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The map label for this gene is gloA [H]

Identifier: 86750057

GI number: 86750057

Start: 3352876

End: 3353325

Strand: Direct

Name: gloA [H]

Synonym: RPB_2940

Alternate gene names: 86750057

Gene position: 3352876-3353325 (Clockwise)

Preceding gene: 86750053

Following gene: 86750067

Centisome position: 62.89

GC content: 62.67

Gene sequence:

>450_bases
ATGCGTTTTCTGCACACCATGCTGCGCGTCCGCGATCTCGACACCGCGCTGCAATTCTTCAGCGGCGCGCTCGGCCTTCA
GGAAGTCCGACGCATCGACAACGACAAGGGACGATTCACGCTGCTGTTTCTGTGCGCGCCAGAAGACGTGGAACTGGTCA
GGCAATCGCCCGGGCGCCCGGCGCCGACGATCGAACTCACGCACAATTGGGACGCGGAATCGTACGGCGAGGATCGCTTC
TTCGGCCATCTCGCTTACGAGGTCGACGACATCTACGCCACGTGCGAGCGGCTGATGAAGATGGGCATCACCATCAACAG
GCCGCCGCGCGACGGTCAGATGGCGTTCGTGCGTTCTCCCGATCTGCATTCCGTCGAGTTGCTGCAGAAGGGCGATGCGT
TGCCGCCGCAGGAGCCGTGGGCATCGATGCCCAACACCGGCCACTGGTAA

Upstream 100 bases:

>100_bases
CCATCGCCCGCGCGGCCGAGCGCGCTCCGCCAGCGCTTTCCAAAGCCGCCGTTTCGTCGCAAGATCGCGCTCTCGCTCGC
CTCACGGAGACACCACCAAC

Downstream 100 bases:

>100_bases
TCGCGGCGGCCCGCAGCGCGGCGAACTAACACGCATCGCATTCACATCGATGCGTTTTGTTCCGGATGATCGGGCTTCGA
AATCAGTCGGCTTCCAGGAA

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MRFLHTMLRVRDLDTALQFFSGALGLQEVRRIDNDKGRFTLLFLCAPEDVELVRQSPGRPAPTIELTHNWDAESYGEDRF
FGHLAYEVDDIYATCERLMKMGITINRPPRDGQMAFVRSPDLHSVELLQKGDALPPQEPWASMPNTGHW

Sequences:

>Translated_149_residues
MRFLHTMLRVRDLDTALQFFSGALGLQEVRRIDNDKGRFTLLFLCAPEDVELVRQSPGRPAPTIELTHNWDAESYGEDRF
FGHLAYEVDDIYATCERLMKMGITINRPPRDGQMAFVRSPDLHSVELLQKGDALPPQEPWASMPNTGHW
>Mature_149_residues
MRFLHTMLRVRDLDTALQFFSGALGLQEVRRIDNDKGRFTLLFLCAPEDVELVRQSPGRPAPTIELTHNWDAESYGEDRF
FGHLAYEVDDIYATCERLMKMGITINRPPRDGQMAFVRSPDLHSVELLQKGDALPPQEPWASMPNTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=32.1678321678322, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787940, Length=139, Percent_Identity=35.2517985611511, Blast_Score=87, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=31.6901408450704, Blast_Score=74, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=32.4137931034483, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 17100; Mature: 17100

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTMLRVRDLDTALQFFSGALGLQEVRRIDNDKGRFTLLFLCAPEDVELVRQSPGRP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCC
APTIELTHNWDAESYGEDRFFGHLAYEVDDIYATCERLMKMGITINRPPRDGQMAFVRSP
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECC
DLHSVELLQKGDALPPQEPWASMPNTGHW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRFLHTMLRVRDLDTALQFFSGALGLQEVRRIDNDKGRFTLLFLCAPEDVELVRQSPGRP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCC
APTIELTHNWDAESYGEDRFFGHLAYEVDDIYATCERLMKMGITINRPPRDGQMAFVRSP
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECC
DLHSVELLQKGDALPPQEPWASMPNTGHW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]