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The map label for this gene is tyrS [H]

Identifier: 86750115

GI number: 86750115

Start: 3412469

End: 3413725

Strand: Direct

Name: tyrS [H]

Synonym: RPB_2998

Alternate gene names: 86750115

Gene position: 3412469-3413725 (Clockwise)

Preceding gene: 86750113

Following gene: 86750116

Centisome position: 64.0

GC content: 65.23

Gene sequence:

>1257_bases
ATGACCGCATTTAAGTCTGATTTTATGAACGTGCTCCAGCAGCGGGGCTTCGTTCATCAGATGTCCGACCCGGAATCGCT
GGACGCGCTGGCAGCGCGCGGCGAGGTCGTCGCCTATGTCGGCTACGACTGCACCGCGCCGTCGCTGCATGTCGGTCATC
TCTTGTCGATCATGATGCTGCACTGGCTGCAGGCCACCGGCAACAAGCCGATCGCGCTGATGGGCGGCGGCACCACGCGC
GTCGGCGATCCGTCCGGCCGCGACGAGACCCGCAAGATCCTCACCTACGACCAGATCGACGCCAACAAGGAGTCGATCAA
GGGCACGTTCGCGAAGTTCATCAAGTTCGGCGACGGCGCCAGCGACGCGCTGATGGCCGACAACGCCGAATGGCTGACCA
AGCTGAACTACATCGAGATGCTGCGCGACATCGGCCGCCACTTCTCGATCAACCGCATGCTGACGATGGATTCGGTGCGG
ATGCGGCTCGAGCGCGAGCAGGAGCTGTCGTTCATCGAGTTCAATTACATGATCCTGCAGTCCTACGACTTCGTCGAGCT
GGCGCGGCGCTACAATTGCAACCTTCAGATGGGCGGCTCCGACCAGTGGGGCAACATCGTCACCGGCGTCGACCTCGGCC
GCCGCATGGGGACGCACCAGCTTCACGCGCTGACTTGCCCGCTGCTGACGACATCGTCCGGCGCCAAGATGGGCAAGACC
GCCGCCGGCGCGGTCTGGCTCAACGGCGACATGCTGGCGCCCTACGACTACTGGCAGTACTGGCGCAACACCGAGGACGC
CGACGTCGGCCGCTTCCTCAAACTGTTCACGCTGCTGCCGATCGACGAGGTCGAACGGCTGGCGAAGCTGCAGGGAGCCG
AGATCAACGAGGCGAAGAAGGTGCTCGCCACCGAGGCGACCGCACTGATGCACGGCCGTGAGACGGCGGAGAAGGCGGAG
GCGACGGCGCGGACGACGTTCGAACAAGGCGGCACCGCGCAGGACCTGCCGAGCATCGACGTCACCCGCGCCGAGCTCGA
CGCCGGGATCGGCGTGCTCGCGGCGTTCGCCGAGAAGGCCGGCCTCGTCGCCTCCAACGGCGAGGCGCGGCGGCAGATCA
AGGCCGGCGGCCTGCGGGTCAACGACGTCCTCGTGACCGACGAGAAGATGACGCTGACCCCGGCAGATCTCACCCCTGAG
GGCGTGATCAAGCTGTCGATGGGCAAGAAGAAGCACGTCCTGCTCAGGCTCGCATAG

Upstream 100 bases:

>100_bases
TTATAATGCCACACGGTAACGCGATCGAAAGCCCTTGCAGGCATTCCGCGCTGCCGATCTGCGGCGAGCGTGAAATAACA
GTGATCAGAGATCCAAAAAG

Downstream 100 bases:

>100_bases
CCCCATTCGCCGGCGGCGCTGTTCATTCCGGCGGAAACGCGCTCCATGGAGCCAACGTTTTCGGGAAGGACCGCACCTTG
GACCAGCGCGCATCGCAGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTAFKSDFMNVLQQRGFVHQMSDPESLDALAARGEVVAYVGYDCTAPSLHVGHLLSIMMLHWLQATGNKPIALMGGGTTR
VGDPSGRDETRKILTYDQIDANKESIKGTFAKFIKFGDGASDALMADNAEWLTKLNYIEMLRDIGRHFSINRMLTMDSVR
MRLEREQELSFIEFNYMILQSYDFVELARRYNCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTSSGAKMGKT
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPIDEVERLAKLQGAEINEAKKVLATEATALMHGRETAEKAE
ATARTTFEQGGTAQDLPSIDVTRAELDAGIGVLAAFAEKAGLVASNGEARRQIKAGGLRVNDVLVTDEKMTLTPADLTPE
GVIKLSMGKKKHVLLRLA

Sequences:

>Translated_418_residues
MTAFKSDFMNVLQQRGFVHQMSDPESLDALAARGEVVAYVGYDCTAPSLHVGHLLSIMMLHWLQATGNKPIALMGGGTTR
VGDPSGRDETRKILTYDQIDANKESIKGTFAKFIKFGDGASDALMADNAEWLTKLNYIEMLRDIGRHFSINRMLTMDSVR
MRLEREQELSFIEFNYMILQSYDFVELARRYNCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTSSGAKMGKT
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPIDEVERLAKLQGAEINEAKKVLATEATALMHGRETAEKAE
ATARTTFEQGGTAQDLPSIDVTRAELDAGIGVLAAFAEKAGLVASNGEARRQIKAGGLRVNDVLVTDEKMTLTPADLTPE
GVIKLSMGKKKHVLLRLA
>Mature_417_residues
TAFKSDFMNVLQQRGFVHQMSDPESLDALAARGEVVAYVGYDCTAPSLHVGHLLSIMMLHWLQATGNKPIALMGGGTTRV
GDPSGRDETRKILTYDQIDANKESIKGTFAKFIKFGDGASDALMADNAEWLTKLNYIEMLRDIGRHFSINRMLTMDSVRM
RLEREQELSFIEFNYMILQSYDFVELARRYNCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTSSGAKMGKTA
AGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPIDEVERLAKLQGAEINEAKKVLATEATALMHGRETAEKAEA
TARTTFEQGGTAQDLPSIDVTRAELDAGIGVLAAFAEKAGLVASNGEARRQIKAGGLRVNDVLVTDEKMTLTPADLTPEG
VIKLSMGKKKHVLLRLA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=413, Percent_Identity=34.3825665859564, Blast_Score=228, Evalue=9e-60,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=38.1861575178998, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25145802, Length=313, Percent_Identity=32.9073482428115, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=250, Percent_Identity=29.6, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325160, Length=442, Percent_Identity=31.447963800905, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=34.2528735632184, Blast_Score=245, Evalue=5e-65,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46132; Mature: 46001

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFKSDFMNVLQQRGFVHQMSDPESLDALAARGEVVAYVGYDCTAPSLHVGHLLSIMML
CCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
HWLQATGNKPIALMGGGTTRVGDPSGRDETRKILTYDQIDANKESIKGTFAKFIKFGDGA
HHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
SDALMADNAEWLTKLNYIEMLRDIGRHFSINRMLTMDSVRMRLEREQELSFIEFNYMILQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCEECCCEEEHHHHHHHHHHHHCCCEEEEEEEEEC
SYDFVELARRYNCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTSSGAKMGKT
CCCHHHHHHHHCCCEEECCCCCCCCEEEHHHHHHHCCCCCEEEEECCEEECCCCCCCCCC
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPIDEVERLAKLQGAEINEAKK
CCCEEEECCCEECCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHH
VLATEATALMHGRETAEKAEATARTTFEQGGTAQDLPSIDVTRAELDAGIGVLAAFAEKA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHC
GLVASNGEARRQIKAGGLRVNDVLVTDEKMTLTPADLTPEGVIKLSMGKKKHVLLRLA
CCEECCCHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TAFKSDFMNVLQQRGFVHQMSDPESLDALAARGEVVAYVGYDCTAPSLHVGHLLSIMML
CCHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
HWLQATGNKPIALMGGGTTRVGDPSGRDETRKILTYDQIDANKESIKGTFAKFIKFGDGA
HHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
SDALMADNAEWLTKLNYIEMLRDIGRHFSINRMLTMDSVRMRLEREQELSFIEFNYMILQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCEECCCEEEHHHHHHHHHHHHCCCEEEEEEEEEC
SYDFVELARRYNCNLQMGGSDQWGNIVTGVDLGRRMGTHQLHALTCPLLTTSSGAKMGKT
CCCHHHHHHHHCCCEEECCCCCCCCEEEHHHHHHHCCCCCEEEEECCEEECCCCCCCCCC
AAGAVWLNGDMLAPYDYWQYWRNTEDADVGRFLKLFTLLPIDEVERLAKLQGAEINEAKK
CCCEEEECCCEECCHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHH
VLATEATALMHGRETAEKAEATARTTFEQGGTAQDLPSIDVTRAELDAGIGVLAAFAEKA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHC
GLVASNGEARRQIKAGGLRVNDVLVTDEKMTLTPADLTPEGVIKLSMGKKKHVLLRLA
CCEECCCHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA