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The map label for this gene is xthA [H]

Identifier: 86749875

GI number: 86749875

Start: 3145271

End: 3146101

Strand: Direct

Name: xthA [H]

Synonym: RPB_2758

Alternate gene names: 86749875

Gene position: 3145271-3146101 (Clockwise)

Preceding gene: 86749871

Following gene: 86749876

Centisome position: 58.99

GC content: 62.45

Gene sequence:

>831_bases
ATGGCACCAGCCCGGATTTTTGAACCGACGGATAACGACATGCGGATCGCAACCTGGAACGTAAACTCTGTCAGGCAGCG
ACTGGATCACCTGGTCAGTTGGCTGAACGAGTGCGCGCCGGACGTGGTGTGCCTGCAGGAGATCAAGTGCGTCGACGAGG
CGTTTCCGCGCGAGGCGATCGAAGCGCTCGGCTACAATGTCGTCACCCACGGCCAGAAGACGTTCAATGGCGTCGCATTG
CTGTCGAAATATCCGCTGGAGGAAGCCACCCCTCGCCTCGCCGGCGACGACGATGATACTCACGCCCGATTCCTCGAAGG
CGTTGTGTCGCTGAAGCGCGGCGTGGTCCGTGTCGCCTGCCTCTATCTGCCTAACGGGAATCCGGTCGAGAGTGAAAAAT
ATCCCTACAAGCTACGCTGGATGTCGCGGCTTCTCGAGTACACGCAGCAGCGCCTGAAAACCGAGGAGCCGCTGATCCTG
GCCGGCGACTTCAACGTGATCCCGCAACCCATCGACGTCCACAACCCGGCCGGCTGGGTCGATGATGCATTGTTCCGGCC
GCAGACGCGGGAGAGTTTCCAGGCTCTGCTCGGCCTCGGACTCACCGATGCGCTGCGCGCCTCCACCGACGCGCCGGGCC
AATACACATTCTGGGACTACCAGGCCGGAGCCTGGCAGAAGAATTGGGGACTGCGGATCGACCACCTGCTGCTGTCGCCG
CAGGCTACCGACCGGCTCCGCAATGTCGGCATCGACGCTTACGTGCGGAACTGGGAGAAGCCGTCCGACCACGTGCCGGT
GTGGGCGGATTTCGATCTGGATGCCGCCTGA

Upstream 100 bases:

>100_bases
TTGATCTTGCCACGCCCGACGGCATCCACCGCCCGCGCGCTCCCGAGGCGTCATTACGCCCGATTTTTCTGAGGCTTACC
ACAGCCCCCGCAACCATGGT

Downstream 100 bases:

>100_bases
GGCTTCGGGGCTTGATCCAAGCAAAGCTCCGTAGCGAAATTCGGTCTGGCCAAGCCCAAAACCGACCGTCGCCGATGTTT
CGTGTGCACCTGAGCGATCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAPARIFEPTDNDMRIATWNVNSVRQRLDHLVSWLNECAPDVVCLQEIKCVDEAFPREAIEALGYNVVTHGQKTFNGVAL
LSKYPLEEATPRLAGDDDDTHARFLEGVVSLKRGVVRVACLYLPNGNPVESEKYPYKLRWMSRLLEYTQQRLKTEEPLIL
AGDFNVIPQPIDVHNPAGWVDDALFRPQTRESFQALLGLGLTDALRASTDAPGQYTFWDYQAGAWQKNWGLRIDHLLLSP
QATDRLRNVGIDAYVRNWEKPSDHVPVWADFDLDAA

Sequences:

>Translated_276_residues
MAPARIFEPTDNDMRIATWNVNSVRQRLDHLVSWLNECAPDVVCLQEIKCVDEAFPREAIEALGYNVVTHGQKTFNGVAL
LSKYPLEEATPRLAGDDDDTHARFLEGVVSLKRGVVRVACLYLPNGNPVESEKYPYKLRWMSRLLEYTQQRLKTEEPLIL
AGDFNVIPQPIDVHNPAGWVDDALFRPQTRESFQALLGLGLTDALRASTDAPGQYTFWDYQAGAWQKNWGLRIDHLLLSP
QATDRLRNVGIDAYVRNWEKPSDHVPVWADFDLDAA
>Mature_275_residues
APARIFEPTDNDMRIATWNVNSVRQRLDHLVSWLNECAPDVVCLQEIKCVDEAFPREAIEALGYNVVTHGQKTFNGVALL
SKYPLEEATPRLAGDDDDTHARFLEGVVSLKRGVVRVACLYLPNGNPVESEKYPYKLRWMSRLLEYTQQRLKTEEPLILA
GDFNVIPQPIDVHNPAGWVDDALFRPQTRESFQALLGLGLTDALRASTDAPGQYTFWDYQAGAWQKNWGLRIDHLLLSPQ
ATDRLRNVGIDAYVRNWEKPSDHVPVWADFDLDAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=261, Percent_Identity=31.8007662835249, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI18375503, Length=261, Percent_Identity=31.8007662835249, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI18375501, Length=261, Percent_Identity=31.8007662835249, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=34.9624060150376, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=29.7397769516729, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31316; Mature: 31184

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPARIFEPTDNDMRIATWNVNSVRQRLDHLVSWLNECAPDVVCLQEIKCVDEAFPREAI
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EALGYNVVTHGQKTFNGVALLSKYPLEEATPRLAGDDDDTHARFLEGVVSLKRGVVRVAC
HHHCCHHEECCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEE
LYLPNGNPVESEKYPYKLRWMSRLLEYTQQRLKTEEPLILAGDFNVIPQPIDVHNPAGWV
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCH
DDALFRPQTRESFQALLGLGLTDALRASTDAPGQYTFWDYQAGAWQKNWGLRIDHLLLSP
HHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEHEEECC
QATDRLRNVGIDAYVRNWEKPSDHVPVWADFDLDAA
HHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
APARIFEPTDNDMRIATWNVNSVRQRLDHLVSWLNECAPDVVCLQEIKCVDEAFPREAI
CCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EALGYNVVTHGQKTFNGVALLSKYPLEEATPRLAGDDDDTHARFLEGVVSLKRGVVRVAC
HHHCCHHEECCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEE
LYLPNGNPVESEKYPYKLRWMSRLLEYTQQRLKTEEPLILAGDFNVIPQPIDVHNPAGWV
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCH
DDALFRPQTRESFQALLGLGLTDALRASTDAPGQYTFWDYQAGAWQKNWGLRIDHLLLSP
HHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEHEEECC
QATDRLRNVGIDAYVRNWEKPSDHVPVWADFDLDAA
HHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]