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The map label for this gene is gatA

Identifier: 86749558

GI number: 86749558

Start: 2793329

End: 2794807

Strand: Direct

Name: gatA

Synonym: RPB_2438

Alternate gene names: 86749558

Gene position: 2793329-2794807 (Clockwise)

Preceding gene: 86749555

Following gene: 86749559

Centisome position: 52.39

GC content: 67.21

Gene sequence:

>1479_bases
ATGACCGATCTGACTTCGCTGACGCTGGCCGAGGCCCGCGATGGCCTCGTGAACAAGTCCTTCACCGCGGTCGAACTGAC
CGACGCGCATCTCGCCGCAATCGAAGCCGCGCGTGTGCTCAACGCCTATGTGCTGGAGACGCCGGATCAGGCACGGCAGA
TGGCGCAGGCGGCGGATGCGCAGATCGCCAAAGGCGAGGGCGGGCCGCTCGCCGGCATCCCGCTCGGCATCAAGGATCTG
TTCGCCACCAAAGGCACGCGCACCACCGCCTGCTCGAAAATTCTCGGAGACTTCAAGCCTCCCTACGAGTCGACCGTGAC
GTCGCAGCTGTGGCGCGACGGCGCCGTGCTGCTCGGCAAGCTCAACAACGACGAATTCGCGATGGGCTCGTCGAACGAGA
CATCGTGCTTCGGCCCGGTGGTCAACCCATGGCGGCGCGCGGGCTCGGAGACCAAGCTGGTTCCTGGCGGCTCGTCCGGC
GGCTCGGCGGCGGCGGTGGCCGCCGGCCTCTGCCTCGGCGCCACCGCCACCGACACCGGCGGGTCGATCCGGCAGCCGGC
GGCGTTCACCGGCACCGTCGGCATCAAGCCGACCTATGGCCGCTGCTCGCGCTGGGGCATCGTCGCGTTTGCGTCCTCGC
TCGACCAGGCCGGTCCGATTGCCCGCACGGTGCGCGACAGCGCGATCCTGCTCGGCTCGATGGCCGGCTTTGACCCGAAG
GACACAACCTCGGTGGATCGCCCAGTGCCGAACTACGAAGCCGCGATCGGCGGCTCGGTGAAGGGCATGAAGATCGGCAT
CCCGAAGGAGTATCGGCTCGACGGCATGCCGGCCGAGATCGAGAAATTGTGGATGCAGGGCGCCGAGTGGCTGAAAGCGG
CGGGCGCCGAATTGGTCGAGGTGTCGCTGCCGCACACCAAATACGCACTGCCGGCCTATTACATCGTGGCGCCGGCGGAA
GCCTCGTCGAACCTCGCGCGCTACGACGGCGTGCGCTACGGCACCCGCGTCAACGGCCGCAACATCGTCGAGATGTACGA
GAACACCCGCGCGGCCGGTTTCGGCGCCGAGGTCAAGCGTCGCATCATGATCGGAACCTATGTGCTGTCGGCGGGCTATT
ACGACGCCTATTATCTCCGCGCCCAGAAGGTCCGCACCCTGATCAAGCGCGATTTCGAGCAGTGCTTCGACCAGGGCGTC
TCCGCGATCCTGACGCCCGCGACGCCATCGGCGGCGTTCGGAATCGGCGAAAAAGGCGGCGCCGATCCGGTCGAGATGTA
CCTGAACGACATCTTCACCGTGACGGTGAACATGGCGGGCCTGCCGGGCATCGCGGTTCCGGCGGGGAGCGACTCGCAGG
GACTTCCGCTGGGGCTGCAACTGATCGGGCGGCCGTTCGACGAGGAGACGTTGTTCTCCCTCGGCGACGTCATCGAGCAG
TCCGCCGGCCGGTTCACGCCGGCTAAGTGGTGGGCGTAG

Upstream 100 bases:

>100_bases
GTCGAAGGCGCTGTGCTAGCCAGCGCTGCAACAAAGCCGCACGGATCCGCGGCGCCCGTGACCGGCCCGGCGCCGGCTTC
AATCCAGGCGTGCCTGAACC

Downstream 100 bases:

>100_bases
CCTGCGCGCGATGCACCGGCTTTTGCGTTGCGTTTCCCGTTTTGTTTCGCCATTCTCGGATGGGTGGGTAGGATTTGTTG
AATGAGCGCCGATGAAGGGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTDLTSLTLAEARDGLVNKSFTAVELTDAHLAAIEAARVLNAYVLETPDQARQMAQAADAQIAKGEGGPLAGIPLGIKDL
FATKGTRTTACSKILGDFKPPYESTVTSQLWRDGAVLLGKLNNDEFAMGSSNETSCFGPVVNPWRRAGSETKLVPGGSSG
GSAAAVAAGLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDSAILLGSMAGFDPK
DTTSVDRPVPNYEAAIGGSVKGMKIGIPKEYRLDGMPAEIEKLWMQGAEWLKAAGAELVEVSLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGTRVNGRNIVEMYENTRAAGFGAEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFEQCFDQGV
SAILTPATPSAAFGIGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGSDSQGLPLGLQLIGRPFDEETLFSLGDVIEQ
SAGRFTPAKWWA

Sequences:

>Translated_492_residues
MTDLTSLTLAEARDGLVNKSFTAVELTDAHLAAIEAARVLNAYVLETPDQARQMAQAADAQIAKGEGGPLAGIPLGIKDL
FATKGTRTTACSKILGDFKPPYESTVTSQLWRDGAVLLGKLNNDEFAMGSSNETSCFGPVVNPWRRAGSETKLVPGGSSG
GSAAAVAAGLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDSAILLGSMAGFDPK
DTTSVDRPVPNYEAAIGGSVKGMKIGIPKEYRLDGMPAEIEKLWMQGAEWLKAAGAELVEVSLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGTRVNGRNIVEMYENTRAAGFGAEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFEQCFDQGV
SAILTPATPSAAFGIGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGSDSQGLPLGLQLIGRPFDEETLFSLGDVIEQ
SAGRFTPAKWWA
>Mature_491_residues
TDLTSLTLAEARDGLVNKSFTAVELTDAHLAAIEAARVLNAYVLETPDQARQMAQAADAQIAKGEGGPLAGIPLGIKDLF
ATKGTRTTACSKILGDFKPPYESTVTSQLWRDGAVLLGKLNNDEFAMGSSNETSCFGPVVNPWRRAGSETKLVPGGSSGG
SAAAVAAGLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDSAILLGSMAGFDPKD
TTSVDRPVPNYEAAIGGSVKGMKIGIPKEYRLDGMPAEIEKLWMQGAEWLKAAGAELVEVSLPHTKYALPAYYIVAPAEA
SSNLARYDGVRYGTRVNGRNIVEMYENTRAAGFGAEVKRRIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFEQCFDQGVS
AILTPATPSAAFGIGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGSDSQGLPLGLQLIGRPFDEETLFSLGDVIEQS
AGRFTPAKWWA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=40.650406504065, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=23.780487804878, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=454, Percent_Identity=37.0044052863436, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=507, Percent_Identity=25.0493096646943, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=324, Percent_Identity=25.6172839506173, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=324, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=35.8796296296296, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=462, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648113, Length=453, Percent_Identity=40.1766004415011, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24644968, Length=490, Percent_Identity=25.5102040816327, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652985, Length=516, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922090, Length=516, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652981, Length=516, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652983, Length=516, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161078093, Length=525, Percent_Identity=26.0952380952381, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=31.3008130081301, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=31.3008130081301, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=31.3008130081301, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648441, Length=200, Percent_Identity=32, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=200, Percent_Identity=32, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_RHOP2 (Q2IXB7)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486054.1
- ProteinModelPortal:   Q2IXB7
- SMR:   Q2IXB7
- STRING:   Q2IXB7
- GeneID:   3910227
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2438
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   RPAL316058:RPB_2438-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51991; Mature: 51859

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTSLTLAEARDGLVNKSFTAVELTDAHLAAIEAARVLNAYVLETPDQARQMAQAADA
CCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
QIAKGEGGPLAGIPLGIKDLFATKGTRTTACSKILGDFKPPYESTVTSQLWRDGAVLLGK
CEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSCFGPVVNPWRRAGSETKLVPGGSSGGSAAAVAAGLCLGATATDTG
CCCCCEECCCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDSAILLGSMAGFDPK
CCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCC
DTTSVDRPVPNYEAAIGGSVKGMKIGIPKEYRLDGMPAEIEKLWMQGAEWLKAAGAELVE
CCCCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSLPHTKYALPAYYIVAPAEASSNLARYDGVRYGTRVNGRNIVEMYENTRAAGFGAEVKR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCEECCCHHHHHHHCCCCCCCCHHHHH
RIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFEQCFDQGVSAILTPATPSAAFGIGEKGG
HHEEEEHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCCC
ADPVEMYLNDIFTVTVNMAGLPGIAVPAGSDSQGLPLGLQLIGRPFDEETLFSLGDVIEQ
CCHHHHHHHCEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
SAGRFTPAKWWA
HCCCCCCHHCCC
>Mature Secondary Structure 
TDLTSLTLAEARDGLVNKSFTAVELTDAHLAAIEAARVLNAYVLETPDQARQMAQAADA
CCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
QIAKGEGGPLAGIPLGIKDLFATKGTRTTACSKILGDFKPPYESTVTSQLWRDGAVLLGK
CEECCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSCFGPVVNPWRRAGSETKLVPGGSSGGSAAAVAAGLCLGATATDTG
CCCCCEECCCCCCCCCCCCCCCHHHHCCCCCEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPIARTVRDSAILLGSMAGFDPK
CCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCC
DTTSVDRPVPNYEAAIGGSVKGMKIGIPKEYRLDGMPAEIEKLWMQGAEWLKAAGAELVE
CCCCCCCCCCCCHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSLPHTKYALPAYYIVAPAEASSNLARYDGVRYGTRVNGRNIVEMYENTRAAGFGAEVKR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCEECCCHHHHHHHCCCCCCCCHHHHH
RIMIGTYVLSAGYYDAYYLRAQKVRTLIKRDFEQCFDQGVSAILTPATPSAAFGIGEKGG
HHEEEEHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCCC
ADPVEMYLNDIFTVTVNMAGLPGIAVPAGSDSQGLPLGLQLIGRPFDEETLFSLGDVIEQ
CCHHHHHHHCEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
SAGRFTPAKWWA
HCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA