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The map label for this gene is rimO

Identifier: 86749957

GI number: 86749957

Start: 3233347

End: 3234672

Strand: Direct

Name: rimO

Synonym: RPB_2840

Alternate gene names: 86749957

Gene position: 3233347-3234672 (Clockwise)

Preceding gene: 86749956

Following gene: 86749960

Centisome position: 60.64

GC content: 66.21

Gene sequence:

>1326_bases
ATGCAGCAGGCGGCCGCGCCAAAAATCAGTTTTGTCTCGCTGGGATGCCCCAAGGCGCTGGTGGATTCCGAGCGGATCAT
CACCCGGCTGCGCGCCGAGGGCTACGAGCTCGCGCGCCAGCATGACGGCGCCGATCTGGTCATCGTCAACACCTGCGGCT
TCCTCGACAGCGCCAAGCAGGAATCGCTCGCCGCGATCGGCGAAGCGATGGCTGCGAACGGCAAGGTGATCGTCACCGGC
TGCATGGGCGCCGAGCCGGAGCAGATCGAGGCCGCCTACCCCGGCGTGCTGTCGATCACCGGCCCGCAGCAATATGAGAG
CGTGCTCGACGCGGTGCATCGCGCCAAGCCGGCACTGCACAACCCGCATCTCGATCTCGTACCCGAGCAGGGCATCCGGC
TGACGCCGCGGCATTACGCGTATTTGAAGATTTCAGAAGGCTGCAACAACCGCTGCAGCTTCTGCATCATCCCGAAGCTG
CGCGGCGATCTGGCGTCGCGCTCCGCCGCCGACGTGCTGCGCGAGGCCGAGAAGCTGGTCGCCGCCGGCGTCAAGGAACT
GCTGGTGATCTCGCAGGACACTTCGGCCTATGGTGTCGATCTGAAATACGCCGAGAGCCCTTGGAAGGACCGCAGCGTCC
GCGCCAAATTCATCGATCTGGCGCGCGAACTCGGCGAGCTCGGCGCCTGGGTCCGGCTGCACTACGTCTACCCCTATCCG
CATGTCGACGAAGTGATCGGCCTGATGACCGAGGGCAAGGTGCTGCCCTATCTCGACATCCCGTTCCAGCACGCCAGCCC
CGACGTGCTCAAGCTGATGAAGCGCCCGGCCGCGCAGGACAAGACGCTGGAGCGCATCAAGCGCTGGCGCGCGCAATGCC
CGGACCTGGCGCTCAGATCGACCTTCATCGTCGGCTTCCCCGGCGAGACCGAGCGCGACTTCGAATTCCTGCTCGACTGG
CTCGACGAAGCCGAGATCGATCGCCTCGGCGCGTTCAAATACGAGCCGGTCGCCGGCGCGCCGTCAAATGCGCTCGAAGG
TCAGGTGCCGGACGAGGTCAAGCAGGAGCGCTGGAATCGGCTGATGGCGCGGCAGCAGGCGATCTCGGCGCGGCGGCTGA
AGCGCAAGGTCGGCACGCGGCAGCAGATCATCATCGACGAGATCGGCCCGACCGTCGCCAAAGGCCGCTCCAAGGCCGAT
GCGCCGGAGATCGATGGCAGCGTGTATCTGACCAGCCGCCGCCCGCTCCGCGTCGGCGAAATCGTCACCGCCAAGATCGA
CCGCGCCGACGCCTACGATCTGCACGGGACGGTGGCGGGGTTCTGA

Upstream 100 bases:

>100_bases
GACTGGACGGAAATTGTTCTCGGTCCATCCCGCCGGAACCGCCTTGTCAGCGCCGCCGAAGGGGTTTAGCTCGGGGCGAT
CAATCACACAGTTGGAACCG

Downstream 100 bases:

>100_bases
GCGTCTCGGCCTCAACGTAATTCACGTCATCCTGAGGCGCCGTCGCGCAGCGACGGCCTCGAAGGATGGGCCGCGCGTGC
GTTTACCAGCATCCTTCGAG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQQAAAPKISFVSLGCPKALVDSERIITRLRAEGYELARQHDGADLVIVNTCGFLDSAKQESLAAIGEAMAANGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRAKPALHNPHLDLVPEQGIRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLASRSAADVLREAEKLVAAGVKELLVISQDTSAYGVDLKYAESPWKDRSVRAKFIDLARELGELGAWVRLHYVYPYP
HVDEVIGLMTEGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLERIKRWRAQCPDLALRSTFIVGFPGETERDFEFLLDW
LDEAEIDRLGAFKYEPVAGAPSNALEGQVPDEVKQERWNRLMARQQAISARRLKRKVGTRQQIIIDEIGPTVAKGRSKAD
APEIDGSVYLTSRRPLRVGEIVTAKIDRADAYDLHGTVAGF

Sequences:

>Translated_441_residues
MQQAAAPKISFVSLGCPKALVDSERIITRLRAEGYELARQHDGADLVIVNTCGFLDSAKQESLAAIGEAMAANGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRAKPALHNPHLDLVPEQGIRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLASRSAADVLREAEKLVAAGVKELLVISQDTSAYGVDLKYAESPWKDRSVRAKFIDLARELGELGAWVRLHYVYPYP
HVDEVIGLMTEGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLERIKRWRAQCPDLALRSTFIVGFPGETERDFEFLLDW
LDEAEIDRLGAFKYEPVAGAPSNALEGQVPDEVKQERWNRLMARQQAISARRLKRKVGTRQQIIIDEIGPTVAKGRSKAD
APEIDGSVYLTSRRPLRVGEIVTAKIDRADAYDLHGTVAGF
>Mature_441_residues
MQQAAAPKISFVSLGCPKALVDSERIITRLRAEGYELARQHDGADLVIVNTCGFLDSAKQESLAAIGEAMAANGKVIVTG
CMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRAKPALHNPHLDLVPEQGIRLTPRHYAYLKISEGCNNRCSFCIIPKL
RGDLASRSAADVLREAEKLVAAGVKELLVISQDTSAYGVDLKYAESPWKDRSVRAKFIDLARELGELGAWVRLHYVYPYP
HVDEVIGLMTEGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLERIKRWRAQCPDLALRSTFIVGFPGETERDFEFLLDW
LDEAEIDRLGAFKYEPVAGAPSNALEGQVPDEVKQERWNRLMARQQAISARRLKRKVGTRQQIIIDEIGPTVAKGRSKAD
APEIDGSVYLTSRRPLRVGEIVTAKIDRADAYDLHGTVAGF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=399, Percent_Identity=28.8220551378446, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=25.1046025104602, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=24.8953974895398, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787057, Length=432, Percent_Identity=64.1203703703704, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1786882, Length=401, Percent_Identity=28.6783042394015, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=481, Percent_Identity=23.7006237006237, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=28.90625, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=25.7322175732218, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922432, Length=375, Percent_Identity=26.4, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_RHOP2 (Q2IW68)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486453.1
- ProteinModelPortal:   Q2IW68
- STRING:   Q2IW68
- GeneID:   3910633
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2840
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   RPAL316058:RPB_2840-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48789; Mature: 48789

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQAAAPKISFVSLGCPKALVDSERIITRLRAEGYELARQHDGADLVIVNTCGFLDSAKQ
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
ESLAAIGEAMAANGKVIVTGCMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRAKPALH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
NPHLDLVPEQGIRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLASRSAADVLREAEKLV
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECHHHCHHHHHHHHHHHHHHHHHH
AAGVKELLVISQDTSAYGVDLKYAESPWKDRSVRAKFIDLARELGELGAWVRLHYVYPYP
HHCHHHHEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCC
HVDEVIGLMTEGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLERIKRWRAQCPDLALRS
CHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECC
TFIVGFPGETERDFEFLLDWLDEAEIDRLGAFKYEPVAGAPSNALEGQVPDEVKQERWNR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHH
LMARQQAISARRLKRKVGTRQQIIIDEIGPTVAKGRSKADAPEIDGSVYLTSRRPLRVGE
HHHHHHHHHHHHHHHHHCCCCEEEHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCEEECC
IVTAKIDRADAYDLHGTVAGF
EEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQAAAPKISFVSLGCPKALVDSERIITRLRAEGYELARQHDGADLVIVNTCGFLDSAKQ
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
ESLAAIGEAMAANGKVIVTGCMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRAKPALH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
NPHLDLVPEQGIRLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLASRSAADVLREAEKLV
CCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECHHHCHHHHHHHHHHHHHHHHHH
AAGVKELLVISQDTSAYGVDLKYAESPWKDRSVRAKFIDLARELGELGAWVRLHYVYPYP
HHCHHHHEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCC
HVDEVIGLMTEGKVLPYLDIPFQHASPDVLKLMKRPAAQDKTLERIKRWRAQCPDLALRS
CHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECC
TFIVGFPGETERDFEFLLDWLDEAEIDRLGAFKYEPVAGAPSNALEGQVPDEVKQERWNR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHH
LMARQQAISARRLKRKVGTRQQIIIDEIGPTVAKGRSKADAPEIDGSVYLTSRRPLRVGE
HHHHHHHHHHHHHHHHHCCCCEEEHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCEEECC
IVTAKIDRADAYDLHGTVAGF
EEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA