Click here to switch to the map view.

The map label for this gene is hpcG [H]

Identifier: 86748831

GI number: 86748831

Start: 1943317

End: 1944123

Strand: Reverse

Name: hpcG [H]

Synonym: RPB_1708

Alternate gene names: 86748831

Gene position: 1944123-1943317 (Counterclockwise)

Preceding gene: 86748844

Following gene: 86748830

Centisome position: 36.46

GC content: 64.31

Gene sequence:

>807_bases
ATGGCGTTGTCGACGAACGAGATTCGCGCCGCAGCCGAACGGCTGCACCAGGCCGAAAAGACCAGAATTCAGATCCGGCA
GCTCTCGCTCGACCATCCGGCGATCGACATCGGCGACGCCTACGCCATCCAGCACGCCTGGATTGATCTCAAGCGCGCCG
AGGGTCGCACAATTCGCGGCCACAAGATCGGGCTGACCTCGAAAGCGATGCAGAGCCAGCTTTCGATCGACGAGCCGGAT
TCCGGCGTGCTGCTCGACGACATGTTCTTCGCCGACGGCGGCACCGTACCCACCGACCATTTCATCGCCACGCGGATCGA
GGCCGAGCTCGCCTTCGTCATCAGGCATCGCCTCGAAGGCCCGAATTGCACGATCTTCGACGTCCTCAACGCCACCGACT
TCGTCGTTCCCGCACTGGAAATTCTCGATACCCGCGTGCAGCGCGTCGATCCGGACACCAAGGCGACGCGCAAGATCTAC
GACACCATCGCCGACAATGCGGCGAATGCCGGCATCGTGCTCGGTGGCCGGCCGATCCGACCGCTCGACACGGATCTGCG
TTGGATCGGCGCGCTGGTGAGCAGGAACGGCCAACTCGAAGAGACCGGGCTCGCCGCCGGCGTGCTCAACCATCCGGCCA
CCGCGGTGGCATGGCTCGCCAACAAGATCGCAGCCTCGGGGCTGGCGCTGGAACCGGGGCAGACCGTGCTGGCAGGATCG
TTCATCCGCGCTATCGAATGCCGCAAGGGCGACACGATTCAGGCCGACTACGGTCCCTACGGCACCGTGAGTTGCTACTT
CGGCTGA

Upstream 100 bases:

>100_bases
GTTTCGGTTTGAGCCCGATCATTTAACATGTTAATCAATTCTCGCGCGAGCGTTTTCGTGGACGCCGGCTCAGGGACGGA
AATCGAGGCGGAAGCCGAAC

Downstream 100 bases:

>100_bases
CCATCTGAGCGAGAGCGACCATGCCGCATTTCACCATCGAATATTCCGCCAATCTGGAGGACCGCGTCGAGATGGCGGCC
GTCGTCGAACTGGTCCGCGG

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MALSTNEIRAAAERLHQAEKTRIQIRQLSLDHPAIDIGDAYAIQHAWIDLKRAEGRTIRGHKIGLTSKAMQSQLSIDEPD
SGVLLDDMFFADGGTVPTDHFIATRIEAELAFVIRHRLEGPNCTIFDVLNATDFVVPALEILDTRVQRVDPDTKATRKIY
DTIADNAANAGIVLGGRPIRPLDTDLRWIGALVSRNGQLEETGLAAGVLNHPATAVAWLANKIAASGLALEPGQTVLAGS
FIRAIECRKGDTIQADYGPYGTVSCYFG

Sequences:

>Translated_268_residues
MALSTNEIRAAAERLHQAEKTRIQIRQLSLDHPAIDIGDAYAIQHAWIDLKRAEGRTIRGHKIGLTSKAMQSQLSIDEPD
SGVLLDDMFFADGGTVPTDHFIATRIEAELAFVIRHRLEGPNCTIFDVLNATDFVVPALEILDTRVQRVDPDTKATRKIY
DTIADNAANAGIVLGGRPIRPLDTDLRWIGALVSRNGQLEETGLAAGVLNHPATAVAWLANKIAASGLALEPGQTVLAGS
FIRAIECRKGDTIQADYGPYGTVSCYFG
>Mature_267_residues
ALSTNEIRAAAERLHQAEKTRIQIRQLSLDHPAIDIGDAYAIQHAWIDLKRAEGRTIRGHKIGLTSKAMQSQLSIDEPDS
GVLLDDMFFADGGTVPTDHFIATRIEAELAFVIRHRLEGPNCTIFDVLNATDFVVPALEILDTRVQRVDPDTKATRKIYD
TIADNAANAGIVLGGRPIRPLDTDLRWIGALVSRNGQLEETGLAAGVLNHPATAVAWLANKIAASGLALEPGQTVLAGSF
IRAIECRKGDTIQADYGPYGTVSCYFG

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=228, Percent_Identity=34.6491228070175, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 29051; Mature: 28920

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSTNEIRAAAERLHQAEKTRIQIRQLSLDHPAIDIGDAYAIQHAWIDLKRAEGRTIRG
CCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCHHHHHHHHEEEECCCCCEECC
HKIGLTSKAMQSQLSIDEPDSGVLLDDMFFADGGTVPTDHFIATRIEAELAFVIRHRLEG
EEECCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PNCTIFDVLNATDFVVPALEILDTRVQRVDPDTKATRKIYDTIADNAANAGIVLGGRPIR
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
PLDTDLRWIGALVSRNGQLEETGLAAGVLNHPATAVAWLANKIAASGLALEPGQTVLAGS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCHHHHH
FIRAIECRKGDTIQADYGPYGTVSCYFG
HHHHHCCCCCCEEEECCCCCCCEEEECC
>Mature Secondary Structure 
ALSTNEIRAAAERLHQAEKTRIQIRQLSLDHPAIDIGDAYAIQHAWIDLKRAEGRTIRG
CCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCHHHHHHHHEEEECCCCCEECC
HKIGLTSKAMQSQLSIDEPDSGVLLDDMFFADGGTVPTDHFIATRIEAELAFVIRHRLEG
EEECCCHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PNCTIFDVLNATDFVVPALEILDTRVQRVDPDTKATRKIYDTIADNAANAGIVLGGRPIR
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
PLDTDLRWIGALVSRNGQLEETGLAAGVLNHPATAVAWLANKIAASGLALEPGQTVLAGS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCHHHHH
FIRAIECRKGDTIQADYGPYGTVSCYFG
HHHHHCCCCCCEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]