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The map label for this gene is nylA [H]

Identifier: 86749670

GI number: 86749670

Start: 2926326

End: 2927750

Strand: Direct

Name: nylA [H]

Synonym: RPB_2551

Alternate gene names: 86749670

Gene position: 2926326-2927750 (Clockwise)

Preceding gene: 86749669

Following gene: 86749671

Centisome position: 54.89

GC content: 69.26

Gene sequence:

>1425_bases
ATGGCATTCAAAGATTACTCCGACTACGACGGCGTCGGTCTCGCGGAGCTGGTGCGTCGCAAGCAGGTCACGCCCGGCGA
ACTGCTCGAGGAAGCGATCGCGCGGACGGACGCGCTCGATCCGAAGCTCAACGCCGTGGTGGTGCGTCATGACGACGACG
CGCGTCGGCAGATCGACAAGGGGTTGCCCGACGGGCCGTTCACGGGCGTGCCGTTCCTGCTGAAGGATCTCGATCTGCTC
GCCGGCACCCGCACGACGTTCGGCGCCAGCCTCTACAAGGATTTCGTCGCGCCGCACACCGGCACCCTGGCGGGCCGCTT
CCTGGCATCGGGCGTGACGATCTTCGGCAAGAGCGCCAGCCCCGAATTCGGGCTGATGCCGACCACCGAGTCGCGGCTGC
ACGGGCCGACGCGCAATCCGTGGAATCTCGATCATTCCTCCGGCGGATCGTCGGGCGGCGCCGCCGCGGCGGTGGCGGCG
CGGATTCTGCCGGTGGCGCATGCCAGCGACGGCGGCGGCTCGATCCGGATTCCCGCGTCGGCCTGCGGCGTGCTCGGGCT
GAAGCCGACCCGGGCGCGCAATCCGCTCGGACCGGACCGCGGCGAGGGCTGGGGCGGCTTCGCCTGCGGCCATGTGGTGA
GTATCAGCGTGCGCGACAGTGCGGCGATGCTGGATGCGCTGCACGGCCCCGAGCCGTCGAGCCCTTACGTCGCGCCGGCG
CCGGAGCGGCCGTTCGCGCAGGAGGTCGGCCGCGATCCCGGCCGGCTGCGGATCGCCTTCACCGATGCGTCGACTTATGG
CGATGCGATCGATCCCGAAATCGCCGCGGCGGTGCGCGACACCGCGAAGCTGCTCGAAGGCCTCGGCCATCACGTCGAGC
AGCGCGCGCCGAAGCTGCCGGCCGATCCCGCCGAGGTGATCGCCACCATCGTCGCCTGCAACACCGGGTTGACGGTGAAA
CTCGCCGAGCAGGCGATGGGCCGCGCCATGACTAATCGCGATTTCGAAACGCTGACGCTGGCCAGCGCCGCCAATGCTGG
CCGCGCCAGCGGGATCGACTATTGCGCCGCGCAGCTCGCCGCATTTCAGATCTCGCGTGCCGTCTCGGATTTCTTCACCG
ACATCGACGTGTTTTTGTCGCCGACGCTGTGCCTGCCGCCGCTGCGGATCGGCGAGATCGATACCATGGCGAGCGATTTG
TCCGGCATCAGCCCGCTGCTGCGGCGCTACATCCCCGGCACCAGCCTGTTCAACATGTCGGGCCAGCCGGCGATGTCGGT
GCCGCTGGCCGGGACCGCGAGCGGTCTGCCGATCGGCATGATGTTCGCGGGTCGGTTCGGCGACGAGGCGACGCTGTTGC
GTCTCGCTTCGCAGCTCGAGCAGGAGCGGCCCTGGAAAAACCGGCGACCGCCGGTTTGCGCCTGA

Upstream 100 bases:

>100_bases
AATCCAGCTCCGTGCACTGCGCCTCTGGATTGCTTCGCTTCGCTCGCAATGACGGGGCGAGGCGATAATGAAAGAACAAC
AATACGAAGGAGGCCGGCGA

Downstream 100 bases:

>100_bases
TTGAGTCCGACTTTTCGCCGGTATTCTCGGAAACAAACGACCAAGGTCGGCGTTGACGCGCGGCCTCGGCCGTCATTTCG
TATCGTATCTCAGAGGGACC

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAFKDYSDYDGVGLAELVRRKQVTPGELLEEAIARTDALDPKLNAVVVRHDDDARRQIDKGLPDGPFTGVPFLLKDLDLL
AGTRTTFGASLYKDFVAPHTGTLAGRFLASGVTIFGKSASPEFGLMPTTESRLHGPTRNPWNLDHSSGGSSGGAAAAVAA
RILPVAHASDGGGSIRIPASACGVLGLKPTRARNPLGPDRGEGWGGFACGHVVSISVRDSAAMLDALHGPEPSSPYVAPA
PERPFAQEVGRDPGRLRIAFTDASTYGDAIDPEIAAAVRDTAKLLEGLGHHVEQRAPKLPADPAEVIATIVACNTGLTVK
LAEQAMGRAMTNRDFETLTLASAANAGRASGIDYCAAQLAAFQISRAVSDFFTDIDVFLSPTLCLPPLRIGEIDTMASDL
SGISPLLRRYIPGTSLFNMSGQPAMSVPLAGTASGLPIGMMFAGRFGDEATLLRLASQLEQERPWKNRRPPVCA

Sequences:

>Translated_474_residues
MAFKDYSDYDGVGLAELVRRKQVTPGELLEEAIARTDALDPKLNAVVVRHDDDARRQIDKGLPDGPFTGVPFLLKDLDLL
AGTRTTFGASLYKDFVAPHTGTLAGRFLASGVTIFGKSASPEFGLMPTTESRLHGPTRNPWNLDHSSGGSSGGAAAAVAA
RILPVAHASDGGGSIRIPASACGVLGLKPTRARNPLGPDRGEGWGGFACGHVVSISVRDSAAMLDALHGPEPSSPYVAPA
PERPFAQEVGRDPGRLRIAFTDASTYGDAIDPEIAAAVRDTAKLLEGLGHHVEQRAPKLPADPAEVIATIVACNTGLTVK
LAEQAMGRAMTNRDFETLTLASAANAGRASGIDYCAAQLAAFQISRAVSDFFTDIDVFLSPTLCLPPLRIGEIDTMASDL
SGISPLLRRYIPGTSLFNMSGQPAMSVPLAGTASGLPIGMMFAGRFGDEATLLRLASQLEQERPWKNRRPPVCA
>Mature_473_residues
AFKDYSDYDGVGLAELVRRKQVTPGELLEEAIARTDALDPKLNAVVVRHDDDARRQIDKGLPDGPFTGVPFLLKDLDLLA
GTRTTFGASLYKDFVAPHTGTLAGRFLASGVTIFGKSASPEFGLMPTTESRLHGPTRNPWNLDHSSGGSSGGAAAAVAAR
ILPVAHASDGGGSIRIPASACGVLGLKPTRARNPLGPDRGEGWGGFACGHVVSISVRDSAAMLDALHGPEPSSPYVAPAP
ERPFAQEVGRDPGRLRIAFTDASTYGDAIDPEIAAAVRDTAKLLEGLGHHVEQRAPKLPADPAEVIATIVACNTGLTVKL
AEQAMGRAMTNRDFETLTLASAANAGRASGIDYCAAQLAAFQISRAVSDFFTDIDVFLSPTLCLPPLRIGEIDTMASDLS
GISPLLRRYIPGTSLFNMSGQPAMSVPLAGTASGLPIGMMFAGRFGDEATLLRLASQLEQERPWKNRRPPVCA

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=24.3083003952569, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI195972892, Length=240, Percent_Identity=27.5, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI166795287, Length=292, Percent_Identity=27.7397260273973, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=197, Percent_Identity=30.4568527918782, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=27.6666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=159, Percent_Identity=35.2201257861635, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=25.0497017892644, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=28.4848484848485, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356731, Length=298, Percent_Identity=30.2013422818792, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550774, Length=193, Percent_Identity=31.0880829015544, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648435, Length=193, Percent_Identity=31.0880829015544, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648437, Length=193, Percent_Identity=31.0880829015544, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=24.65483234714, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652985, Length=192, Percent_Identity=28.6458333333333, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922090, Length=192, Percent_Identity=28.6458333333333, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652981, Length=192, Percent_Identity=28.6458333333333, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652983, Length=192, Percent_Identity=28.6458333333333, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 49873; Mature: 49742

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKDYSDYDGVGLAELVRRKQVTPGELLEEAIARTDALDPKLNAVVVRHDDDARRQIDK
CCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHC
GLPDGPFTGVPFLLKDLDLLAGTRTTFGASLYKDFVAPHTGTLAGRFLASGVTIFGKSAS
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCC
PEFGLMPTTESRLHGPTRNPWNLDHSSGGSSGGAAAAVAARILPVAHASDGGGSIRIPAS
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEECHH
ACGVLGLKPTRARNPLGPDRGEGWGGFACGHVVSISVRDSAAMLDALHGPEPSSPYVAPA
HCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCHHHHHHHHCCCCCCCCCCCCC
PERPFAQEVGRDPGRLRIAFTDASTYGDAIDPEIAAAVRDTAKLLEGLGHHVEQRAPKLP
CCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADPAEVIATIVACNTGLTVKLAEQAMGRAMTNRDFETLTLASAANAGRASGIDYCAAQLA
CCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCHHHHHHHHH
AFQISRAVSDFFTDIDVFLSPTLCLPPLRIGEIDTMASDLSGISPLLRRYIPGTSLFNMS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCC
GQPAMSVPLAGTASGLPIGMMFAGRFGDEATLLRLASQLEQERPWKNRRPPVCA
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AFKDYSDYDGVGLAELVRRKQVTPGELLEEAIARTDALDPKLNAVVVRHDDDARRQIDK
CCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHC
GLPDGPFTGVPFLLKDLDLLAGTRTTFGASLYKDFVAPHTGTLAGRFLASGVTIFGKSAS
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCC
PEFGLMPTTESRLHGPTRNPWNLDHSSGGSSGGAAAAVAARILPVAHASDGGGSIRIPAS
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEECHH
ACGVLGLKPTRARNPLGPDRGEGWGGFACGHVVSISVRDSAAMLDALHGPEPSSPYVAPA
HCCCCCCCCCCCCCCCCCCCCCCCCCEECCEEEEEEECCHHHHHHHHCCCCCCCCCCCCC
PERPFAQEVGRDPGRLRIAFTDASTYGDAIDPEIAAAVRDTAKLLEGLGHHVEQRAPKLP
CCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADPAEVIATIVACNTGLTVKLAEQAMGRAMTNRDFETLTLASAANAGRASGIDYCAAQLA
CCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCHHHHHHHHH
AFQISRAVSDFFTDIDVFLSPTLCLPPLRIGEIDTMASDLSGISPLLRRYIPGTSLFNMS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCC
GQPAMSVPLAGTASGLPIGMMFAGRFGDEATLLRLASQLEQERPWKNRRPPVCA
CCCCEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]