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The map label for this gene is nuoI [H]

Identifier: 86749700

GI number: 86749700

Start: 2963762

End: 2964250

Strand: Direct

Name: nuoI [H]

Synonym: RPB_2582

Alternate gene names: 86749700

Gene position: 2963762-2964250 (Clockwise)

Preceding gene: 86749699

Following gene: 86749701

Centisome position: 55.59

GC content: 62.78

Gene sequence:

>489_bases
ATGAATATCAACGCAACCGCCCGTTCGCTGCTGCTGACGGAATTCGTATCGGCGTTCTTCCTCGCCATGCGCTATTTCTT
CAAGCCGAAGCCGACGATCAATTATCCTTTCGAGAAAAATCCGATCTCGCCCCGCTTCCGCGGCGAGCACGCGCTGCGCC
GCTATCCCAACGGCGAGGAGCGCTGCATCGCCTGCAAGCTGTGCGAGGCGATCTGCCCGGCGCAGGCGATCACCATCGAG
GCCGGTCCGCGGCGCAACGACGGCACCCGCCGCACCGTGCGCTACGACATCGACATGGTGAAGTGCATCTATTGCGGGCT
GTGCCAGGAGGCCTGCCCGGTCGACGCGATCGTCGAGGGGCCGAATTTCGAATTCGCCACCGAGACCCGCGAAGAGCTGT
ATTACGACAAGGCTCGGCTGCTCGCGAACGGTGATCGTTGGGAGCGCGAGATCGCCAAGTCGATCGAACTCGACGCGCCG
TACCGGTGA

Upstream 100 bases:

>100_bases
CGGCTCGGCTGGAAGGTGTTCCTGCCGCTGTCGCTGGCGATGGTGGTGATCGTCGCCGCCGTCCTGCAATTCGCGGGGCT
CGCGCCGAAATGAGGCCGTC

Downstream 100 bases:

>100_bases
GCTGACGCCATGTCCATGCCTGTCCTGTTCTTCTATCTCTACGCCGGGGCCGCGATCGCCGCCGCGGTGATGGTGGTGTT
CGCGCGCAACCCGGTGCACT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNINATARSLLLTEFVSAFFLAMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKARLLANGDRWEREIAKSIELDAP
YR

Sequences:

>Translated_162_residues
MNINATARSLLLTEFVSAFFLAMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKARLLANGDRWEREIAKSIELDAP
YR
>Mature_162_residues
MNINATARSLLLTEFVSAFFLAMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKARLLANGDRWEREIAKSIELDAP
YR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=156, Percent_Identity=73.0769230769231, Blast_Score=239, Evalue=6e-64,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=38.7323943661972, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789075, Length=127, Percent_Identity=28.3464566929134, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=161, Percent_Identity=66.4596273291926, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17864306, Length=156, Percent_Identity=69.8717948717949, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18715; Mature: 18715

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINATARSLLLTEFVSAFFLAMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCH
RCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEG
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEECC
PNFEFATETREELYYDKARLLANGDRWEREIAKSIELDAPYR
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNINATARSLLLTEFVSAFFLAMRYFFKPKPTINYPFEKNPISPRFRGEHALRRYPNGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCH
RCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGLCQEACPVDAIVEG
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEECC
PNFEFATETREELYYDKARLLANGDRWEREIAKSIELDAPYR
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]