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The map label for this gene is mltB [H]

Identifier: 86750075

GI number: 86750075

Start: 3367955

End: 3369181

Strand: Direct

Name: mltB [H]

Synonym: RPB_2958

Alternate gene names: 86750075

Gene position: 3367955-3369181 (Clockwise)

Preceding gene: 86750074

Following gene: 86750076

Centisome position: 63.17

GC content: 69.19

Gene sequence:

>1227_bases
ATGGCCAGCTTCGATTCCCGCTTCACGCCGACCCGCCGCCGCCTGCTGCAAACCGCGCTCGGCGCGGCTGTGATGTGGCC
GGCCATCGCGCGCGCCGCCCCGGCCGGCTTCGACGCGTGGCGCGACGGGTTCCGCAGCCGCGCGCTCGCCAAGGGCATCA
CGCCGCAGACCTGGGACCGCGCGATGGCCCGGCTCGAGCCGGACATGAGCGTGTTCAAGAATTTCCAGAAGCAGCCCGAA
TTCCACGAGCAGCTCTGGCAATACATCAACCGCCGCGTCTCCGACTGGCGCATCACCAACGGCCGCTTGGCGCTGCAGAA
GACCGAGCCGCTGTTCGCCCGGATCGAGCGCGACTTCGGCGTCGAGCGCGGCACGCTGCTGGCGCTGTGGGGCGTCGAGA
GCGCCTATGGCGATCCCTTGGTGCAGCAGAACCACATGCGGCCGGTGTTTCCCTCGCTCGCGGCGCTGGCCTGGAACGAG
CCGCGCCGCAAAGCCTATTGGGAAACCGAACTGATCAACGCGCTCAGAATCGTCGATCGCGGCTGGTCGACGCCCGAGGA
GATGCGCGGCTCCTGGGCCGGCGCGATGGGGCATACCCAATGGATGCCGGAAGTCTGGCTCAATGTCGGCATCGACTACG
ACCGCGACGGTCGCGTCTCGCCGTTCGGCCGCCCCGACGACGCCCTCGGCTCCAGCGCAAAATATCTGGTCAATCGCGGC
AAATATCGCCGCGGCGAGCATTGGGGCTACGAGGTCCGCGGGCCCGGCGGCGCGATCAGCGGCAGCCGTTCGTATCTCGC
GTGGTCGCGCTCCGGCGTCAGCCGCGTCGACGGCGAGCCGTTTCCCGATCCGCAGGCGATGGCGCAGATGTGGGTGCCGG
TCGCGGGCGGTCCGCAGTTTCTGCTGGGACCGAATTTCTACGCGGTGCGCAGCTACAACCCGTCGATGAACTACGCACTC
GCGATCTGCCATCTCGGCGACCGCATCCTCGGCGCCGGCCCGTTCGCGAATCCGTTCCCGGGCTCGGAGCGGGCGATGAC
GCTGGCCGAAGTGCAGGAGATGCAGACCCGGCTGACCCGCGCCGGCTTCGACACCGGCGGCACCGACGGCCGCGTCGGCA
ACGATACGATGAAAGCGGTGCGCGATTATCAGGCGCGCGCCGGCATGCAGCCGGCCGACGGCTATGGCGGCCTCAAGCTG
CTTGCGCGGCTGCGGCAGGGCCCGTAA

Upstream 100 bases:

>100_bases
CACGCCCTGATCGTCGCCGCGGCTGCCGTTCTGCCCTGCCCGAGATCAATTGCTCGCGGGCCTCTGCCCCGCGCCGGCTT
CAGCCGCTACACTGCGCCGT

Downstream 100 bases:

>100_bases
GTCATTTCCGCAATGCGACATCATTGGACCTGGCACCGGGTGAAGGCGCTCGCGCACGAGGAGTGGCGCGCGCTGGTCAC
CATCAATCCGAGCGACCGGC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MASFDSRFTPTRRRLLQTALGAAVMWPAIARAAPAGFDAWRDGFRSRALAKGITPQTWDRAMARLEPDMSVFKNFQKQPE
FHEQLWQYINRRVSDWRITNGRLALQKTEPLFARIERDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPSLAALAWNE
PRRKAYWETELINALRIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGIDYDRDGRVSPFGRPDDALGSSAKYLVNRG
KYRRGEHWGYEVRGPGGAISGSRSYLAWSRSGVSRVDGEPFPDPQAMAQMWVPVAGGPQFLLGPNFYAVRSYNPSMNYAL
AICHLGDRILGAGPFANPFPGSERAMTLAEVQEMQTRLTRAGFDTGGTDGRVGNDTMKAVRDYQARAGMQPADGYGGLKL
LARLRQGP

Sequences:

>Translated_408_residues
MASFDSRFTPTRRRLLQTALGAAVMWPAIARAAPAGFDAWRDGFRSRALAKGITPQTWDRAMARLEPDMSVFKNFQKQPE
FHEQLWQYINRRVSDWRITNGRLALQKTEPLFARIERDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPSLAALAWNE
PRRKAYWETELINALRIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGIDYDRDGRVSPFGRPDDALGSSAKYLVNRG
KYRRGEHWGYEVRGPGGAISGSRSYLAWSRSGVSRVDGEPFPDPQAMAQMWVPVAGGPQFLLGPNFYAVRSYNPSMNYAL
AICHLGDRILGAGPFANPFPGSERAMTLAEVQEMQTRLTRAGFDTGGTDGRVGNDTMKAVRDYQARAGMQPADGYGGLKL
LARLRQGP
>Mature_407_residues
ASFDSRFTPTRRRLLQTALGAAVMWPAIARAAPAGFDAWRDGFRSRALAKGITPQTWDRAMARLEPDMSVFKNFQKQPEF
HEQLWQYINRRVSDWRITNGRLALQKTEPLFARIERDFGVERGTLLALWGVESAYGDPLVQQNHMRPVFPSLAALAWNEP
RRKAYWETELINALRIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGIDYDRDGRVSPFGRPDDALGSSAKYLVNRGK
YRRGEHWGYEVRGPGGAISGSRSYLAWSRSGVSRVDGEPFPDPQAMAQMWVPVAGGPQFLLGPNFYAVRSYNPSMNYALA
ICHLGDRILGAGPFANPFPGSERAMTLAEVQEMQTRLTRAGFDTGGTDGRVGNDTMKAVRDYQARAGMQPADGYGGLKLL
ARLRQGP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=245, Percent_Identity=27.3469387755102, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45702; Mature: 45571

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFDSRFTPTRRRLLQTALGAAVMWPAIARAAPAGFDAWRDGFRSRALAKGITPQTWDR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AMARLEPDMSVFKNFQKQPEFHEQLWQYINRRVSDWRITNGRLALQKTEPLFARIERDFG
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCHHHHHHHHHHC
VERGTLLALWGVESAYGDPLVQQNHMRPVFPSLAALAWNEPRRKAYWETELINALRIVDR
CCCCCEEEEECCCHHHCCCCHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GWSTPEEMRGSWAGAMGHTQWMPEVWLNVGIDYDRDGRVSPFGRPDDALGSSAKYLVNRG
CCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHCC
KYRRGEHWGYEVRGPGGAISGSRSYLAWSRSGVSRVDGEPFPDPQAMAQMWVPVAGGPQF
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCE
LLGPNFYAVRSYNPSMNYALAICHLGDRILGAGPFANPFPGSERAMTLAEVQEMQTRLTR
EECCCEEEEEECCCCCCCEEEEEHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGFDTGGTDGRVGNDTMKAVRDYQARAGMQPADGYGGLKLLARLRQGP
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ASFDSRFTPTRRRLLQTALGAAVMWPAIARAAPAGFDAWRDGFRSRALAKGITPQTWDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHH
AMARLEPDMSVFKNFQKQPEFHEQLWQYINRRVSDWRITNGRLALQKTEPLFARIERDFG
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCEEEEECCCHHHHHHHHHHC
VERGTLLALWGVESAYGDPLVQQNHMRPVFPSLAALAWNEPRRKAYWETELINALRIVDR
CCCCCEEEEECCCHHHCCCCHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GWSTPEEMRGSWAGAMGHTQWMPEVWLNVGIDYDRDGRVSPFGRPDDALGSSAKYLVNRG
CCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHCC
KYRRGEHWGYEVRGPGGAISGSRSYLAWSRSGVSRVDGEPFPDPQAMAQMWVPVAGGPQF
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCE
LLGPNFYAVRSYNPSMNYALAICHLGDRILGAGPFANPFPGSERAMTLAEVQEMQTRLTR
EECCCEEEEEECCCCCCCEEEEEHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGFDTGGTDGRVGNDTMKAVRDYQARAGMQPADGYGGLKLLARLRQGP
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]