFANYRSDVRLAGVILNNVASARHEALVRAG
CEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
FTTQSIPVLGTLARHASSAIKNRHLGLVQAREHASLDERLDVLATLAEQGIDLDALERAA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHC
VPSHFDTPATPRIAPPGQRIALASDDAFSFIYPHLLAGWRAAGAEIVPFSPLADEGPDDT
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
CDAAWLPGGYPELHAGQLAAATRFKSALTRFAQTRPVHGECGGYMTLGAGLIDADGQRHA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCCCEE
MVGLLGVETDFAQRKLHLGYRVATLQTPAAGHPAGCVLRGHEYHYARVLGVADDPLARIC
EEEEEECCCHHHHHHHHCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHH
DAAGAPTSETGSRRGHVSGTFFHLIDVAQEPVA
HHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]