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The map label for this gene is ksgA

Identifier: 86749595

GI number: 86749595

Start: 2831435

End: 2832298

Strand: Reverse

Name: ksgA

Synonym: RPB_2475

Alternate gene names: 86749595

Gene position: 2832298-2831435 (Counterclockwise)

Preceding gene: 86749596

Following gene: 86749594

Centisome position: 53.12

GC content: 68.98

Gene sequence:

>864_bases
ATGAGTGCGATCGACGGCCTGCCGCCGCTGCGCGACGTCATCAAGCGTCACGATCTCGCCGCGCGAAAATCGCTCGGCCA
GAATTTTCTGCTCGACCTCAATCTCACCGCCCGGATCGCGCGCGCCGCCGGCCCGCTGGACGACGTGACCGTGGTCGAGA
TCGGCCCCGGCCCCGGCGGATTGACCCGCGCGCTGCTCGCCACCGGCGCGAGGCGCGTGATCGCGATCGAACGCGACGAA
CGCGCCCTCGGCGCACTCGAGGAAATCGCGGCGCACTATCCGGGACGGCTGGAGATCGTCTGCGCCGACGCCATGGAGTT
CGACCCCCGGCCTTTGCTCGGCGGAGCACGCGCCAGGATCGTCGCCAACCTGCCCTACAACATCGCCACGCCGCTCCTGA
TCGGCTGGCTCTGCGCCGAGCCGTGGCCCCCCTGGTACGACATGATGGTGCTGATGTTTCAGCGCGAAGTGGCGCAACGC
ATCGTCGCCTGCGAGGACGACGACGCCTATGGCCGGCTCGCGGTGCTGTCGAACTGGCGGTGCGACACCGGGATGCTGTT
CGACATCGCGCCCTCCGCCTTCGTGCCGCAGCCGAAGGTGACCTCCTCCGTCGTCCGCCTGGTCCCGCGCTCCGCGCCCG
AACCATGCAACCGCGCGGCGCTGGAGCAGGTCGCCGCCGCCGCCTTCGGCCAGCGCCGCAAGATGCTGCGGCAAAGCCTC
AAGGCGCTCGGCGTCGACCCGGCGCGGCTGGCTGCGGCGGCCGGGATCGACCCGACACGCCGCGCCGAGACCGTCCCGGT
TTCCGGCTTTGTTGCCATGGCGAACGAATTGATCGATATACGAGATACAAAAAACACACCCTAA

Upstream 100 bases:

>100_bases
ACGGCACGGCATTCGACATCGCCGGCACCGGACGCGCCGATCCGTCGAGCCTGATCGCCGCGCTGAAGCTCGCGGCCCGG
ATGGCTGCGGCGCCTTCGCC

Downstream 100 bases:

>100_bases
GGGAGAGACCGACATGACGCAGATGCGTCGCCAATCGCTGGTGAAATTCGACGCCCCGCTGTGCGAGACCATTATCGACA
CGCCGAAGCCGCAAGGCCGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSAIDGLPPLRDVIKRHDLAARKSLGQNFLLDLNLTARIARAAGPLDDVTVVEIGPGPGGLTRALLATGARRVIAIERDE
RALGALEEIAAHYPGRLEIVCADAMEFDPRPLLGGARARIVANLPYNIATPLLIGWLCAEPWPPWYDMMVLMFQREVAQR
IVACEDDDAYGRLAVLSNWRCDTGMLFDIAPSAFVPQPKVTSSVVRLVPRSAPEPCNRAALEQVAAAAFGQRRKMLRQSL
KALGVDPARLAAAAGIDPTRRAETVPVSGFVAMANELIDIRDTKNTP

Sequences:

>Translated_287_residues
MSAIDGLPPLRDVIKRHDLAARKSLGQNFLLDLNLTARIARAAGPLDDVTVVEIGPGPGGLTRALLATGARRVIAIERDE
RALGALEEIAAHYPGRLEIVCADAMEFDPRPLLGGARARIVANLPYNIATPLLIGWLCAEPWPPWYDMMVLMFQREVAQR
IVACEDDDAYGRLAVLSNWRCDTGMLFDIAPSAFVPQPKVTSSVVRLVPRSAPEPCNRAALEQVAAAAFGQRRKMLRQSL
KALGVDPARLAAAAGIDPTRRAETVPVSGFVAMANELIDIRDTKNTP
>Mature_286_residues
SAIDGLPPLRDVIKRHDLAARKSLGQNFLLDLNLTARIARAAGPLDDVTVVEIGPGPGGLTRALLATGARRVIAIERDER
ALGALEEIAAHYPGRLEIVCADAMEFDPRPLLGGARARIVANLPYNIATPLLIGWLCAEPWPPWYDMMVLMFQREVAQRI
VACEDDDAYGRLAVLSNWRCDTGMLFDIAPSAFVPQPKVTSSVVRLVPRSAPEPCNRAALEQVAAAAFGQRRKMLRQSLK
ALGVDPARLAAAAGIDPTRRAETVPVSGFVAMANELIDIRDTKNTP

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=291, Percent_Identity=35.0515463917526, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=32.4675324675325, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786236, Length=263, Percent_Identity=38.4030418250951, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=33.8926174496644, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=280, Percent_Identity=27.8571428571429, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21357273, Length=252, Percent_Identity=35.7142857142857, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21358017, Length=235, Percent_Identity=33.1914893617021, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_RHOP2 (Q2IX80)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486091.1
- ProteinModelPortal:   Q2IX80
- STRING:   Q2IX80
- GeneID:   3910264
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2475
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   RPAL316058:RPB_2475-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31086; Mature: 30955

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDGLPPLRDVIKRHDLAARKSLGQNFLLDLNLTARIARAAGPLDDVTVVEIGPGPGG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCCCH
LTRALLATGARRVIAIERDERALGALEEIAAHYPGRLEIVCADAMEFDPRPLLGGARARI
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEE
VANLPYNIATPLLIGWLCAEPWPPWYDMMVLMFQREVAQRIVACEDDDAYGRLAVLSNWR
EEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
CDTGMLFDIAPSAFVPQPKVTSSVVRLVPRSAPEPCNRAALEQVAAAAFGQRRKMLRQSL
CCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KALGVDPARLAAAAGIDPTRRAETVPVSGFVAMANELIDIRDTKNTP
HHHCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAIDGLPPLRDVIKRHDLAARKSLGQNFLLDLNLTARIARAAGPLDDVTVVEIGPGPGG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCCCH
LTRALLATGARRVIAIERDERALGALEEIAAHYPGRLEIVCADAMEFDPRPLLGGARARI
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEE
VANLPYNIATPLLIGWLCAEPWPPWYDMMVLMFQREVAQRIVACEDDDAYGRLAVLSNWR
EEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
CDTGMLFDIAPSAFVPQPKVTSSVVRLVPRSAPEPCNRAALEQVAAAAFGQRRKMLRQSL
CCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KALGVDPARLAAAAGIDPTRRAETVPVSGFVAMANELIDIRDTKNTP
HHHCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA