Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 86749078

GI number: 86749078

Start: 2222731

End: 2223339

Strand: Reverse

Name: thiE [H]

Synonym: RPB_1955

Alternate gene names: 86749078

Gene position: 2223339-2222731 (Counterclockwise)

Preceding gene: 86749079

Following gene: 86749077

Centisome position: 41.7

GC content: 68.47

Gene sequence:

>609_bases
ATGCCGTATCCTGATCGCTTCTATCCCGTCGTCGACAGCATCGCGTGGGTCAAACGCCTCGCCGCGCTCGGCGTCGGCAC
CGTGCAACTCCGCGCCAAGGACCTCGACGACGGCGCCGCGCTGCAGCTCGTCACCGACGCGCTCGACGCGGTGAAAGGCA
CCAGCGTCAGGCTGATCATCAACGACTACTGGCGCGCCGCGATCGTCGCCGGCGCGCAGCATCTGCATCTCGGCCAGGAA
GACCTCGCGACCGCCGATCTGCACGAGATCCGCGCCGCCGGGCTGACGCTCGGCCTGTCGACCCACGACGACGCCGAACT
CGAAACCGCGCTGGCCGCGCGCCCGGACTACATCGCGCTCGGCCCGATCTTTCCGACCACGCTGAAATCGATGCGCTTCG
CCCCGCAGGGCATCCCGAAGATCACCGACTGGAAGCAGCGCGTCGGCAGCATCCCGCTGGTGGCGATCGGCGGCATCAAG
CTGGAACAGGCCGAAGAGATCTTTGCCGCCGGCGCCGACTCGATCGCGGTGGTGAGCGACGTGACGCAGAACCCCGATCC
AGACGCCCGCGTCCGCGCCTGGCTCGATTTCGTCGCGGAGACGGCGTGA

Upstream 100 bases:

>100_bases
CTCGCGGTCGAAGCCGGCCGCACCGGATTCGAGGCCGGGCTGATGGGCGCCCGCGATTTCGCCTCCCCCTCAACCCCCGT
GATTGGGACCCCGTTCTGGC

Downstream 100 bases:

>100_bases
GCCGCGGCACAAAGGATGTCGCCGTCACCCTGAGGTGCGAGCCGTCTTCGGCTCGCCTCGAAGGGCGACGGCGGGAGCAC
TGAATTCGTTGCGGTCATCC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPYPDRFYPVVDSIAWVKRLAALGVGTVQLRAKDLDDGAALQLVTDALDAVKGTSVRLIINDYWRAAIVAGAQHLHLGQE
DLATADLHEIRAAGLTLGLSTHDDAELETALAARPDYIALGPIFPTTLKSMRFAPQGIPKITDWKQRVGSIPLVAIGGIK
LEQAEEIFAAGADSIAVVSDVTQNPDPDARVRAWLDFVAETA

Sequences:

>Translated_202_residues
MPYPDRFYPVVDSIAWVKRLAALGVGTVQLRAKDLDDGAALQLVTDALDAVKGTSVRLIINDYWRAAIVAGAQHLHLGQE
DLATADLHEIRAAGLTLGLSTHDDAELETALAARPDYIALGPIFPTTLKSMRFAPQGIPKITDWKQRVGSIPLVAIGGIK
LEQAEEIFAAGADSIAVVSDVTQNPDPDARVRAWLDFVAETA
>Mature_201_residues
PYPDRFYPVVDSIAWVKRLAALGVGTVQLRAKDLDDGAALQLVTDALDAVKGTSVRLIINDYWRAAIVAGAQHLHLGQED
LATADLHEIRAAGLTLGLSTHDDAELETALAARPDYIALGPIFPTTLKSMRFAPQGIPKITDWKQRVGSIPLVAIGGIKL
EQAEEIFAAGADSIAVVSDVTQNPDPDARVRAWLDFVAETA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=51.6129032258064, Blast_Score=177, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6325042, Length=202, Percent_Identity=30.1980198019802, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21675; Mature: 21544

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYPDRFYPVVDSIAWVKRLAALGVGTVQLRAKDLDDGAALQLVTDALDAVKGTSVRLII
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
NDYWRAAIVAGAQHLHLGQEDLATADLHEIRAAGLTLGLSTHDDAELETALAARPDYIAL
HHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCCCEEEE
GPIFPTTLKSMRFAPQGIPKITDWKQRVGSIPLVAIGGIKLEQAEEIFAAGADSIAVVSD
CCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEECCEEHHHHHHHHHCCCCCEEEEHH
VTQNPDPDARVRAWLDFVAETA
CCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PYPDRFYPVVDSIAWVKRLAALGVGTVQLRAKDLDDGAALQLVTDALDAVKGTSVRLII
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
NDYWRAAIVAGAQHLHLGQEDLATADLHEIRAAGLTLGLSTHDDAELETALAARPDYIAL
HHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHCCCCEEEE
GPIFPTTLKSMRFAPQGIPKITDWKQRVGSIPLVAIGGIKLEQAEEIFAAGADSIAVVSD
CCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEECCEEHHHHHHHHHCCCCCEEEEHH
VTQNPDPDARVRAWLDFVAETA
CCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]