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The map label for this gene is pheA [H]

Identifier: 86748890

GI number: 86748890

Start: 2025835

End: 2026689

Strand: Reverse

Name: pheA [H]

Synonym: RPB_1767

Alternate gene names: 86748890

Gene position: 2026689-2025835 (Counterclockwise)

Preceding gene: 86748891

Following gene: 86748888

Centisome position: 38.01

GC content: 65.85

Gene sequence:

>855_bases
ATGACCAAGACCATGAAAATCGCATTCCAGGGCGAGCCGGGCGCGAATTCGCATATCGCGATCGGCGACGCCTATCCCAC
CGCCGAGGCGCTGCCCTGCGCCACCTTCGAGGACGCGCTCGCCGCGATCACCTCGGGCGAGGCCGATCTCGGCATGATCC
CGATCGAGAATTCGGTCGCCGGCCGCGTCGCCGACATCCATCATCTGCTGCCGCAATCCGGCCTGTTCATCGTCGGCGAA
TGGTTTCTGCCGATCCGGCATCAGCTCGTCGCGGTGCCCGGCGCCAAGCTCGAAGACATCAAGACGGTCGAGAGCCACGT
CCATGCGCTCGGCCAGTGCCGGCGCATCATCCGCAAATTCGGCATCCGGCCGATCGTCGCCGGCGACACCGCGGGCTCGG
CGCGGATCGTCGCCGAGCGCGGCGACAAGAGCTGTGCGGCGATCTCGTCGCGGCTGGCAGCGAAGATCTACGGCCTCGAC
ATCCTCGCCGAGGACATCGAGGACGAAACCCACAACACCACCCGCTTCGTGATGCTGGCGCGCGAGCCGCGCTGGGCAGC
GCAGGGCTCGGGACCGCTGGTGACGACGTTCGTGTTCCGGGTGCGCAATCTGCCGGCCGCTTTGTACAAGGCGATGGGCG
GCTTCGCCACCAACGGCGTCAACATGACCAAGCTGGAAAGCTACATGGTCGACGGCAATTTCTTCGCGACGCAGTTCTAC
GCCGACGTCGACGGCCACCCCGAAGACCGCAACCTCGCCTTCGCGCTCGACGAGCTGAAGTTCTTCTCCCGCGAATTCAG
GATCGTGGGCGTCTATCCGGGCCACCCGTTCCGGGCGACGTTTACGGAGCGGTAG

Upstream 100 bases:

>100_bases
GGCCGATCTGGAGACCGCACGCCGGGTGCTGGCCGGGTGAGCCTGCCCACTGGCGAAAAATGCTGCGGCTGCTAGAACGC
GCGGCAACACGCAGGAACCC

Downstream 100 bases:

>100_bases
TCGGTCCTAAACGATCCGAAGCCGAGTTCATGACCGAGTTGGCGCAAGACCCCGTCGCAGCGCGACGCGAAGAGAGCTTG
CGGATTTCACAACTTTAGAT

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTKTMKIAFQGEPGANSHIAIGDAYPTAEALPCATFEDALAAITSGEADLGMIPIENSVAGRVADIHHLLPQSGLFIVGE
WFLPIRHQLVAVPGAKLEDIKTVESHVHALGQCRRIIRKFGIRPIVAGDTAGSARIVAERGDKSCAAISSRLAAKIYGLD
ILAEDIEDETHNTTRFVMLAREPRWAAQGSGPLVTTFVFRVRNLPAALYKAMGGFATNGVNMTKLESYMVDGNFFATQFY
ADVDGHPEDRNLAFALDELKFFSREFRIVGVYPGHPFRATFTER

Sequences:

>Translated_284_residues
MTKTMKIAFQGEPGANSHIAIGDAYPTAEALPCATFEDALAAITSGEADLGMIPIENSVAGRVADIHHLLPQSGLFIVGE
WFLPIRHQLVAVPGAKLEDIKTVESHVHALGQCRRIIRKFGIRPIVAGDTAGSARIVAERGDKSCAAISSRLAAKIYGLD
ILAEDIEDETHNTTRFVMLAREPRWAAQGSGPLVTTFVFRVRNLPAALYKAMGGFATNGVNMTKLESYMVDGNFFATQFY
ADVDGHPEDRNLAFALDELKFFSREFRIVGVYPGHPFRATFTER
>Mature_283_residues
TKTMKIAFQGEPGANSHIAIGDAYPTAEALPCATFEDALAAITSGEADLGMIPIENSVAGRVADIHHLLPQSGLFIVGEW
FLPIRHQLVAVPGAKLEDIKTVESHVHALGQCRRIIRKFGIRPIVAGDTAGSARIVAERGDKSCAAISSRLAAKIYGLDI
LAEDIEDETHNTTRFVMLAREPRWAAQGSGPLVTTFVFRVRNLPAALYKAMGGFATNGVNMTKLESYMVDGNFFATQFYA
DVDGHPEDRNLAFALDELKFFSREFRIVGVYPGHPFRATFTER

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=274, Percent_Identity=30.6569343065693, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 31009; Mature: 30878

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00857 PREPHENATE_DEHYDR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTMKIAFQGEPGANSHIAIGDAYPTAEALPCATFEDALAAITSGEADLGMIPIENSVA
CCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
GRVADIHHLLPQSGLFIVGEWFLPIRHQLVAVPGAKLEDIKTVESHVHALGQCRRIIRKF
HHHHHHHHHCCCCCEEEEEEEHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIRPIVAGDTAGSARIVAERGDKSCAAISSRLAAKIYGLDILAEDIEDETHNTTRFVMLA
CCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
REPRWAAQGSGPLVTTFVFRVRNLPAALYKAMGGFATNGVNMTKLESYMVDGNFFATQFY
ECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHEEEECCCEEEEEEE
ADVDGHPEDRNLAFALDELKFFSREFRIVGVYPGHPFRATFTER
ECCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCCCEEECCCCC
>Mature Secondary Structure 
TKTMKIAFQGEPGANSHIAIGDAYPTAEALPCATFEDALAAITSGEADLGMIPIENSVA
CCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCC
GRVADIHHLLPQSGLFIVGEWFLPIRHQLVAVPGAKLEDIKTVESHVHALGQCRRIIRKF
HHHHHHHHHCCCCCEEEEEEEHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GIRPIVAGDTAGSARIVAERGDKSCAAISSRLAAKIYGLDILAEDIEDETHNTTRFVMLA
CCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
REPRWAAQGSGPLVTTFVFRVRNLPAALYKAMGGFATNGVNMTKLESYMVDGNFFATQFY
ECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHEEEECCCEEEEEEE
ADVDGHPEDRNLAFALDELKFFSREFRIVGVYPGHPFRATFTER
ECCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]