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The map label for this gene is thrS [H]

Identifier: 86749289

GI number: 86749289

Start: 2460020

End: 2462005

Strand: Reverse

Name: thrS [H]

Synonym: RPB_2168

Alternate gene names: 86749289

Gene position: 2462005-2460020 (Counterclockwise)

Preceding gene: 86749295

Following gene: 86749288

Centisome position: 46.18

GC content: 65.26

Gene sequence:

>1986_bases
GTGAGCGCCGAACAGGTCACCGTGACCTTCCCGGACGGCAAGACCCGCGACTATCCGCGCGGCACCACCGGGCTCGACAT
CGCCAAGGGAATTTCGCCGTCGCTGGCCAAGCGCACCGTGGCGATGGCGCTGAACGGCGTGCTGACCGACCTCGCCGATC
CGATCGAGGAAAACGCCGCGATCGATTTCGTCGCCCGCGACGATGCCCGCGCGCTGGAGCTGATCCGGCACGATTGCGCC
CATGTGCTGGCCGAAGCGGTGCAGAGCCTGTGGCCGGGCACCCAGGTGACGATCGGCCCGACCATCGAGAACGGGTTCTA
CTACGACTTCTTCCGCAACGAGCCGTTCACGCCTGAGGATTTCGGCGCGATCGAGAAGAAGATGCGCGAGATCATCCAGC
GCGACAAACCCTTCACCAAGGAAATCTGGAGCCGCGACGAGACCAAGAAGGTGTTCGCCGACAATGGCGAGATGTTCAAG
GTCGAGCTGGTGGATGCGATCCCCGCCGACCAGACCATCAAGATCTACAAGCAGGGCGACTGGTTCGACCTGTGCCGCGG
CCCGCACATGACCTCGACCGGCAAGATCGGCACCGCCTTCAAGCTGATGAAGGTGGCCGGCGCGTATTGGCGCGGCGACA
GCAACAACCCGATGCTGACCCGGATCTACGGCACCGCCTTCGCCAAGCAGGACGACCTCGACGCGTATCTGAAGCAGATC
GAGGAAGCCGAGAAGCGCGACCACCGCAAGCTCGGCCGCGAACTCGACCTGTTCCACTTCCAGGAGGAAGGCCCCGGCGT
GGTGTTCTGGCATGCCAAGGGCTGGAGCATCTTCCAGTCGCTGGTCGGCTATATGCGCCGCCGCCTCGCCGGCGACTACG
ACGAGGTCAACGCGCCGCAGATCCTCGACAAGGTGCTGTGGGAGACCTCCGGGCACTGGGAGTGGTACCGCGAGAACATG
TTCGCGGCGCAATCCGCCGGCGAGGACGCCGAGGACAAGCGCTGGTTCGCGCTGAAGCCGATGAACTGCCCGGGCCACGT
CCAGATCTTCAAGCACGGCCTGAAGAGCTATCGCGACCTGCCGCTGCGCATGGCCGAATTCGGCGTGGTGCATCGCTACG
AGCCGTCCGGCGCGATGCATGGGCTGATGCGGGTGCGCGGCTTCACCCAGGACGACGCCCATGTGTTCTGCACCGAGGCG
CAGCTCGCCGACGAGTGCATGAAGATCAACGACCTGATCATGTCGACCTACTCCGACTTCGGCTTCGACGGCGAACTCAC
CGTCAAGCTCTCGACCCGGCCGGACAAGCGCGTCGGCACCGACGAGATGTGGGATCACGCCGAGCGGGTGATGGCCACCG
TGCTCAGCGAGATCAAGGCGCAGGGTAGTAACCGGATCAAGACCGAGATCAATCCGGGCGAAGGCGCGTTCTACGGGCCG
AAATTCGAATACGTGCTGCGCGACGCGATCGGCCGCGACTGGCAATGCGGCACCACGCAGGTCGACTTCAACCTGCCGGA
ACGCTTCGGCGCGTTCTACATCGACGCCGACGGCGCCAAGAAGGCGCCGGTGATGGTGCACCGCGCAATCTGCGGCTCGA
TGGAGCGCTTCATCGGCATCCTGATCGAGCACTTCGCCGGCAATTTCCCGCTGTGGCTGGCGCCGGTGCAGCTGGTGGTC
GCGACCATTACTTCCGAGGGCGATGAGTACGCCAAGCAGGTGGTCGCCGCGGCGCGGCGCGCGGGACTACGCGTCGACAT
CGATTTGCGCAACGAGAAGATCAACCTCAAGGTGCGCGAGCATTCGCTGGCCAAGGTCCCGGCGCTGCTCGTCGTCGGCA
AGAAGGAAGCCGAGACGCATTCGGTCTCGGTGCGACGGCTCGGCAGCGACGGCCAGACGGTGATGCCGACCGCCGAGGCG
ATCGCCGCTTTGGTGGACGAAGCGACGCCGCCGGACGTGCGGCGGATGCGAGCGCAGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGTGTGCAGGAGTGAGCCACAGGAGAACGACATGACCGACAAGACCCCTGCCCCGCCGGCCTCCGGCTTTCAATTCACCC
TCTCCAATCTCAAGCCGGCG

Downstream 100 bases:

>100_bases
GGCGTTCGTGGCGCGGTGAAGCCGCCGCGCCACAATCGTCCGGCGATCGGGATCGATTGCAGGATGACTTCTAATGAGGT
AGTCTGCCGATATTACTCTG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MSAEQVTVTFPDGKTRDYPRGTTGLDIAKGISPSLAKRTVAMALNGVLTDLADPIEENAAIDFVARDDARALELIRHDCA
HVLAEAVQSLWPGTQVTIGPTIENGFYYDFFRNEPFTPEDFGAIEKKMREIIQRDKPFTKEIWSRDETKKVFADNGEMFK
VELVDAIPADQTIKIYKQGDWFDLCRGPHMTSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQDDLDAYLKQI
EEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWSIFQSLVGYMRRRLAGDYDEVNAPQILDKVLWETSGHWEWYRENM
FAAQSAGEDAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPLRMAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEA
QLADECMKINDLIMSTYSDFGFDGELTVKLSTRPDKRVGTDEMWDHAERVMATVLSEIKAQGSNRIKTEINPGEGAFYGP
KFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSMERFIGILIEHFAGNFPLWLAPVQLVV
ATITSEGDEYAKQVVAAARRAGLRVDIDLRNEKINLKVREHSLAKVPALLVVGKKEAETHSVSVRRLGSDGQTVMPTAEA
IAALVDEATPPDVRRMRAQAA

Sequences:

>Translated_661_residues
MSAEQVTVTFPDGKTRDYPRGTTGLDIAKGISPSLAKRTVAMALNGVLTDLADPIEENAAIDFVARDDARALELIRHDCA
HVLAEAVQSLWPGTQVTIGPTIENGFYYDFFRNEPFTPEDFGAIEKKMREIIQRDKPFTKEIWSRDETKKVFADNGEMFK
VELVDAIPADQTIKIYKQGDWFDLCRGPHMTSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQDDLDAYLKQI
EEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWSIFQSLVGYMRRRLAGDYDEVNAPQILDKVLWETSGHWEWYRENM
FAAQSAGEDAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPLRMAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEA
QLADECMKINDLIMSTYSDFGFDGELTVKLSTRPDKRVGTDEMWDHAERVMATVLSEIKAQGSNRIKTEINPGEGAFYGP
KFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSMERFIGILIEHFAGNFPLWLAPVQLVV
ATITSEGDEYAKQVVAAARRAGLRVDIDLRNEKINLKVREHSLAKVPALLVVGKKEAETHSVSVRRLGSDGQTVMPTAEA
IAALVDEATPPDVRRMRAQAA
>Mature_660_residues
SAEQVTVTFPDGKTRDYPRGTTGLDIAKGISPSLAKRTVAMALNGVLTDLADPIEENAAIDFVARDDARALELIRHDCAH
VLAEAVQSLWPGTQVTIGPTIENGFYYDFFRNEPFTPEDFGAIEKKMREIIQRDKPFTKEIWSRDETKKVFADNGEMFKV
ELVDAIPADQTIKIYKQGDWFDLCRGPHMTSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQDDLDAYLKQIE
EAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWSIFQSLVGYMRRRLAGDYDEVNAPQILDKVLWETSGHWEWYRENMF
AAQSAGEDAEDKRWFALKPMNCPGHVQIFKHGLKSYRDLPLRMAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEAQ
LADECMKINDLIMSTYSDFGFDGELTVKLSTRPDKRVGTDEMWDHAERVMATVLSEIKAQGSNRIKTEINPGEGAFYGPK
FEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSMERFIGILIEHFAGNFPLWLAPVQLVVA
TITSEGDEYAKQVVAAARRAGLRVDIDLRNEKINLKVREHSLAKVPALLVVGKKEAETHSVSVRRLGSDGQTVMPTAEAI
AALVDEATPPDVRRMRAQAA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=628, Percent_Identity=41.7197452229299, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI38570111, Length=625, Percent_Identity=40, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=636, Percent_Identity=37.5786163522013, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI1788013, Length=653, Percent_Identity=50.8422664624809, Blast_Score=677, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=628, Percent_Identity=39.171974522293, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=628, Percent_Identity=39.171974522293, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=625, Percent_Identity=40.48, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=35.264483627204, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24583842, Length=646, Percent_Identity=40.8668730650155, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574141, Length=646, Percent_Identity=40.8668730650155, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24583839, Length=646, Percent_Identity=40.8668730650155, Blast_Score=475, Evalue=1e-134,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74404; Mature: 74273

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEQVTVTFPDGKTRDYPRGTTGLDIAKGISPSLAKRTVAMALNGVLTDLADPIEENAA
CCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDFVARDDARALELIRHDCAHVLAEAVQSLWPGTQVTIGPTIENGFYYDFFRNEPFTPED
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEEECCCCCCCHH
FGAIEKKMREIIQRDKPFTKEIWSRDETKKVFADNGEMFKVELVDAIPADQTIKIYKQGD
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHCCCCCCEEEEEEEECCCCCCEEEEEECCC
WFDLCRGPHMTSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQDDLDAYLKQI
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHH
EEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWSIFQSLVGYMRRRLAGDYDEVNAPQ
HHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ILDKVLWETSGHWEWYRENMFAAQSAGEDAEDKRWFALKPMNCPGHVQIFKHGLKSYRDL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
PLRMAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEAQLADECMKINDLIMSTYSDF
CHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
GFDGELTVKLSTRPDKRVGTDEMWDHAERVMATVLSEIKAQGSNRIKTEINPGEGAFYGP
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
KFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSMERFIGI
HHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LIEHFAGNFPLWLAPVQLVVATITSEGDEYAKQVVAAARRAGLRVDIDLRNEKINLKVRE
HHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
HSLAKVPALLVVGKKEAETHSVSVRRLGSDGQTVMPTAEAIAALVDEATPPDVRRMRAQA
CHHHHCCEEEEECCCCCCHHCEEHEEECCCCCEECCHHHHHHHHHHCCCCHHHHHHHHCC
A
C
>Mature Secondary Structure 
SAEQVTVTFPDGKTRDYPRGTTGLDIAKGISPSLAKRTVAMALNGVLTDLADPIEENAA
CCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
IDFVARDDARALELIRHDCAHVLAEAVQSLWPGTQVTIGPTIENGFYYDFFRNEPFTPED
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEEECCCCCCCHH
FGAIEKKMREIIQRDKPFTKEIWSRDETKKVFADNGEMFKVELVDAIPADQTIKIYKQGD
HHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHCCCCCCEEEEEEEECCCCCCEEEEEECCC
WFDLCRGPHMTSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQDDLDAYLKQI
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHH
EEAEKRDHRKLGRELDLFHFQEEGPGVVFWHAKGWSIFQSLVGYMRRRLAGDYDEVNAPQ
HHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ILDKVLWETSGHWEWYRENMFAAQSAGEDAEDKRWFALKPMNCPGHVQIFKHGLKSYRDL
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
PLRMAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEAQLADECMKINDLIMSTYSDF
CHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
GFDGELTVKLSTRPDKRVGTDEMWDHAERVMATVLSEIKAQGSNRIKTEINPGEGAFYGP
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
KFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIDADGAKKAPVMVHRAICGSMERFIGI
HHHHHHHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LIEHFAGNFPLWLAPVQLVVATITSEGDEYAKQVVAAARRAGLRVDIDLRNEKINLKVRE
HHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
HSLAKVPALLVVGKKEAETHSVSVRRLGSDGQTVMPTAEAIAALVDEATPPDVRRMRAQA
CHHHHCCEEEEECCCCCCHHCEEHEEECCCCCEECCHHHHHHHHHHCCCCHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA