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The map label for this gene is vanXB [H]

Identifier: 86749546

GI number: 86749546

Start: 2783161

End: 2783913

Strand: Direct

Name: vanXB [H]

Synonym: RPB_2426

Alternate gene names: 86749546

Gene position: 2783161-2783913 (Clockwise)

Preceding gene: 86749545

Following gene: 86749547

Centisome position: 52.2

GC content: 66.93

Gene sequence:

>753_bases
ATGATGCGCCGCCTCGCGCTTGCGTGCTTCGGCATGGTGCTGGCGACGGCGGCGCGCGCGCAGCCGCTGCCCGACGATTT
CGTCTATCTGCGCGATCTCGATCCGAGTATCCGGCAGGACATCCGCTATGCGGGCGTCAACAATTTCGTCGGCCGGAAGC
TTGCCGGCTATGACGCCAACGAGTGCATTGTGAAGCGGTCGGTCGGGCTCGCCTTGCAGAAGGTGCAGCGCGAACTCGCG
CCGCAGAACGTCTCGCTGAAGATGCTCGATTGCTACCGCCCGGCGCGGGCGTCGCGCGAGATGGTGGCGTGGGCGCAGGA
CGGCCATGAGACCGCGGCGCAGAAGCGCTACAATCCCGGCTTCAGCAAGAGCGAGCTGTTCCGGCTCGGCTACATCGCGG
AATATTCCGGCCACTCGACCGGGGCGGCGGTCGACGTCACGCTGGTCGATCTGCATGCGCCGACGGGACCTTCGTTCGAT
CCGAATCGGGCCTATGCGGATTGCACCGCGCCGGAAGCGCTGCGGGCCCCGGAGAACAGCGTCGACATGGGAACCGGCTA
TGACTGCTCGGACGCCAAGGCGCACACTTCCGCCAAATCGATCACCGCCGGGCAGCGCCGCTGGCGCAAGCTGCTGGCGG
GAGCGATGGTGCGGCAGGGCTTCGTCAATTTCAGCAAGGAGTGGTGGCACTTCTCGCTGCCGGGCGGAGGCCGACAGGCC
TACGATTTCCCGATCGCGCCCCGGACGCGGTGA

Upstream 100 bases:

>100_bases
ATCGAAGTGATGCTGCCGCGTGGCGCCACCTCGCATACGCTGGCGCGGTTCGTCAAAGGCTTCAAGGCCGCGCGCGAGCG
GATGAAGTGAGCGCCTGAGC

Downstream 100 bases:

>100_bases
TCTCGAGCGGTCGCTGTCGTATTTCGACGGGGGGCCACAACCCGCGAACCGGCGTGATCTTGCGCGCGTTTGTGCTTAAC
ATGCGCGCGAATCCATTTGC

Product: peptidase M15D, vanX D-ala-D-ala dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanXB [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MMRRLALACFGMVLATAARAQPLPDDFVYLRDLDPSIRQDIRYAGVNNFVGRKLAGYDANECIVKRSVGLALQKVQRELA
PQNVSLKMLDCYRPARASREMVAWAQDGHETAAQKRYNPGFSKSELFRLGYIAEYSGHSTGAAVDVTLVDLHAPTGPSFD
PNRAYADCTAPEALRAPENSVDMGTGYDCSDAKAHTSAKSITAGQRRWRKLLAGAMVRQGFVNFSKEWWHFSLPGGGRQA
YDFPIAPRTR

Sequences:

>Translated_250_residues
MMRRLALACFGMVLATAARAQPLPDDFVYLRDLDPSIRQDIRYAGVNNFVGRKLAGYDANECIVKRSVGLALQKVQRELA
PQNVSLKMLDCYRPARASREMVAWAQDGHETAAQKRYNPGFSKSELFRLGYIAEYSGHSTGAAVDVTLVDLHAPTGPSFD
PNRAYADCTAPEALRAPENSVDMGTGYDCSDAKAHTSAKSITAGQRRWRKLLAGAMVRQGFVNFSKEWWHFSLPGGGRQA
YDFPIAPRTR
>Mature_250_residues
MMRRLALACFGMVLATAARAQPLPDDFVYLRDLDPSIRQDIRYAGVNNFVGRKLAGYDANECIVKRSVGLALQKVQRELA
PQNVSLKMLDCYRPARASREMVAWAQDGHETAAQKRYNPGFSKSELFRLGYIAEYSGHSTGAAVDVTLVDLHAPTGPSFD
PNRAYADCTAPEALRAPENSVDMGTGYDCSDAKAHTSAKSITAGQRRWRKLLAGAMVRQGFVNFSKEWWHFSLPGGGRQA
YDFPIAPRTR

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=220, Percent_Identity=26.3636363636364, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 27665; Mature: 27665

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRRLALACFGMVLATAARAQPLPDDFVYLRDLDPSIRQDIRYAGVNNFVGRKLAGYDAN
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCHH
ECIVKRSVGLALQKVQRELAPQNVSLKMLDCYRPARASREMVAWAQDGHETAAQKRYNPG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCC
FSKSELFRLGYIAEYSGHSTGAAVDVTLVDLHAPTGPSFDPNRAYADCTAPEALRAPENS
CCHHHHHHHHEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCEECCCCCHHHCCCCCC
VDMGTGYDCSDAKAHTSAKSITAGQRRWRKLLAGAMVRQGFVNFSKEWWHFSLPGGGRQA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
YDFPIAPRTR
EECCCCCCCC
>Mature Secondary Structure
MMRRLALACFGMVLATAARAQPLPDDFVYLRDLDPSIRQDIRYAGVNNFVGRKLAGYDAN
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHCCCCCCCCHH
ECIVKRSVGLALQKVQRELAPQNVSLKMLDCYRPARASREMVAWAQDGHETAAQKRYNPG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCC
FSKSELFRLGYIAEYSGHSTGAAVDVTLVDLHAPTGPSFDPNRAYADCTAPEALRAPENS
CCHHHHHHHHEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCEECCCCCHHHCCCCCC
VDMGTGYDCSDAKAHTSAKSITAGQRRWRKLLAGAMVRQGFVNFSKEWWHFSLPGGGRQA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
YDFPIAPRTR
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]