Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 86748888

GI number: 86748888

Start: 2022730

End: 2023617

Strand: Reverse

Name: metF [H]

Synonym: RPB_1765

Alternate gene names: 86748888

Gene position: 2023617-2022730 (Counterclockwise)

Preceding gene: 86748890

Following gene: 86748887

Centisome position: 37.95

GC content: 67.12

Gene sequence:

>888_bases
ATGACCGACGTGCTGCAGCACATTCCGGCGATCTCCTTCGAGTTCTTCCCGCCGAAGACCGACGAGATGGAGCGCAATCT
GTGGCAGGCGATCAGCCGGCTGGCGCCGCTGCATCCGCGCTTCGTCTCGGTGACTTACGGCGCCGGCGGCTCGACCCGCG
AGCGCACCCATTCGACCATCGCCCGCATCCTGCGCGAGACCAGCCTGACGCCGGCCGCCCACCTCACCTGCGTCGATGCG
CCCTGCGCCGATGTCGACGACGTGGTCGAGCGCTATCACGAGATCGGCGTCCGCCATATCGTGGCGCTGCGCGGCGATCC
CACCAGCGGCCTCGGCGGCGCCTACACCCCGCATCCGCAGGGTTATCGCAGTTCGGCCGATCTGGTCGCATCGATCAAGC
GCAAGCATCCGGACATCGACGTCACCGTGTCGGCCTATCCGGAGAAACATCCGGAGAGCGCGAGCTTCGACGCCGATCTC
GACATGCTGCAGGCCAAGGTCGATGCCGGGGCGACCCGCGCCATCACCCAGTTCTTCTTCGACAATGATCTGTACTTCCG
CTACCTCGACCGCGTCCGCGCCCGCGGCATCGACATTCCGATCGTGCCCGGCATCCTGCCGGTGCAGAACTTCAAGCAGG
CGTCGAGCTTCGCCACGCGCGCCGGCGCCACCGTGCCGGACTGGCTGGCGAAGCAGTTCGAAGGCCTCGACGACGATCCC
GACACCCGCAAGCTGGTGGCGGCGACGATGGCGGCAGGCCAGGTGCACAAGCTCGCCAAGCACGGCGTCGACACCTTCCA
CTTCTACACCATGAACCGCGCGGACCTGGTGTTCGCGATCAGCCACCTGCTCGGCCTTCGGCCGCAGGCGGCGCAGAAAG
CCGCATGA

Upstream 100 bases:

>100_bases
AGCGGATGGGCGCAGCCTTTCAGCGAATGGCACAGCCTTGCGCGCGGCCGACGATCCGCGGCGCGCCCGTGCGCGAGACC
CTCAGTCTGGATGACCCGAC

Downstream 100 bases:

>100_bases
ACTCCATGACCAAGCCGACATCCCCGAAGCGGACCGAACTGCTGGCGCTGACCCGCGAGCGCATCCTCGTGCTCGACGGC
GCGATGGGCACGATGATCCA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTDVLQHIPAISFEFFPPKTDEMERNLWQAISRLAPLHPRFVSVTYGAGGSTRERTHSTIARILRETSLTPAAHLTCVDA
PCADVDDVVERYHEIGVRHIVALRGDPTSGLGGAYTPHPQGYRSSADLVASIKRKHPDIDVTVSAYPEKHPESASFDADL
DMLQAKVDAGATRAITQFFFDNDLYFRYLDRVRARGIDIPIVPGILPVQNFKQASSFATRAGATVPDWLAKQFEGLDDDP
DTRKLVAATMAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGLRPQAAQKAA

Sequences:

>Translated_295_residues
MTDVLQHIPAISFEFFPPKTDEMERNLWQAISRLAPLHPRFVSVTYGAGGSTRERTHSTIARILRETSLTPAAHLTCVDA
PCADVDDVVERYHEIGVRHIVALRGDPTSGLGGAYTPHPQGYRSSADLVASIKRKHPDIDVTVSAYPEKHPESASFDADL
DMLQAKVDAGATRAITQFFFDNDLYFRYLDRVRARGIDIPIVPGILPVQNFKQASSFATRAGATVPDWLAKQFEGLDDDP
DTRKLVAATMAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGLRPQAAQKAA
>Mature_294_residues
TDVLQHIPAISFEFFPPKTDEMERNLWQAISRLAPLHPRFVSVTYGAGGSTRERTHSTIARILRETSLTPAAHLTCVDAP
CADVDDVVERYHEIGVRHIVALRGDPTSGLGGAYTPHPQGYRSSADLVASIKRKHPDIDVTVSAYPEKHPESASFDADLD
MLQAKVDAGATRAITQFFFDNDLYFRYLDRVRARGIDIPIVPGILPVQNFKQASSFATRAGATVPDWLAKQFEGLDDDPD
TRKLVAATMAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGLRPQAAQKAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=32.6241134751773, Blast_Score=153, Evalue=1e-37,
Organism=Escherichia coli, GI1790377, Length=278, Percent_Identity=53.2374100719424, Blast_Score=293, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI25147441, Length=283, Percent_Identity=28.9752650176678, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=28.9752650176678, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=296, Percent_Identity=34.1216216216216, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321313, Length=292, Percent_Identity=29.7945205479452, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24662658, Length=209, Percent_Identity=27.7511961722488, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32515; Mature: 32384

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLQHIPAISFEFFPPKTDEMERNLWQAISRLAPLHPRFVSVTYGAGGSTRERTHSTI
CCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
ARILRETSLTPAAHLTCVDAPCADVDDVVERYHEIGVRHIVALRGDPTSGLGGAYTPHPQ
HHHHHHHCCCCHHHEEECCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCCC
GYRSSADLVASIKRKHPDIDVTVSAYPEKHPESASFDADLDMLQAKVDAGATRAITQFFF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
DNDLYFRYLDRVRARGIDIPIVPGILPVQNFKQASSFATRAGATVPDWLAKQFEGLDDDP
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
DTRKLVAATMAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGLRPQAAQKAA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
TDVLQHIPAISFEFFPPKTDEMERNLWQAISRLAPLHPRFVSVTYGAGGSTRERTHSTI
CHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHH
ARILRETSLTPAAHLTCVDAPCADVDDVVERYHEIGVRHIVALRGDPTSGLGGAYTPHPQ
HHHHHHHCCCCHHHEEECCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCCC
GYRSSADLVASIKRKHPDIDVTVSAYPEKHPESASFDADLDMLQAKVDAGATRAITQFFF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
DNDLYFRYLDRVRARGIDIPIVPGILPVQNFKQASSFATRAGATVPDWLAKQFEGLDDDP
CCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
DTRKLVAATMAAGQVHKLAKHGVDTFHFYTMNRADLVFAISHLLGLRPQAAQKAA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA