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The map label for this gene is thiC

Identifier: 86749077

GI number: 86749077

Start: 2220611

End: 2222551

Strand: Reverse

Name: thiC

Synonym: RPB_1954

Alternate gene names: 86749077

Gene position: 2222551-2220611 (Counterclockwise)

Preceding gene: 86749078

Following gene: 86749076

Centisome position: 41.69

GC content: 64.66

Gene sequence:

>1941_bases
ATGAATATCCGCTCCAATCCCGACACCACGCGTCCCGCCGTCACCACCGGCGGCCTGCCCTCCTCGCGCAAGATCTATGC
TGTGCCCGCCACCGCGCCGGATCTGCGCGTGCCGCTGCGCGAGATCATGCTGAGCGAAGGCGCCGGCGAGCCGAACCTGC
CGGTGTACGACACCTCGGGCCCCTACACCGATCCCGGCGTCACCATCGACGTCAACAAGGGCCTGTCGCGCGCCCGCACG
GAGTGGGTCAAGCAGCGCGGCGGCGTCGAGCAATATGAAGGCCGCGACATCAAGCCGGAAGACAACGGCAATGTCGGCGC
AGCCCATGCGGCGAAGTCGTTCACCGCGCATCACCAGCCGCTGCGCGGCGTCGGCGACGCGCCGATCACGCAATACGAAT
TCGCCCGCAAGGGGATCATCACCAAGGAGATGATCTACGTCGCCGAGCGCGAGAATCTCGGCCGCAAGCAGCAACTGGAG
CGCGCCGAAGCCGCGCTGGCCGACGGTGAAAGCTTCGGCGCCGCGGTGCCGGCGTTCATCACCCCGGAATTCGTCCGCGA
CGAGATCGCGCGCGGCCGCGCCATCATCCCGGCCAACATCAATCACGGCGAACTCGAGCCGATGATCATCGGCCGCAACT
TCCTCACCAAGATCAACGCCAACATCGGCAACTCGGCGGTGACCTCGTCGGTCGAGGAGGAAGTCGACAAGATGGTGTGG
GCGATCCGCTGGGGCGCCGACACCGTGATGGACCTCTCCACCGGCCGCAACATCCACACCACCCGCGAATGGATTTTGCG
CAACTCGCCGGTCCCGATCGGCACCGTACCGATCTATCAGGCGCTGGAGAAGTGCGACGGCGATCCGGTCAAGCTCACCT
GGGAGCTGTACAAGGACACGCTGATCGAGCAGGCCGAACAGGGCGTCGATTACTTCACGATCCACGCCGGCGTGCGGCTG
CAATACATCCACCTCACCGCCGATCGCGTCACGGGCATCGTTTCCCGTGGCGGATCGATCATGGCGAAGTGGTGCCTGGC
GCATCACCAGGAGAGCTTCCTCTACACGCATTTCGACGAGATCTGCGACCTGATGCGGAAATACGACGTGTCGTTCTCAT
TGGGCGACGGGCTGCGCCCGGGCTCGATCGCCGACGCCAACGACCGCGCGCAATTCGCCGAACTGGAGACGCTCGGCGAA
CTCACCAAGATCGCCTGGGCCAAGGGCTGCCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGCTGCACAAGATCAAGAT
CAACATGGACAAGCAGCTCAAGGAATGCGGCGAGGCGCCGTTCTATACGCTCGGCCCGCTGACCACCGACATCGCGCCGG
GCTATGATCACATCACCTCCGGCATTGGCGCCGCGATGATCGGCTGGTTCGGCTGCGCGATGCTGTGCTACGTCACGCCG
AAGGAACATCTCGGCCTGCCCGACCGCAACGACGTCAAGACCGGCGTGATCACCTACAAGATCGCCGCCCACGCCGCCGA
CCTCGGCAAGGGCCACCCCGCAGCCCAGTTGCGCGACGACGCGCTGTCCCGCGCCCGCTTCGACTTCCGCTGGCAGGACC
AATTCAACCTCGGCCTCGACCCGGATACGGCGAAAGCCTTCCACGACGAAACCCTGCCCAAGGAAGCCCACAAGGTCGCG
CATTTCTGCTCGATGTGCGGCCCGAAATTCTGCTCGATGAAGATCACGCAAGATGTGCGCGACTACGCGGCGGGATTGGG
CGACAACGAGAAGGCGGCGCTGAACCTCGCCGGCGCCGGCTCGTTCGGCAGCGTCGGCATGACGATGTCCGGCGTCATCG
AAGACGGCATGGCGCAGATGAGCGAGAAGTTTCGGGATATGGGGGAGAAGCTGTATCTCGATGCGGAGAAGGTGAAGGAG
AGCAACAAGGCGCTGTCGTAG

Upstream 100 bases:

>100_bases
CTGAATTCGTTGCGGTCATCCTTCGAGGCGCGCAAGGGCGCGCACCTCAGGATGACGGACCAACCAAGCGGCCCGATAAC
AACACAAACAGGAGGATGGC

Downstream 100 bases:

>100_bases
GGCGGCGCGGCTCTCCACGCCACAAACGTCATGCCCGGGCTCGACCCGGGCATCCATCTGACACAAACGGCCGGGCGAAA
GCCCGGCTGTTTTGTTAATG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MNIRSNPDTTRPAVTTGGLPSSRKIYAVPATAPDLRVPLREIMLSEGAGEPNLPVYDTSGPYTDPGVTIDVNKGLSRART
EWVKQRGGVEQYEGRDIKPEDNGNVGAAHAAKSFTAHHQPLRGVGDAPITQYEFARKGIITKEMIYVAERENLGRKQQLE
RAEAALADGESFGAAVPAFITPEFVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYKDTLIEQAEQGVDYFTIHAGVRL
QYIHLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWAKGCQVMIEGPGHVPLHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLGKGHPAAQLRDDALSRARFDFRWQDQFNLGLDPDTAKAFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAAGLGDNEKAALNLAGAGSFGSVGMTMSGVIEDGMAQMSEKFRDMGEKLYLDAEKVKE
SNKALS

Sequences:

>Translated_646_residues
MNIRSNPDTTRPAVTTGGLPSSRKIYAVPATAPDLRVPLREIMLSEGAGEPNLPVYDTSGPYTDPGVTIDVNKGLSRART
EWVKQRGGVEQYEGRDIKPEDNGNVGAAHAAKSFTAHHQPLRGVGDAPITQYEFARKGIITKEMIYVAERENLGRKQQLE
RAEAALADGESFGAAVPAFITPEFVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYKDTLIEQAEQGVDYFTIHAGVRL
QYIHLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWAKGCQVMIEGPGHVPLHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLGKGHPAAQLRDDALSRARFDFRWQDQFNLGLDPDTAKAFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAAGLGDNEKAALNLAGAGSFGSVGMTMSGVIEDGMAQMSEKFRDMGEKLYLDAEKVKE
SNKALS
>Mature_646_residues
MNIRSNPDTTRPAVTTGGLPSSRKIYAVPATAPDLRVPLREIMLSEGAGEPNLPVYDTSGPYTDPGVTIDVNKGLSRART
EWVKQRGGVEQYEGRDIKPEDNGNVGAAHAAKSFTAHHQPLRGVGDAPITQYEFARKGIITKEMIYVAERENLGRKQQLE
RAEAALADGESFGAAVPAFITPEFVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYKDTLIEQAEQGVDYFTIHAGVRL
QYIHLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWAKGCQVMIEGPGHVPLHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLGKGHPAAQLRDDALSRARFDFRWQDQFNLGLDPDTAKAFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAAGLGDNEKAALNLAGAGSFGSVGMTMSGVIEDGMAQMSEKFRDMGEKLYLDAEKVKE
SNKALS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=631, Percent_Identity=63.2329635499208, Blast_Score=808, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_RHOP2 (Q2IYP8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485573.1
- STRING:   Q2IYP8
- GeneID:   3908033
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1954
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   RPAL316058:RPB_1954-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70806; Mature: 70806

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: BINDING 221-221 BINDING 250-250 BINDING 279-279 BINDING 315-315 BINDING 415-415 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRSNPDTTRPAVTTGGLPSSRKIYAVPATAPDLRVPLREIMLSEGAGEPNLPVYDTSG
CCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCC
PYTDPGVTIDVNKGLSRARTEWVKQRGGVEQYEGRDIKPEDNGNVGAAHAAKSFTAHHQP
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
LRGVGDAPITQYEFARKGIITKEMIYVAERENLGRKQQLERAEAALADGESFGAAVPAFI
CCCCCCCCCHHHHHHHCCCCHHHHHEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHC
TPEFVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
CHHHHHHHHHCCCEEEECCCCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHE
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYKDT
EEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
LIEQAEQGVDYFTIHAGVRLQYIHLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFDE
HHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTKIAWAKGCQVMIEGPGHV
HHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PLHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
CEEEEEECCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECC
KEHLGLPDRNDVKTGVITYKIAAHAADLGKGHPAAQLRDDALSRARFDFRWQDQFNLGLD
HHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCC
PDTAKAFHDETLPKEAHKVAHFCSMCGPKFCSMKITQDVRDYAAGLGDNEKAALNLAGAG
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
SFGSVGMTMSGVIEDGMAQMSEKFRDMGEKLYLDAEKVKESNKALS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHCCCCC
>Mature Secondary Structure
MNIRSNPDTTRPAVTTGGLPSSRKIYAVPATAPDLRVPLREIMLSEGAGEPNLPVYDTSG
CCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEECCCC
PYTDPGVTIDVNKGLSRARTEWVKQRGGVEQYEGRDIKPEDNGNVGAAHAAKSFTAHHQP
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
LRGVGDAPITQYEFARKGIITKEMIYVAERENLGRKQQLERAEAALADGESFGAAVPAFI
CCCCCCCCCHHHHHHHCCCCHHHHHEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHC
TPEFVRDEIARGRAIIPANINHGELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
CHHHHHHHHHCCCEEEECCCCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHE
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCDGDPVKLTWELYKDT
EEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
LIEQAEQGVDYFTIHAGVRLQYIHLTADRVTGIVSRGGSIMAKWCLAHHQESFLYTHFDE
HHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTKIAWAKGCQVMIEGPGHV
HHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
PLHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
CEEEEEECCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECC
KEHLGLPDRNDVKTGVITYKIAAHAADLGKGHPAAQLRDDALSRARFDFRWQDQFNLGLD
HHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCC
PDTAKAFHDETLPKEAHKVAHFCSMCGPKFCSMKITQDVRDYAAGLGDNEKAALNLAGAG
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
SFGSVGMTMSGVIEDGMAQMSEKFRDMGEKLYLDAEKVKESNKALS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA