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The map label for this gene is glxB [H]

Identifier: 86748263

GI number: 86748263

Start: 1308946

End: 1309842

Strand: Reverse

Name: glxB [H]

Synonym: RPB_1138

Alternate gene names: 86748263

Gene position: 1309842-1308946 (Counterclockwise)

Preceding gene: 86748264

Following gene: 86748262

Centisome position: 24.57

GC content: 66.33

Gene sequence:

>897_bases
ATGTGCGGAATCGTCGGACTATTCCTGAAGAACAAGGCGCTGGAGCCGGCGCTCGGCGACATGCTGTCGAAGATGCTGGT
GACGATGACGGCGCGCGGCCCGGACAGCGCCGGCGTCGCCGTCTACGGCGCCGGTAACAATGATCGCGTCAAGCTGACGC
TGCGCGCCGAGGATGCCGGCGCGGCCGCCGCGCTGGCGAATGAATTGCCGGCGGCGCTCGGCGTGCCGGTCGATGTCAGC
ATCCGCGCCAATCATATCGTGGCGTCGCTGCCGCGCGACGCCGAAGCGGCAGCGCTGCAATGGGTGAAGGCGCGCGCGGG
TCAGATTCAGCTCGTCAGCGTCGGCACCCATATGGAGATCTACAAGGACGTCGGCCTGCCCGAGCACGTCGTCAGCGAAT
TCGCGCTGGCCGGGATGGCGGGCAGCCACGGCATCGGCCACACCCGCATGGCCACCGAATCGGCGGTGACGATCAACGGC
GCGCATCCGTTCTCGACCGGCGAGGACCAGTGCCTGGTGCACAACGGCTCGCTGTCGAACCACAACAGCATCCGCCGCAC
GCTGGTGCGCGAGGGCATGAGCTTCGCGACGCAGAACGACTCGGAAGTGGCTGCCGGCTATCTGACCTGGCGGATGAGCA
AGGGCGACAGCCTGAAGCAGTCGCTGACCTCCAGCCTGGAAGATCTCGACGGCTTCTTCACCTTCGTGGTCGGCACCCGC
AATGGCTTTGCCGTGCTGCGCGACCCGATCGCCTGCAAGCCGGCGATCATGGCCGAATCCGACGACTACGTCGCCTTCGG
CTCGGAATATCGCGCTTTGTCGGTGCTACCCGCGATCGAGAAGGCGCGGGTGTTCGAGCCCGAACCCGCCACCGTCTATG
CCTGGGAACGTTCGTAA

Upstream 100 bases:

>100_bases
TCGGGCCGTCCGGCTATTTTCTTATCAGGAAACTTCTGTTCTCCCCAGTTGACCCGCCGGACGGTTCCCGCCTATGGTCG
CTCCAGCAAGGAGCAATCGG

Downstream 100 bases:

>100_bases
TGCCTGTCTGTGACCTCGCCGTCAGCTCCGTGCGCGAGCTGAACCAAGCCCTCCACCGGCTCACGCCGGACACCAACGAG
ACCGCGTGGGAGATCGACAA

Product: glutamine amidotransferase, class-II

Products: D-Glucosamine 6-phosphate; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MCGIVGLFLKNKALEPALGDMLSKMLVTMTARGPDSAGVAVYGAGNNDRVKLTLRAEDAGAAAALANELPAALGVPVDVS
IRANHIVASLPRDAEAAALQWVKARAGQIQLVSVGTHMEIYKDVGLPEHVVSEFALAGMAGSHGIGHTRMATESAVTING
AHPFSTGEDQCLVHNGSLSNHNSIRRTLVREGMSFATQNDSEVAAGYLTWRMSKGDSLKQSLTSSLEDLDGFFTFVVGTR
NGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVLPAIEKARVFEPEPATVYAWERS

Sequences:

>Translated_298_residues
MCGIVGLFLKNKALEPALGDMLSKMLVTMTARGPDSAGVAVYGAGNNDRVKLTLRAEDAGAAAALANELPAALGVPVDVS
IRANHIVASLPRDAEAAALQWVKARAGQIQLVSVGTHMEIYKDVGLPEHVVSEFALAGMAGSHGIGHTRMATESAVTING
AHPFSTGEDQCLVHNGSLSNHNSIRRTLVREGMSFATQNDSEVAAGYLTWRMSKGDSLKQSLTSSLEDLDGFFTFVVGTR
NGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVLPAIEKARVFEPEPATVYAWERS
>Mature_298_residues
MCGIVGLFLKNKALEPALGDMLSKMLVTMTARGPDSAGVAVYGAGNNDRVKLTLRAEDAGAAAALANELPAALGVPVDVS
IRANHIVASLPRDAEAAALQWVKARAGQIQLVSVGTHMEIYKDVGLPEHVVSEFALAGMAGSHGIGHTRMATESAVTING
AHPFSTGEDQCLVHNGSLSNHNSIRRTLVREGMSFATQNDSEVAAGYLTWRMSKGDSLKQSLTSSLEDLDGFFTFVVGTR
NGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVLPAIEKARVFEPEPATVYAWERS

Specific function: Catalyzes The First Step In Hexosamine Metabolism, Converting Fructose-6p Into Glucosamine-6p Using Glutamine As A Nitrogen Source. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790167, Length=104, Percent_Identity=31.7307692307692, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: 2.6.1.16 [C]

Molecular weight: Translated: 31632; Mature: 31632

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGLFLKNKALEPALGDMLSKMLVTMTARGPDSAGVAVYGAGNNDRVKLTLRAEDAG
CCCEEEEHHHCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCEEEEEEEECCCC
AAAALANELPAALGVPVDVSIRANHIVASLPRDAEAAALQWVKARAGQIQLVSVGTHMEI
HHHHHHHHCCHHHCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCHHH
YKDVGLPEHVVSEFALAGMAGSHGIGHTRMATESAVTINGAHPFSTGEDQCLVHNGSLSN
HHHCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCEEEEECCCCCC
HNSIRRTLVREGMSFATQNDSEVAAGYLTWRMSKGDSLKQSLTSSLEDLDGFFTFVVGTR
HHHHHHHHHHHCCHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECC
NGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVLPAIEKARVFEPEPATVYAWERS
CCCEEECCCCCCCCEEEECCCCEEEECCCCCEEEECCCHHHHEEECCCCCEEEEEECC
>Mature Secondary Structure
MCGIVGLFLKNKALEPALGDMLSKMLVTMTARGPDSAGVAVYGAGNNDRVKLTLRAEDAG
CCCEEEEHHHCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCEEEEEEEECCCC
AAAALANELPAALGVPVDVSIRANHIVASLPRDAEAAALQWVKARAGQIQLVSVGTHMEI
HHHHHHHHCCHHHCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCHHH
YKDVGLPEHVVSEFALAGMAGSHGIGHTRMATESAVTINGAHPFSTGEDQCLVHNGSLSN
HHHCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCEEEEECCCCCC
HNSIRRTLVREGMSFATQNDSEVAAGYLTWRMSKGDSLKQSLTSSLEDLDGFFTFVVGTR
HHHHHHHHHHHCCHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECC
NGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVLPAIEKARVFEPEPATVYAWERS
CCCEEECCCCCCCCEEEECCCCEEEECCCCCEEEECCCHHHHEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1140 [C]

Specific activity: NA

Km value (mM): 0.65 {glutamine}} 2 {fructose} [C]

Substrates: D-Fructose 6-phosphate; L-Glutamine [C]

Specific reaction: D-Fructose 6-phosphate + L-Glutamine --> D-Glucosamine 6-phosphate + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 6-Diazo-5-oxo-L-norleucine; Anticapsin; Azaserine; Dithiodiformamidine; Dithiodinicotinic acid; Iodoacetamide; N-Ethyl maleimide; N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]