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The map label for this gene is purF [H]

Identifier: 86749572

GI number: 86749572

Start: 2808256

End: 2809800

Strand: Reverse

Name: purF [H]

Synonym: RPB_2452

Alternate gene names: 86749572

Gene position: 2809800-2808256 (Counterclockwise)

Preceding gene: 86749573

Following gene: 86749571

Centisome position: 52.7

GC content: 66.41

Gene sequence:

>1545_bases
ATGGACGCGATCAACAGCTCTTCCGACGACGCCGCAACGCCCGCTCTGGACGCCCGCGACACGCAAGATCTCGATAACCA
TATTCTGGATAACGACGTGTTCGATACCGACGGCGATACGCTGCGGGAAGAATGCGGCGTCTTCGGCATCTTCGGCCATC
CCGACGCCGCCGCCATCACCGCGCTCGGTCTGCACGCCCTGCAGCACCGCGGACAGGAAGCCGCCGGCATCGTCTCCTTC
GACAACGGTCGCTTCCACTCGGAGCGCCGGCTCGGTCTGGTCGGCGACACCTTCTCCCGCGCCGACGTGATCGCCCGGCT
GCCCGGCAATCTTGCGGTCGGCCACGTCCGCTATTCCACCACCGGCGAGACCATCCTGCGCAACGTGCAGCCGCTGTTCG
CAGAGCTGAACGCCGGCGGCTTCGCGGTCGGCCACAACGGCAATCTCACCAACGGCCTGACGCTGCGCCGCGAACTGGTG
CAGAGCGGCGCGATCATGCAGTCGACCACCGACACCGAGGTGATCCTGCATCTGGTCGCGCAATCCAAGCGTGGCCGCTT
CATCGACCGCTTCATCGAGGCGTTGCGCGCGATCGAGGGCGCCTACTCGCTGGTGGCCCTGACCAATAAGAAGCTGATCG
GCGCCCGCGATCCGCTCGGCATCCGCCCGCTGGTGCTGGGCGAGCTCGACGGCTGTCCGATCCTGGCATCCGAGACCTGC
GCGCTCGACATCATCGGCGCGAAATACGTCCGCGACGTCGAGCCCGGCGAAATCATCGTATTCGACGAGAGCGGGGCGCA
GAGCCACAAGCCGTTCCCGCCGCAGCCGCCGCGACCCTGCATCTTCGAGTACATCTACTTCGCGCGGCCGGATTCGATCG
TCGGCGGTCGCTCGGTCTACGACGTCCGCAAGGGGTTCGGCGCCGAACTGGCGCGCGAAAGCCACGTTGCAGCCGACGTG
GTGGTGCCGGTGCCGGATTCCGGCGTGCCGGCGGCGATCGGCTACAGCCGCGAAAGCGGCGTGCCGTTCGAGCTCGGCAT
CATCCGCAATCATTATGTCGGGCGCACTTTCATTCAGCCGACCCAGAGCGTCCGCGAACTCGGCGTGCGGATGAAGCATT
CCGCCAATCGCGCGGCGATCGAAGGCAAGCGGATCGTCCTGATCGACGACAGCCTGGTGCGCGGCACCACCTCGAAGAAG
ATCGTCAAGATGATGCGCGACGCCGGCGCCAAGGAAGTTCACTTCCGCATCGCCTCGCCGCCGATCACGCATCCGGACTA
TTACGGCATCGACACCCCGGACCGCGCCGGCCTGCTCGCGGCGACGCGCTCGCTCGAAGAGATGCGAGATCTGATCGGTG
CGGATTCGCTGGCCTTCCTGTCGGTCGACGGCATCTATCGCGCGATGGGTGAACCGCGCCGCGACCCGGCGCATCCGACC
TACACCGACCATTGCTTCACCGGCGACTATCCGACGCCGCTGACGGACCTGAACCAGACCGAGGCGTCGCCGCGTCAGTT
GTCGCTGCTGGCCGAAGCCAGCTGA

Upstream 100 bases:

>100_bases
GGCAGTGGCGACGGCTACAGCAAACCCGCCCGCGACAGTTTGAAAAAGCTGATAGAGGGCAAACCCGACGCACGCTGACG
CGTGCTCAGTGAGAGGCGCG

Downstream 100 bases:

>100_bases
CGGCTCACCCCGTCCTTCTTACTAAAGTTCGTCGTGCCCGGCCTTGTGCCGGGCATCCACGTCTTTAGAAGGTCGTGGAT
GACCGGGGCCAGCCCGGCCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDAINSSSDDAATPALDARDTQDLDNHILDNDVFDTDGDTLREECGVFGIFGHPDAAAITALGLHALQHRGQEAAGIVSF
DNGRFHSERRLGLVGDTFSRADVIARLPGNLAVGHVRYSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELV
QSGAIMQSTTDTEVILHLVAQSKRGRFIDRFIEALRAIEGAYSLVALTNKKLIGARDPLGIRPLVLGELDGCPILASETC
ALDIIGAKYVRDVEPGEIIVFDESGAQSHKPFPPQPPRPCIFEYIYFARPDSIVGGRSVYDVRKGFGAELARESHVAADV
VVPVPDSGVPAAIGYSRESGVPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKRIVLIDDSLVRGTTSKK
IVKMMRDAGAKEVHFRIASPPITHPDYYGIDTPDRAGLLAATRSLEEMRDLIGADSLAFLSVDGIYRAMGEPRRDPAHPT
YTDHCFTGDYPTPLTDLNQTEASPRQLSLLAEAS

Sequences:

>Translated_514_residues
MDAINSSSDDAATPALDARDTQDLDNHILDNDVFDTDGDTLREECGVFGIFGHPDAAAITALGLHALQHRGQEAAGIVSF
DNGRFHSERRLGLVGDTFSRADVIARLPGNLAVGHVRYSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELV
QSGAIMQSTTDTEVILHLVAQSKRGRFIDRFIEALRAIEGAYSLVALTNKKLIGARDPLGIRPLVLGELDGCPILASETC
ALDIIGAKYVRDVEPGEIIVFDESGAQSHKPFPPQPPRPCIFEYIYFARPDSIVGGRSVYDVRKGFGAELARESHVAADV
VVPVPDSGVPAAIGYSRESGVPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKRIVLIDDSLVRGTTSKK
IVKMMRDAGAKEVHFRIASPPITHPDYYGIDTPDRAGLLAATRSLEEMRDLIGADSLAFLSVDGIYRAMGEPRRDPAHPT
YTDHCFTGDYPTPLTDLNQTEASPRQLSLLAEAS
>Mature_514_residues
MDAINSSSDDAATPALDARDTQDLDNHILDNDVFDTDGDTLREECGVFGIFGHPDAAAITALGLHALQHRGQEAAGIVSF
DNGRFHSERRLGLVGDTFSRADVIARLPGNLAVGHVRYSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELV
QSGAIMQSTTDTEVILHLVAQSKRGRFIDRFIEALRAIEGAYSLVALTNKKLIGARDPLGIRPLVLGELDGCPILASETC
ALDIIGAKYVRDVEPGEIIVFDESGAQSHKPFPPQPPRPCIFEYIYFARPDSIVGGRSVYDVRKGFGAELARESHVAADV
VVPVPDSGVPAAIGYSRESGVPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKRIVLIDDSLVRGTTSKK
IVKMMRDAGAKEVHFRIASPPITHPDYYGIDTPDRAGLLAATRSLEEMRDLIGADSLAFLSVDGIYRAMGEPRRDPAHPT
YTDHCFTGDYPTPLTDLNQTEASPRQLSLLAEAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=465, Percent_Identity=38.9247311827957, Blast_Score=329, Evalue=4e-90,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=39.7058823529412, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1790167, Length=237, Percent_Identity=32.0675105485232, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=39.0396659707724, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6323958, Length=499, Percent_Identity=33.0661322645291, Blast_Score=253, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=41.2121212121212, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=41.7558886509636, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=41.4141414141414, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55694; Mature: 55694

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAINSSSDDAATPALDARDTQDLDNHILDNDVFDTDGDTLREECGVFGIFGHPDAAAIT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCHHHHH
ALGLHALQHRGQEAAGIVSFDNGRFHSERRLGLVGDTFSRADVIARLPGNLAVGHVRYST
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEEEECC
TGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVQSGAIMQSTTDTEVILHLVA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHH
QSKRGRFIDRFIEALRAIEGAYSLVALTNKKLIGARDPLGIRPLVLGELDGCPILASETC
HCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCCCCCEEEECCCCCCEECCCCE
ALDIIGAKYVRDVEPGEIIVFDESGAQSHKPFPPQPPRPCIFEYIYFARPDSIVGGRSVY
EEEEECHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHH
DVRKGFGAELARESHVAADVVVPVPDSGVPAAIGYSRESGVPFELGIIRNHYVGRTFIQP
HHHHCCCHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHCCH
TQSVRELGVRMKHSANRAAIEGKRIVLIDDSLVRGTTSKKIVKMMRDAGAKEVHFRIASP
HHHHHHHHHHHHCCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
PITHPDYYGIDTPDRAGLLAATRSLEEMRDLIGADSLAFLSVDGIYRAMGEPRRDPAHPT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCC
YTDHCFTGDYPTPLTDLNQTEASPRQLSLLAEAS
CCCCEECCCCCCCCCCCCCCCCCCHHEEEEECCC
>Mature Secondary Structure
MDAINSSSDDAATPALDARDTQDLDNHILDNDVFDTDGDTLREECGVFGIFGHPDAAAIT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCEEEEECCCCHHHHH
ALGLHALQHRGQEAAGIVSFDNGRFHSERRLGLVGDTFSRADVIARLPGNLAVGHVRYST
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEEEECC
TGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVQSGAIMQSTTDTEVILHLVA
CHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHH
QSKRGRFIDRFIEALRAIEGAYSLVALTNKKLIGARDPLGIRPLVLGELDGCPILASETC
HCCCCHHHHHHHHHHHHHHHHHEEEEEECCCEECCCCCCCCCCEEEECCCCCCEECCCCE
ALDIIGAKYVRDVEPGEIIVFDESGAQSHKPFPPQPPRPCIFEYIYFARPDSIVGGRSVY
EEEEECHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHH
DVRKGFGAELARESHVAADVVVPVPDSGVPAAIGYSRESGVPFELGIIRNHYVGRTFIQP
HHHHCCCHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHCCH
TQSVRELGVRMKHSANRAAIEGKRIVLIDDSLVRGTTSKKIVKMMRDAGAKEVHFRIASP
HHHHHHHHHHHHCCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCC
PITHPDYYGIDTPDRAGLLAATRSLEEMRDLIGADSLAFLSVDGIYRAMGEPRRDPAHPT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCC
YTDHCFTGDYPTPLTDLNQTEASPRQLSLLAEAS
CCCCEECCCCCCCCCCCCCCCCCCHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA