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The map label for this gene is panB

Identifier: 86749565

GI number: 86749565

Start: 2801019

End: 2801843

Strand: Direct

Name: panB

Synonym: RPB_2445

Alternate gene names: 86749565

Gene position: 2801019-2801843 (Clockwise)

Preceding gene: 86749564

Following gene: 86749566

Centisome position: 52.54

GC content: 66.79

Gene sequence:

>825_bases
ATGTCCGTCCAAACGACCATCAAGCGGAAGACCGCTCCGGACATCCGCGCGCGCAAGGGCGGCGATCCGATCGTGATGCT
GACCTCGTATCACGCCCACACCGCCTCGCTGGTCGATCGCTATTGCGACGTCATCCTGGTCGGAGACTCGCTCGGCAATG
TGATGCACGGCTTCGAGACCACCATCCCGGTGACGCTCGAGATGATGATCCTGCAGGGCCACGCGGTGATGCGCGGCTCG
CAGCACGCGCTGGTCGTCGTCGACATGCCTTTCGGCTCCTACGAAGCGTCGAAGGAGCAGGCGTTTCACTCCGCGGCGCG
CATCCTGAAAGAGACGCATTGCGGCGCGGTGAAGCTCGAGGGTGGCGTGCGGATGGCGGAGACCATCGCGTTTCTCACCG
AGCGCGGCATTCCGGTGATGGGCCATATCGGGTTGACGCCGCAATCGATCAACACGCTGGGCTCGTTCCGCGCGCAGGGG
CGCGAGGAGGGCAGCTGGGAGCCGATCGAGGCGGACGCGCGCGCCGTCGCCGATGCGGGCGCCTTCTCCGTCGTGGTCGA
GGCGGTGGCCGAGCCGCTCGGGCGCAAGATCACCGAGACGATCGCGATCCCGACCATCGGCATCGGCGCCAGCGCCGCCT
GTGACGGTCAGGTACTGGTGCTCGAGGACATGCTCGGGCTGTCGCCGCGCACCCCGAAATTCGTCAAGCGCTACGGTGAA
CTCGGGCCGGGCATCGAAGCGGCGATCAAGGGCTATGCCGAAGAGGTGCGGTCGCGCGCCTTCCCGGGGCCGGAACACGT
CTACGGAATGAAGGCGAAGGGCTGA

Upstream 100 bases:

>100_bases
ACGACGCCATCGCGATCGGCGCCGGCGTGGTGCTGTATCTGGCGCTGGCGTTCGCCTTCCATCCCTACGTCGTCGGCGTT
CCCGTCTTCGGAGCCTAAGC

Downstream 100 bases:

>100_bases
GGCTGGCGCGCCGGTTAATCATATCGGGGCATCCCCTTGATCCGGCTGGCTTTGCCTTGCCTCCGCCATAACGCTAGGGT
ATCGCCGCCGCGACGTCGGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSVQTTIKRKTAPDIRARKGGDPIVMLTSYHAHTASLVDRYCDVILVGDSLGNVMHGFETTIPVTLEMMILQGHAVMRGS
QHALVVVDMPFGSYEASKEQAFHSAARILKETHCGAVKLEGGVRMAETIAFLTERGIPVMGHIGLTPQSINTLGSFRAQG
REEGSWEPIEADARAVADAGAFSVVVEAVAEPLGRKITETIAIPTIGIGASAACDGQVLVLEDMLGLSPRTPKFVKRYGE
LGPGIEAAIKGYAEEVRSRAFPGPEHVYGMKAKG

Sequences:

>Translated_274_residues
MSVQTTIKRKTAPDIRARKGGDPIVMLTSYHAHTASLVDRYCDVILVGDSLGNVMHGFETTIPVTLEMMILQGHAVMRGS
QHALVVVDMPFGSYEASKEQAFHSAARILKETHCGAVKLEGGVRMAETIAFLTERGIPVMGHIGLTPQSINTLGSFRAQG
REEGSWEPIEADARAVADAGAFSVVVEAVAEPLGRKITETIAIPTIGIGASAACDGQVLVLEDMLGLSPRTPKFVKRYGE
LGPGIEAAIKGYAEEVRSRAFPGPEHVYGMKAKG
>Mature_273_residues
SVQTTIKRKTAPDIRARKGGDPIVMLTSYHAHTASLVDRYCDVILVGDSLGNVMHGFETTIPVTLEMMILQGHAVMRGSQ
HALVVVDMPFGSYEASKEQAFHSAARILKETHCGAVKLEGGVRMAETIAFLTERGIPVMGHIGLTPQSINTLGSFRAQGR
EEGSWEPIEADARAVADAGAFSVVVEAVAEPLGRKITETIAIPTIGIGASAACDGQVLVLEDMLGLSPRTPKFVKRYGEL
GPGIEAAIKGYAEEVRSRAFPGPEHVYGMKAKG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=244, Percent_Identity=42.2131147540984, Blast_Score=186, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319653, Length=271, Percent_Identity=35.0553505535055, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RHOP2 (Q2IXB0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486061.1
- ProteinModelPortal:   Q2IXB0
- SMR:   Q2IXB0
- STRING:   Q2IXB0
- GeneID:   3910234
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2445
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   RPAL316058:RPB_2445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29238; Mature: 29107

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 88-88 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQTTIKRKTAPDIRARKGGDPIVMLTSYHAHTASLVDRYCDVILVGDSLGNVMHGFET
CCCCCHHHHCCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCHHHHHHHCCCC
TIPVTLEMMILQGHAVMRGSQHALVVVDMPFGSYEASKEQAFHSAARILKETHCGAVKLE
CCCCEEEEHHHHCCHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GGVRMAETIAFLTERGIPVMGHIGLTPQSINTLGSFRAQGREEGSWEPIEADARAVADAG
CCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCH
AFSVVVEAVAEPLGRKITETIAIPTIGIGASAACDGQVLVLEDMLGLSPRTPKFVKRYGE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHC
LGPGIEAAIKGYAEEVRSRAFPGPEHVYGMKAKG
CCCCHHHHHHHHHHHHHHHCCCCHHHEECCCCCC
>Mature Secondary Structure 
SVQTTIKRKTAPDIRARKGGDPIVMLTSYHAHTASLVDRYCDVILVGDSLGNVMHGFET
CCCCHHHHCCCCCCHHCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCHHHHHHHCCCC
TIPVTLEMMILQGHAVMRGSQHALVVVDMPFGSYEASKEQAFHSAARILKETHCGAVKLE
CCCCEEEEHHHHCCHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
GGVRMAETIAFLTERGIPVMGHIGLTPQSINTLGSFRAQGREEGSWEPIEADARAVADAG
CCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCH
AFSVVVEAVAEPLGRKITETIAIPTIGIGASAACDGQVLVLEDMLGLSPRTPKFVKRYGE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHC
LGPGIEAAIKGYAEEVRSRAFPGPEHVYGMKAKG
CCCCHHHHHHHHHHHHHHHCCCCHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA