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The map label for this gene is alkJ [H]

Identifier: 86748856

GI number: 86748856

Start: 1978443

End: 1980092

Strand: Reverse

Name: alkJ [H]

Synonym: RPB_1733

Alternate gene names: 86748856

Gene position: 1980092-1978443 (Counterclockwise)

Preceding gene: 86748858

Following gene: 86748852

Centisome position: 37.14

GC content: 68.0

Gene sequence:

>1650_bases
ATGTCGTGGCAAACCACACGTGGAACGGGACGGATGACGCAGATCGAGGGCGAATTCGACTATATCGTGGTGGGCGCCGG
CACTGCCGGCTGCATCGTCGCCAACCGGCTGTCGGCCGATCCGAATTGTCGGGTGCTGCTGCTGGAAGCCGGCGGCCGCG
ACAACTGGATCTGGTTTCACATCCCGGTCGGCTATCTGTTCGCGATCGGCAATCCGCGCTCGGACTGGATGTTCAGGACC
GAGCCGGAGCCCGGCCTCAATGGTCGGTCATTGGCCTATCCGCGCGGCAAGGTGATCGGCGGCTCCTCGGCCATCAACGC
GATGATCTCGATGCGCGGCCAGGCCGCCGATTACGACCACTGGCGCCAGCTCGGCCTGTCCGGTTGGGGCTGGGACGACG
TTCGCCAGGTGTTCCGGCGGCTGGAGGATCACTTCCTCGGCGACAGCGAGCATCACGGTAAAGGCGGCGGCTGGCGGATC
GAGGCGGCGCGGCTGTCCTGGCCGATCCTCGACGCGGTGGCCGACGCCGCCGGCGAGATGGGCATCCCGCGCAGCGCCGA
TTTCAACACCGGCGACAACGAGGGCGTCGGCTATTTCCACGTCAACCAGAAGCGCGGCCGGCGCTGGTCGTCGGCGCGCG
GTTTCCTCAAGCCGGCGCTGCACCGGCCGAATCTGAGGCTGGAAACCGGTGTCGTCACCGACCGCGTGATAGTCGAGAAC
GGCCGAGCCGTCGGCGTGCGGTTCCAGCAAGGCGGCGGCGTGGTCGAGGCGCGGGCGCGGCGCGAGGTGGTGCTGTGCGC
GGGATCGATCGGCTCGGTGCAAGTGCTGCAGCGTTCAGGCATCGGGCCGGCGGAATGGCTCACCCCGCTCGGCATCGATC
CGGTGCTCGATCGCCCGGGCGTCGGCCGCAATCTGCAGGACCATCTGCAGCAGCGCGCGATCTATCGCGTCAGCGGCGGC
CGCACCCTGAACGAGATCTATCACTCGCTGCCGCGGCGCGCCTGGATGGGCATGGACTACGCGCTGCGTCGGCGCGGCCC
GCTGACGATGGCGCCATCGCAGCTCGGCATCTTCACCCGCTCCGATCCGCATCAGGAGCGCGCCAACATCCAGTTTCACG
TCCAGCCATTGTCTCTGGATAAATTCGGCGACCCGCTGCACCGCTTCCCGGCGATCACCGTCAGCGCCTGCAACCTGCGG
CCGACCTCGCGCGGCGAGATCAAGCTGAAATCCACCGCGCTCGACGCCGCCCCCTCGATTGCGCCGCATTATCTGACGAC
CGCCGACGACCGCCGCGTCGCCGCCGACGCGATCCGCTGTACGCGCCGGCTGATGCAACAGCAGGCGCTGGCGAAGTATC
AACCCGAGGAGTATCTGCCCGGCCGCGCGGTGGGCGACGACGACGCCTCGTTGGCGAAAGCCGCCGGCGACATCGGCACC
ACGATCTTCCATCCGGTCGGCACCGCCAAGATGGGCCTCGCCAGCGATCCGATGGCGGTGGTCGACGAACGCTTGCGCCT
GCACGGCCTCGACGGCCTGCGCGTCGTCGACGCCTCGGTGATGCCGACGATCACCTCCGGCAACACCAATACGCCGACCG
CGATGATCGCCGAGAAAGGCGCGACGATGATGCTGGAGGATGGGAAGTAA

Upstream 100 bases:

>100_bases
GAAGTTGGTTTCGTATTCTGATCGGGCAGGCTAGCCGGCCTGCCGCCCGGCGGCAACTGTACACCGCCCCGCCGCGGGGC
TAAGCGTCTGCGCAACCGCG

Downstream 100 bases:

>100_bases
GCGGTGAATTCGATATGCGAATAGCGCCGAGGCAGGTGCACTCGCCGCAATGACGCCGTCATCCTGAGGTGCGCGCTGTT
GCGCGCCTCGAAGGATCGCC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSWQTTRGTGRMTQIEGEFDYIVVGAGTAGCIVANRLSADPNCRVLLLEAGGRDNWIWFHIPVGYLFAIGNPRSDWMFRT
EPEPGLNGRSLAYPRGKVIGGSSAINAMISMRGQAADYDHWRQLGLSGWGWDDVRQVFRRLEDHFLGDSEHHGKGGGWRI
EAARLSWPILDAVADAAGEMGIPRSADFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALHRPNLRLETGVVTDRVIVEN
GRAVGVRFQQGGGVVEARARREVVLCAGSIGSVQVLQRSGIGPAEWLTPLGIDPVLDRPGVGRNLQDHLQQRAIYRVSGG
RTLNEIYHSLPRRAWMGMDYALRRRGPLTMAPSQLGIFTRSDPHQERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLR
PTSRGEIKLKSTALDAAPSIAPHYLTTADDRRVAADAIRCTRRLMQQQALAKYQPEEYLPGRAVGDDDASLAKAAGDIGT
TIFHPVGTAKMGLASDPMAVVDERLRLHGLDGLRVVDASVMPTITSGNTNTPTAMIAEKGATMMLEDGK

Sequences:

>Translated_549_residues
MSWQTTRGTGRMTQIEGEFDYIVVGAGTAGCIVANRLSADPNCRVLLLEAGGRDNWIWFHIPVGYLFAIGNPRSDWMFRT
EPEPGLNGRSLAYPRGKVIGGSSAINAMISMRGQAADYDHWRQLGLSGWGWDDVRQVFRRLEDHFLGDSEHHGKGGGWRI
EAARLSWPILDAVADAAGEMGIPRSADFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALHRPNLRLETGVVTDRVIVEN
GRAVGVRFQQGGGVVEARARREVVLCAGSIGSVQVLQRSGIGPAEWLTPLGIDPVLDRPGVGRNLQDHLQQRAIYRVSGG
RTLNEIYHSLPRRAWMGMDYALRRRGPLTMAPSQLGIFTRSDPHQERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLR
PTSRGEIKLKSTALDAAPSIAPHYLTTADDRRVAADAIRCTRRLMQQQALAKYQPEEYLPGRAVGDDDASLAKAAGDIGT
TIFHPVGTAKMGLASDPMAVVDERLRLHGLDGLRVVDASVMPTITSGNTNTPTAMIAEKGATMMLEDGK
>Mature_548_residues
SWQTTRGTGRMTQIEGEFDYIVVGAGTAGCIVANRLSADPNCRVLLLEAGGRDNWIWFHIPVGYLFAIGNPRSDWMFRTE
PEPGLNGRSLAYPRGKVIGGSSAINAMISMRGQAADYDHWRQLGLSGWGWDDVRQVFRRLEDHFLGDSEHHGKGGGWRIE
AARLSWPILDAVADAAGEMGIPRSADFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALHRPNLRLETGVVTDRVIVENG
RAVGVRFQQGGGVVEARARREVVLCAGSIGSVQVLQRSGIGPAEWLTPLGIDPVLDRPGVGRNLQDHLQQRAIYRVSGGR
TLNEIYHSLPRRAWMGMDYALRRRGPLTMAPSQLGIFTRSDPHQERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRP
TSRGEIKLKSTALDAAPSIAPHYLTTADDRRVAADAIRCTRRLMQQQALAKYQPEEYLPGRAVGDDDASLAKAAGDIGTT
IFHPVGTAKMGLASDPMAVVDERLRLHGLDGLRVVDASVMPTITSGNTNTPTAMIAEKGATMMLEDGK

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=537, Percent_Identity=38.9199255121043, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI1786503, Length=539, Percent_Identity=38.961038961039, Blast_Score=342, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17532301, Length=557, Percent_Identity=32.3159784560144, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24642042, Length=572, Percent_Identity=35.4895104895105, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24642055, Length=585, Percent_Identity=35.3846153846154, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24642048, Length=578, Percent_Identity=34.2560553633218, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45551458, Length=576, Percent_Identity=32.9861111111111, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI18859995, Length=579, Percent_Identity=34.7150259067358, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI45549471, Length=576, Percent_Identity=32.9861111111111, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=33.9754816112084, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=32.7556325823224, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI17137792, Length=559, Percent_Identity=33.4525939177102, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24642051, Length=561, Percent_Identity=31.5508021390374, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24650267, Length=574, Percent_Identity=32.2299651567944, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859993, Length=579, Percent_Identity=32.4697754749568, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24642035, Length=574, Percent_Identity=31.7073170731707, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642037, Length=301, Percent_Identity=36.5448504983389, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24642057, Length=581, Percent_Identity=29.776247848537, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645930, Length=578, Percent_Identity=28.2006920415225, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60116; Mature: 59985

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQTTRGTGRMTQIEGEFDYIVVGAGTAGCIVANRLSADPNCRVLLLEAGGRDNWIWFH
CCCCCCCCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEE
IPVGYLFAIGNPRSDWMFRTEPEPGLNGRSLAYPRGKVIGGSSAINAMISMRGQAADYDH
ECCEEEEEECCCCCCCEEEECCCCCCCCCEECCCCCCEECCHHHHHHHHHHCCCCCCHHH
WRQLGLSGWGWDDVRQVFRRLEDHFLGDSEHHGKGGGWRIEAARLSWPILDAVADAAGEM
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCC
GIPRSADFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALHRPNLRLETGVVTDRVIVEN
CCCCCCCCCCCCCCCEEEEEECHHHCCCCHHHCCHHHHHHCCCCCEEECCCEECEEEEEC
GRAVGVRFQQGGGVVEARARREVVLCAGSIGSVQVLQRSGIGPAEWLTPLGIDPVLDRPG
CCEEEEEEECCCCEEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCC
VGRNLQDHLQQRAIYRVSGGRTLNEIYHSLPRRAWMGMDYALRRRGPLTMAPSQLGIFTR
CCCCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCEEEEC
SDPHQERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGEIKLKSTALDAAPSI
CCCCHHHCCEEEEEEEECHHHCCCHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCC
APHYLTTADDRRVAADAIRCTRRLMQQQALAKYQPEEYLPGRAVGDDDASLAKAAGDIGT
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHCCCCC
TIFHPVGTAKMGLASDPMAVVDERLRLHGLDGLRVVDASVMPTITSGNTNTPTAMIAEKG
CEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEECCCCCCCEEEEECCC
ATMMLEDGK
CEEEEECCC
>Mature Secondary Structure 
SWQTTRGTGRMTQIEGEFDYIVVGAGTAGCIVANRLSADPNCRVLLLEAGGRDNWIWFH
CCCCCCCCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEE
IPVGYLFAIGNPRSDWMFRTEPEPGLNGRSLAYPRGKVIGGSSAINAMISMRGQAADYDH
ECCEEEEEECCCCCCCEEEECCCCCCCCCEECCCCCCEECCHHHHHHHHHHCCCCCCHHH
WRQLGLSGWGWDDVRQVFRRLEDHFLGDSEHHGKGGGWRIEAARLSWPILDAVADAAGEM
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCC
GIPRSADFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALHRPNLRLETGVVTDRVIVEN
CCCCCCCCCCCCCCCEEEEEECHHHCCCCHHHCCHHHHHHCCCCCEEECCCEECEEEEEC
GRAVGVRFQQGGGVVEARARREVVLCAGSIGSVQVLQRSGIGPAEWLTPLGIDPVLDRPG
CCEEEEEEECCCCEEEECCCCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCC
VGRNLQDHLQQRAIYRVSGGRTLNEIYHSLPRRAWMGMDYALRRRGPLTMAPSQLGIFTR
CCCCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCCEEEEC
SDPHQERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLRPTSRGEIKLKSTALDAAPSI
CCCCHHHCCEEEEEEEECHHHCCCHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCCCCC
APHYLTTADDRRVAADAIRCTRRLMQQQALAKYQPEEYLPGRAVGDDDASLAKAAGDIGT
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHCCCCC
TIFHPVGTAKMGLASDPMAVVDERLRLHGLDGLRVVDASVMPTITSGNTNTPTAMIAEKG
CEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEECCCCCCCEEEEECCC
ATMMLEDGK
CEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]