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The map label for this gene is ispE

Identifier: 86748211

GI number: 86748211

Start: 1247765

End: 1248655

Strand: Direct

Name: ispE

Synonym: RPB_1086

Alternate gene names: 86748211

Gene position: 1247765-1248655 (Clockwise)

Preceding gene: 86748210

Following gene: 86748212

Centisome position: 23.4

GC content: 70.93

Gene sequence:

>891_bases
TTGCCGACAACGGCGTTGAGCGACCAGGCCCGCGCCAAGGTCAATCTCACTTTGCGAGTCGTGGGCCGCCGCGTCGACGG
CTATCACGACCTCGAAAGCGTGGTGGCCTTCGCCGATTGCGCCGACCGCCTGACGTTGCATCCGGGTGCCGAACTGAGCC
TCGTCACCTCCGGCCCCGGCGCGCAGGATTGCGGCGACACCGCCGACAATCTGGTGCTCAAGGCGACCCGACTGCTCGCC
GAGCGCGTCCCGAAACTGCGCAGCGGCGCCTTCGTCCTCGACAAGCATCTGCCGGTCGCGGCCGGGATCGGTGGCGGCTC
GGCCGACGCCGCCGCGGCGTTGCGGCTGCTCGCCCGCGCCAACGATCTGGCGGCCGACGATCCGCGCGTGGTCGAGGCGG
CGCGTCTCACCGGGGCCGACGTGCCGGTATGCCTGCCGTCCCGGCCCTGCGTCATGACCGGAGTCGGCGATACGCTGACG
CCGTTGCCGCTGCCGCGGCTGCCGGCGGTGATGGTCAATCCGCGCGTGCCGGTGGCGACCAAGGACGTGTTCAAGGCGCT
CGGCCTGCGTGCCGGTCAGCTCAATGTCGGCATTGTCGACGTGCTGAAATCGAAGGGCTGGCCCGCGGTGGATGCGTCCG
TTGCCGACTGGATCGTGGCGGTGAGGCGCGGCACGAACGATCTCGAGGCCCCGGCGCTGAAGGTCGAGCCGATCGTCGGC
GATGTGCTGCGGGCGCTCGCCGCGCTGCCGGGGGTGCGGATGTCGCGGATGTCCGGCTCCGGCGCGACCTGCTTTGCGCT
GTTCGCCAGCGATGAAGATACGAAGGCCGGCGCCGAGGTGCTACGCGCCGCGCATCCCGGCTGGTGGATCCACGCCGGCA
GCCTGAGCTAG

Upstream 100 bases:

>100_bases
GAGGCCAAGCTCGCCAACGGTCTCCCGGAGGACACCTCGTCGGCGGCGTCGGCGGACAAGAAAAAAGACGACGACAAGGG
CGGCTGAGGTGAGTGACGCA

Downstream 100 bases:

>100_bases
TTCCCGCCACGCCGCTGACGGCCGAACCGACCATCTCCGCAGCGTCATGCCGAGCCCACCGCCCGAGACGGCGCGCCTCG
GGGCGCCGCCGCTTGCGTTG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPTTALSDQARAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLHPGAELSLVTSGPGAQDCGDTADNLVLKATRLLA
ERVPKLRSGAFVLDKHLPVAAGIGGGSADAAAALRLLARANDLAADDPRVVEAARLTGADVPVCLPSRPCVMTGVGDTLT
PLPLPRLPAVMVNPRVPVATKDVFKALGLRAGQLNVGIVDVLKSKGWPAVDASVADWIVAVRRGTNDLEAPALKVEPIVG
DVLRALAALPGVRMSRMSGSGATCFALFASDEDTKAGAEVLRAAHPGWWIHAGSLS

Sequences:

>Translated_296_residues
MPTTALSDQARAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLHPGAELSLVTSGPGAQDCGDTADNLVLKATRLLA
ERVPKLRSGAFVLDKHLPVAAGIGGGSADAAAALRLLARANDLAADDPRVVEAARLTGADVPVCLPSRPCVMTGVGDTLT
PLPLPRLPAVMVNPRVPVATKDVFKALGLRAGQLNVGIVDVLKSKGWPAVDASVADWIVAVRRGTNDLEAPALKVEPIVG
DVLRALAALPGVRMSRMSGSGATCFALFASDEDTKAGAEVLRAAHPGWWIHAGSLS
>Mature_295_residues
PTTALSDQARAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLHPGAELSLVTSGPGAQDCGDTADNLVLKATRLLAE
RVPKLRSGAFVLDKHLPVAAGIGGGSADAAAALRLLARANDLAADDPRVVEAARLTGADVPVCLPSRPCVMTGVGDTLTP
LPLPRLPAVMVNPRVPVATKDVFKALGLRAGQLNVGIVDVLKSKGWPAVDASVADWIVAVRRGTNDLEAPALKVEPIVGD
VLRALAALPGVRMSRMSGSGATCFALFASDEDTKAGAEVLRAAHPGWWIHAGSLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=285, Percent_Identity=30.1754385964912, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_RHOP2 (Q2J164)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484707.1
- ProteinModelPortal:   Q2J164
- SMR:   Q2J164
- STRING:   Q2J164
- GeneID:   3910172
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1086
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14608
- BioCyc:   RPAL316058:RPB_1086-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30699; Mature: 30568

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTALSDQARAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLHPGAELSLVTSGPG
CCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCC
AQDCGDTADNLVLKATRLLAERVPKLRSGAFVLDKHLPVAAGIGGGSADAAAALRLLARA
CCCCCCCHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHH
NDLAADDPRVVEAARLTGADVPVCLPSRPCVMTGVGDTLTPLPLPRLPAVMVNPRVPVAT
CCCCCCCCHHHHHHHHCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCH
KDVFKALGLRAGQLNVGIVDVLKSKGWPAVDASVADWIVAVRRGTNDLEAPALKVEPIVG
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECHHHH
DVLRALAALPGVRMSRMSGSGATCFALFASDEDTKAGAEVLRAAHPGWWIHAGSLS
HHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCCCC
>Mature Secondary Structure 
PTTALSDQARAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLHPGAELSLVTSGPG
CCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCC
AQDCGDTADNLVLKATRLLAERVPKLRSGAFVLDKHLPVAAGIGGGSADAAAALRLLARA
CCCCCCCHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHH
NDLAADDPRVVEAARLTGADVPVCLPSRPCVMTGVGDTLTPLPLPRLPAVMVNPRVPVAT
CCCCCCCCHHHHHHHHCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCH
KDVFKALGLRAGQLNVGIVDVLKSKGWPAVDASVADWIVAVRRGTNDLEAPALKVEPIVG
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECHHHH
DVLRALAALPGVRMSRMSGSGATCFALFASDEDTKAGAEVLRAAHPGWWIHAGSLS
HHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA