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The map label for this gene is ispH [H]

Identifier: 86748852

GI number: 86748852

Start: 1971758

End: 1972702

Strand: Reverse

Name: ispH [H]

Synonym: RPB_1729

Alternate gene names: 86748852

Gene position: 1972702-1971758 (Counterclockwise)

Preceding gene: 86748856

Following gene: 86748851

Centisome position: 37.0

GC content: 64.76

Gene sequence:

>945_bases
TTGTCTGACGTTCGCCCTCTCGAAGTCTTTCTTGCCCAGCCCCGTGGTTTCTGTGCCGGCGTCGTCCGCGCGATCGAGAT
CGTCGAGCGCGCGCTGGAGAAATACGGCCCGCCGGTCTATGTGCGGCACGAAATCGTGCACAACAAATATGTGGTCGAGA
GCCTGAAGGCGAAGGGCGCGATCTTCGTCGAGGAGCTTTCCGAGGTGCCGCCGAAGGCGGTGACCGTGTTCAGCGCCCAC
GGCGTCGCCCGCAGCATCGAGGAAGAGGCCGCTTCTCGCGGCCTGCCGGTGCTCAACGCCACCTGCCCGCTGGTCACCAA
GGTGCACAACCAGGGCAAGCGCTACACCGCCAAGGGCCGCACCCTGATCCTGATCGGCCATGCCGGCCACCCCGAGGTCG
AGGGAACGATGGGACAGGTTCCCGGCCCGGTGCTGCTGGTGCAAAATCTCGACGATGTCGCCGCGCTGACGCTGCCGTCG
GATACCCCGGTCGCCTACATCACCCAGACCACGCTCAGCGTCGACGACACGCGGGACATCATCGCGGCGCTTTCAAAGCG
ATTCTCGGATATCCAGGGCCCCGACACCCGCGATATCTGCTATGCAACGCAGAACAGACAGTCTGCAGTTCGGGCCATGA
GCAAGCTGGTCGATCTGATCCTGGTGGTCGGCGCCAACAACAGTTCCAACTCCAACCGGCTCCGGGAAATCGGCACCGAA
GCCGGCATTCCGAGTTATCTGATCTCGGACGGCAGCGAGCTCAACCCGGATTGGCTCGAAGGCAAGAAGGCCGTCGGCAT
CACCGCCGGCGCGTCCGCCCCGGAAGTCCTGGTCGACGACGTCATCGAGGCGCTGCGCCGGATCGTTCCGGTCAGCGTGT
CGGTGCTGCCGGGCCGCGAGGAGAACATCGAATTCGTCCTGCCCGCGGAGCTGGTCACCGCCTGA

Upstream 100 bases:

>100_bases
CGACCCTTGCTCTTGCGGGTGTCCTCATACCAGCTAGATCATGGTACGGGAATTGCCCATAATACGTCAGGAGACGGAAC
AATCATGAGTGTGGTGCCCT

Downstream 100 bases:

>100_bases
TCGCCGACGTCCGATCCGCATCGTGATGATTTGTACGAAAGAGAAGAACCCGTAATGGCAATACCGTTTCACAAAGAGCT
GGTGATCGGCGGCTATCTGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSDVRPLEVFLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKYVVESLKAKGAIFVEELSEVPPKAVTVFSAH
GVARSIEEEAASRGLPVLNATCPLVTKVHNQGKRYTAKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNLDDVAALTLPS
DTPVAYITQTTLSVDDTRDIIAALSKRFSDIQGPDTRDICYATQNRQSAVRAMSKLVDLILVVGANNSSNSNRLREIGTE
AGIPSYLISDGSELNPDWLEGKKAVGITAGASAPEVLVDDVIEALRRIVPVSVSVLPGREENIEFVLPAELVTA

Sequences:

>Translated_314_residues
MSDVRPLEVFLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKYVVESLKAKGAIFVEELSEVPPKAVTVFSAH
GVARSIEEEAASRGLPVLNATCPLVTKVHNQGKRYTAKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNLDDVAALTLPS
DTPVAYITQTTLSVDDTRDIIAALSKRFSDIQGPDTRDICYATQNRQSAVRAMSKLVDLILVVGANNSSNSNRLREIGTE
AGIPSYLISDGSELNPDWLEGKKAVGITAGASAPEVLVDDVIEALRRIVPVSVSVLPGREENIEFVLPAELVTA
>Mature_313_residues
SDVRPLEVFLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKYVVESLKAKGAIFVEELSEVPPKAVTVFSAHG
VARSIEEEAASRGLPVLNATCPLVTKVHNQGKRYTAKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNLDDVAALTLPSD
TPVAYITQTTLSVDDTRDIIAALSKRFSDIQGPDTRDICYATQNRQSAVRAMSKLVDLILVVGANNSSNSNRLREIGTEA
GIPSYLISDGSELNPDWLEGKKAVGITAGASAPEVLVDDVIEALRRIVPVSVSVLPGREENIEFVLPAELVTA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=54.8701298701299, Blast_Score=347, Evalue=5e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 33726; Mature: 33595

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVRPLEVFLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKYVVESLKAKGA
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCC
IFVEELSEVPPKAVTVFSAHGVARSIEEEAASRGLPVLNATCPLVTKVHNQGKRYTAKGR
CHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCEEEECCC
TLILIGHAGHPEVEGTMGQVPGPVLLVQNLDDVAALTLPSDTPVAYITQTTLSVDDTRDI
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEEEEECCCHHHHH
IAALSKRFSDIQGPDTRDICYATQNRQSAVRAMSKLVDLILVVGANNSSNSNRLREIGTE
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
AGIPSYLISDGSELNPDWLEGKKAVGITAGASAPEVLVDDVIEALRRIVPVSVSVLPGRE
CCCCHHHHCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
ENIEFVLPAELVTA
CCEEEEECHHHHCC
>Mature Secondary Structure 
SDVRPLEVFLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKYVVESLKAKGA
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCC
IFVEELSEVPPKAVTVFSAHGVARSIEEEAASRGLPVLNATCPLVTKVHNQGKRYTAKGR
CHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCEEEECCC
TLILIGHAGHPEVEGTMGQVPGPVLLVQNLDDVAALTLPSDTPVAYITQTTLSVDDTRDI
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEEEEECCCHHHHH
IAALSKRFSDIQGPDTRDICYATQNRQSAVRAMSKLVDLILVVGANNSSNSNRLREIGTE
HHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
AGIPSYLISDGSELNPDWLEGKKAVGITAGASAPEVLVDDVIEALRRIVPVSVSVLPGRE
CCCCHHHHCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
ENIEFVLPAELVTA
CCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA