Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 86749876

GI number: 86749876

Start: 3146779

End: 3148131

Strand: Direct

Name: livK [C]

Synonym: RPB_2759

Alternate gene names: 86749876

Gene position: 3146779-3148131 (Clockwise)

Preceding gene: 86749875

Following gene: 86749877

Centisome position: 59.02

GC content: 63.19

Gene sequence:

>1353_bases
ATGGCCAACGACAAGCTCATCATCAATCGTAGAACTGCACTCAAGACCGGGCTTGCCGGCGCGGCCGCGCTGGCAACGCC
GACGTTCTTCATCCGCGACGCCTGGGCCGACGACTTCTGCAACATGCCCAAGGGCAAGGAAGTCACGTTCGGCTTCAACG
TGCCGCAGTCCGGCGCCTATGCCGATGAAGGCATGGATGAACTCAAGGCCTATCAGCTCGCCGTCAAGCACCTCAATGGT
GAGGGCGATGGCGGCATGCTCAAGACCATGAAGCCGCTGGCGCTGAAGGGCAACGGCGTGCTCGGCAAGAAGGTCGCCTA
CGTCTCCGGCGACACCCAGACGAAGGCCGATCAGGCGCGCGCCACCGCCAAGCGCATGATCGAGAAGGACGGCGCGATCA
TGATCACCGGCGGCTCGTCGTCGGCCGAAGCGGTCGCGGTGCAATCGCTGTGTCAGGACGTCGGCGTGATCTTCATGGCC
GGCCTCACCCACTCCAACGACACCACCGGCAAGGACAAGCGCGCCTACGGCTTCCGCCACTTCTTCAACGCCTATATGTC
GGGCGTGGCGCTTGGACCGGTGCTCTCCGAAGCCTATGGCAAGGACCGCGCCGCCTATCACCTGACCGCCGACTACACCT
GGGGCTGGACCCAGGAAGAGTCGATGAAGGATGCGACCGAAAAGCAGGGCTGGAAGACGGTCAAGGCCGTCCGCACCCCG
CTCGGCGCTGCCGACTTCTCGCAGTACATCACCCCGGTGCTGAATTCCGGCGCCGACGTGCTGATCCTGAACCACTACGG
CAAGGACATGATCAACTCCTTGACCCAGGCGGTGCAGTTCGGCCTGCGCGACAAGATGGCGAACGGCAAGAAGTTTGAAA
TCGTCGTTCCGCTGTTCTCTGAACTGATGGCGCAAGGCGCCGGCGATGCGATCAAGGGCATCTACGGCACCGCCAATTGG
GACTGGAAGCTGGAAGACGCAGCGACCAGCGCCTTCACCAAGTCGTTCGGTGCGGCCTATGGCACGCCGCCGTCGCAGGC
CGCCCAGACCTGCTACGTTCAGGCGATCCTCTACGCCGACGCCTGCGAGCGTGCCGGCAGTTTCAACCCGTCTGCCGTGA
TCAAGGCGCTGGAAGGCTTCGAGTTCGACGGCATGGGCAACGGCAAGACGCTGTACCGCGCCGCCGACCATCAGTGCTTC
AAGGACGTGCTGGTGGTGCAGGGCAAGGACAAGCCGAAGGACAAGTTCGACCTGCTCGAGGTCCGGAAGATCGTGCCGGC
GTCTCAGGTCACCTATGACCCGAGCATCTTCGGCGGCGAACTCGGCTCCAAGGACGCCAAGAAGTGCGGATAA

Upstream 100 bases:

>100_bases
ACATGCCTCCAGAAGCGACCAAAGCTCTGGCCGAACGATCTCGCATGTGTGGGAGCGACGGTAACAAGAAGCGCTGATGT
TGAAACGCCGAGGAGGAAAC

Downstream 100 bases:

>100_bases
TCTGACCCTGTCGGATTTGCCGCAAGCAATTCGGTAGGCCTGGACTCCTATTCCGGTCGGTTGCGTTCTGGCGACGCAGC
CGGCCGGCTGTGTCTTGCGA

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: UreA/Short-Chain Amide ABC Transporter; ABC-Type Transporter Periplasmic Component; Branched-Chain Amino Acid ABC Transporter; ABC-Type Transporter Periplasmic; Tat Pathway Signal Sequence Domain-Containing Protein; ABC Transporter; Tat Pathway Signal Sequence Domain Protein; Amino Acid ABC Transporter Periplasmic Protein; Inner-Membrane Translocator; ABC Transporter Periplasmic Protein

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MANDKLIINRRTALKTGLAGAAALATPTFFIRDAWADDFCNMPKGKEVTFGFNVPQSGAYADEGMDELKAYQLAVKHLNG
EGDGGMLKTMKPLALKGNGVLGKKVAYVSGDTQTKADQARATAKRMIEKDGAIMITGGSSSAEAVAVQSLCQDVGVIFMA
GLTHSNDTTGKDKRAYGFRHFFNAYMSGVALGPVLSEAYGKDRAAYHLTADYTWGWTQEESMKDATEKQGWKTVKAVRTP
LGAADFSQYITPVLNSGADVLILNHYGKDMINSLTQAVQFGLRDKMANGKKFEIVVPLFSELMAQGAGDAIKGIYGTANW
DWKLEDAATSAFTKSFGAAYGTPPSQAAQTCYVQAILYADACERAGSFNPSAVIKALEGFEFDGMGNGKTLYRAADHQCF
KDVLVVQGKDKPKDKFDLLEVRKIVPASQVTYDPSIFGGELGSKDAKKCG

Sequences:

>Translated_450_residues
MANDKLIINRRTALKTGLAGAAALATPTFFIRDAWADDFCNMPKGKEVTFGFNVPQSGAYADEGMDELKAYQLAVKHLNG
EGDGGMLKTMKPLALKGNGVLGKKVAYVSGDTQTKADQARATAKRMIEKDGAIMITGGSSSAEAVAVQSLCQDVGVIFMA
GLTHSNDTTGKDKRAYGFRHFFNAYMSGVALGPVLSEAYGKDRAAYHLTADYTWGWTQEESMKDATEKQGWKTVKAVRTP
LGAADFSQYITPVLNSGADVLILNHYGKDMINSLTQAVQFGLRDKMANGKKFEIVVPLFSELMAQGAGDAIKGIYGTANW
DWKLEDAATSAFTKSFGAAYGTPPSQAAQTCYVQAILYADACERAGSFNPSAVIKALEGFEFDGMGNGKTLYRAADHQCF
KDVLVVQGKDKPKDKFDLLEVRKIVPASQVTYDPSIFGGELGSKDAKKCG
>Mature_449_residues
ANDKLIINRRTALKTGLAGAAALATPTFFIRDAWADDFCNMPKGKEVTFGFNVPQSGAYADEGMDELKAYQLAVKHLNGE
GDGGMLKTMKPLALKGNGVLGKKVAYVSGDTQTKADQARATAKRMIEKDGAIMITGGSSSAEAVAVQSLCQDVGVIFMAG
LTHSNDTTGKDKRAYGFRHFFNAYMSGVALGPVLSEAYGKDRAAYHLTADYTWGWTQEESMKDATEKQGWKTVKAVRTPL
GAADFSQYITPVLNSGADVLILNHYGKDMINSLTQAVQFGLRDKMANGKKFEIVVPLFSELMAQGAGDAIKGIYGTANWD
WKLEDAATSAFTKSFGAAYGTPPSQAAQTCYVQAILYADACERAGSFNPSAVIKALEGFEFDGMGNGKTLYRAADHQCFK
DVLVVQGKDKPKDKFDLLEVRKIVPASQVTYDPSIFGGELGSKDAKKCG

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48372; Mature: 48241

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDKLIINRRTALKTGLAGAAALATPTFFIRDAWADDFCNMPKGKEVTFGFNVPQSGAY
CCCCCEEEECHHHHHHCCCCHHHHHCCHHEEECCCHHHHHCCCCCCEEEEECCCCCCCCC
ADEGMDELKAYQLAVKHLNGEGDGGMLKTMKPLALKGNGVLGKKVAYVSGDTQTKADQAR
HHCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCEEEEECCCCCHHHHHHH
ATAKRMIEKDGAIMITGGSSSAEAVAVQSLCQDVGVIFMAGLTHSNDTTGKDKRAYGFRH
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHH
FFNAYMSGVALGPVLSEAYGKDRAAYHLTADYTWGWTQEESMKDATEKQGWKTVKAVRTP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LGAADFSQYITPVLNSGADVLILNHYGKDMINSLTQAVQFGLRDKMANGKKFEIVVPLFS
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
ELMAQGAGDAIKGIYGTANWDWKLEDAATSAFTKSFGAAYGTPPSQAAQTCYVQAILYAD
HHHHCCCCCHHHCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ACERAGSFNPSAVIKALEGFEFDGMGNGKTLYRAADHQCFKDVLVVQGKDKPKDKFDLLE
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHEECCCCCCCHHHHHHH
VRKIVPASQVTYDPSIFGGELGSKDAKKCG
HHHHCCCCCEECCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure 
ANDKLIINRRTALKTGLAGAAALATPTFFIRDAWADDFCNMPKGKEVTFGFNVPQSGAY
CCCCEEEECHHHHHHCCCCHHHHHCCHHEEECCCHHHHHCCCCCCEEEEECCCCCCCCC
ADEGMDELKAYQLAVKHLNGEGDGGMLKTMKPLALKGNGVLGKKVAYVSGDTQTKADQAR
HHCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEECCCCCCCCEEEEECCCCCHHHHHHH
ATAKRMIEKDGAIMITGGSSSAEAVAVQSLCQDVGVIFMAGLTHSNDTTGKDKRAYGFRH
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHH
FFNAYMSGVALGPVLSEAYGKDRAAYHLTADYTWGWTQEESMKDATEKQGWKTVKAVRTP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LGAADFSQYITPVLNSGADVLILNHYGKDMINSLTQAVQFGLRDKMANGKKFEIVVPLFS
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
ELMAQGAGDAIKGIYGTANWDWKLEDAATSAFTKSFGAAYGTPPSQAAQTCYVQAILYAD
HHHHCCCCCHHHCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ACERAGSFNPSAVIKALEGFEFDGMGNGKTLYRAADHQCFKDVLVVQGKDKPKDKFDLLE
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHEECCCCCCCHHHHHHH
VRKIVPASQVTYDPSIFGGELGSKDAKKCG
HHHHCCCCCEECCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA