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The map label for this gene is nuoB2 [H]

Identifier: 86749691

GI number: 86749691

Start: 2954839

End: 2955453

Strand: Direct

Name: nuoB2 [H]

Synonym: RPB_2573

Alternate gene names: 86749691

Gene position: 2954839-2955453 (Clockwise)

Preceding gene: 86749690

Following gene: 86749692

Centisome position: 55.42

GC content: 65.85

Gene sequence:

>615_bases
TTGAGTCCAGCCTCGATGCAGCAGCCGACGCCGTCGACGCGCCCCGAGGGCGCGCAGCCGCTGATCGCGCGAGCCCCCAG
CGGCATCATCGATCCGAACACCGGCAAGCCGGTCGGCGCCGACGATCCGTTCTTTCTCGACGTCAATCGCGAGCTGTCCG
ACAAGGGATTCTTCGTCGCCGCGACCGACGATCTGATCACCTGGGCGCGCACCGGCTCGCTGATGTGGATGACCTTCGGC
CTGGCCTGCTGCGCGGTCGAGATGATGCAGTTGTCGATGCCGCGCTACGACGCCGAGCGGTTCGGCTTCGCCCCGCGCGC
TTCGCCGCGGCAGTCCGACGTGATGATCGTGGCCGGCACCCTGACCAATAAAATGGCTCCGGCGCTGCGTAAGGTCTACG
ATCAGATGCCGGAGCCGCGCTACGTGATCTCGATGGGATCGTGCGCCAACGGCGGCGGCTACTATCATTATTCCTACTCC
GTGGTGCGCGGCTGCGATCGCATCGTCCCGGTCGACATCTATGTGCCGGGGTGCCCGCCGACCGCGGAAGCTCTGCTGTA
CGGCGTGATGCTGCTGCAGAAGAAGATCCGTCGGACCGGCACGATCGAACGCTGA

Upstream 100 bases:

>100_bases
GGAAACTCGGCGCGACCGGCTTCTGGTCGATGATGGTGTTTCTCGGCGTGCTGACCGTCGGCTTCGCGTATGAGTGGAAG
AAGGGTGCGCTCGAATGGGA

Downstream 100 bases:

>100_bases
AGAAGAAGGCTGCTTCATGGACGACAACGGGCTCGACACCCTGGGTCAGACGATCGTCGGCGCCTTGCCGGGCGCCGCGA
TCGGTCATTCGATCGCATAC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2 [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSPASMQQPTPSTRPEGAQPLIARAPSGIIDPNTGKPVGADDPFFLDVNRELSDKGFFVAATDDLITWARTGSLMWMTFG
LACCAVEMMQLSMPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYS
VVRGCDRIVPVDIYVPGCPPTAEALLYGVMLLQKKIRRTGTIER

Sequences:

>Translated_204_residues
MSPASMQQPTPSTRPEGAQPLIARAPSGIIDPNTGKPVGADDPFFLDVNRELSDKGFFVAATDDLITWARTGSLMWMTFG
LACCAVEMMQLSMPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYS
VVRGCDRIVPVDIYVPGCPPTAEALLYGVMLLQKKIRRTGTIER
>Mature_203_residues
SPASMQQPTPSTRPEGAQPLIARAPSGIIDPNTGKPVGADDPFFLDVNRELSDKGFFVAATDDLITWARTGSLMWMTFGL
ACCAVEMMQLSMPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSV
VRGCDRIVPVDIYVPGCPPTAEALLYGVMLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=152, Percent_Identity=77.6315789473684, Blast_Score=250, Evalue=5e-67,
Organism=Escherichia coli, GI1788624, Length=175, Percent_Identity=46.2857142857143, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=44.4444444444444, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=42.2764227642276, Blast_Score=103, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=176, Percent_Identity=62.5, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI18859983, Length=155, Percent_Identity=72.258064516129, Blast_Score=247, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642371, Length=155, Percent_Identity=72.258064516129, Blast_Score=247, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24651058, Length=144, Percent_Identity=76.3888888888889, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 22368; Mature: 22237

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPASMQQPTPSTRPEGAQPLIARAPSGIIDPNTGKPVGADDPFFLDVNRELSDKGFFVA
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEE
ATDDLITWARTGSLMWMTFGLACCAVEMMQLSMPRYDAERFGFAPRASPRQSDVMIVAGT
ECCHHEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEEC
LTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPP
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCC
TAEALLYGVMLLQKKIRRTGTIER
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPASMQQPTPSTRPEGAQPLIARAPSGIIDPNTGKPVGADDPFFLDVNRELSDKGFFVA
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEE
ATDDLITWARTGSLMWMTFGLACCAVEMMQLSMPRYDAERFGFAPRASPRQSDVMIVAGT
ECCHHEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEEC
LTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPP
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCC
TAEALLYGVMLLQKKIRRTGTIER
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA