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The map label for this gene is mutY [H]

Identifier: 86748195

GI number: 86748195

Start: 1229206

End: 1230279

Strand: Direct

Name: mutY [H]

Synonym: RPB_1070

Alternate gene names: 86748195

Gene position: 1229206-1230279 (Clockwise)

Preceding gene: 86748187

Following gene: 86748196

Centisome position: 23.05

GC content: 71.69

Gene sequence:

>1074_bases
GTGATCACAGCGGACGACGACGGCGCCTCCGGCGGCCGCCCGGCGGCGCTGCTGGTCTGGTACGACCGCCATCGCCGCGT
GTTGCCGTGGCGCCCGCCCGCCGGCGTCGCCGCCGACCCCTATGCGGTGTGGCTGTCGGAAATCATGCTGCAGCAAACCA
CCGTCCGCGCGGTCGGCCCGTATTTCGAGAAGTTCATGGCGCGCTGGCCCAGCGTGACGGCGCTCGGCCAAGCCTCGCTC
GACGACGTGCTGCGGATGTGGGCCGGGCTCGGCTACTACTCGCGGGCGCGCAATCTGCACGCCTGCGCGGTCGCGGTGGC
GACGCAGCACGGCGGCCGCTTTCCCGATACGGAAGACGGCCTGCGCGCCTTGCCGGGCGTCGGGCCTTACACCGCGGCGG
CAATCGCGGCGATCGCCTTCGGCCGGCAGACCATGCCGGTCGACGGCAATATCGAGCGCGTGGTGTCGCGGCTCTACGCG
GTCGAAGACGAGATGCCGAAGGCCAAGCCGCGGATCCAGGAACTGGCGCGCACGCTGCTCGGGCCGTCGCGCGCCGGCGA
CAGCGCGCAGGCGCTGATGGATCTCGGCGCCACCATCTGCACGCCGAAGAAGCCGGCCTGCGCGCTGTGCCCGATCGACG
ATGATTGCGCGGCGAGGCGCCGCGGCGACGCGGAGACGTTTCCGCGCAAGGCGCCGAAGAAGACCGGCGCGCTGCGCCGC
GGCGCGGCCTTCGTGGTGATCCGCGGCGATCAGGTGCTGCTCCGCAGCCGCGTCGCCAAAGGCCTGCTCGGCGGCATGAC
CGAGGTGCCGAATTCGGACTGGCTGTCCGATCAGGACGACGCCGCGGCGCGTGCGCAGGCGCCGGCTGTGACAGGCGCCA
CGCGCTGGCATCGCAAGGCAGGAGTCGTCAGCCACGTGTTCACGCATTTCCCGCTCGAGCTCGTGGTCTACACCGCGCAG
GCGCCGGCCGGGACGCACGCGCCGAAGGGCATGCGCTGGGAGAAGATCGCCACGCTCGCCGGCGAAGCGCTGCCCAATCT
GATGCGCAAGGTGATCGCGCATGCGCTCGACTAA

Upstream 100 bases:

>100_bases
CGGGCCGGGCTTGCTCATGACGGGATGCTTTGCGACGATATGGCCCATGAGTCCAGCACAGAGTCCGGCCGGCGCGGCGG
CGCGGCGGGAACCATCCCCG

Downstream 100 bases:

>100_bases
TCACGGTTTTAGCTCGTAGCATTCCGGATGCGGCGGATTTTGTAGATTTCCGCCGATGGTATTTTAAGCCGTCCTTAATC
CGGCTGCTTCTAAGGACGGT

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MITADDDGASGGRPAALLVWYDRHRRVLPWRPPAGVAADPYAVWLSEIMLQQTTVRAVGPYFEKFMARWPSVTALGQASL
DDVLRMWAGLGYYSRARNLHACAVAVATQHGGRFPDTEDGLRALPGVGPYTAAAIAAIAFGRQTMPVDGNIERVVSRLYA
VEDEMPKAKPRIQELARTLLGPSRAGDSAQALMDLGATICTPKKPACALCPIDDDCAARRRGDAETFPRKAPKKTGALRR
GAAFVVIRGDQVLLRSRVAKGLLGGMTEVPNSDWLSDQDDAAARAQAPAVTGATRWHRKAGVVSHVFTHFPLELVVYTAQ
APAGTHAPKGMRWEKIATLAGEALPNLMRKVIAHALD

Sequences:

>Translated_357_residues
MITADDDGASGGRPAALLVWYDRHRRVLPWRPPAGVAADPYAVWLSEIMLQQTTVRAVGPYFEKFMARWPSVTALGQASL
DDVLRMWAGLGYYSRARNLHACAVAVATQHGGRFPDTEDGLRALPGVGPYTAAAIAAIAFGRQTMPVDGNIERVVSRLYA
VEDEMPKAKPRIQELARTLLGPSRAGDSAQALMDLGATICTPKKPACALCPIDDDCAARRRGDAETFPRKAPKKTGALRR
GAAFVVIRGDQVLLRSRVAKGLLGGMTEVPNSDWLSDQDDAAARAQAPAVTGATRWHRKAGVVSHVFTHFPLELVVYTAQ
APAGTHAPKGMRWEKIATLAGEALPNLMRKVIAHALD
>Mature_357_residues
MITADDDGASGGRPAALLVWYDRHRRVLPWRPPAGVAADPYAVWLSEIMLQQTTVRAVGPYFEKFMARWPSVTALGQASL
DDVLRMWAGLGYYSRARNLHACAVAVATQHGGRFPDTEDGLRALPGVGPYTAAAIAAIAFGRQTMPVDGNIERVVSRLYA
VEDEMPKAKPRIQELARTLLGPSRAGDSAQALMDLGATICTPKKPACALCPIDDDCAARRRGDAETFPRKAPKKTGALRR
GAAFVVIRGDQVLLRSRVAKGLLGGMTEVPNSDWLSDQDDAAARAQAPAVTGATRWHRKAGVVSHVFTHFPLELVVYTAQ
APAGTHAPKGMRWEKIATLAGEALPNLMRKVIAHALD

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298654, Length=403, Percent_Identity=37.2208436724566, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI115298652, Length=403, Percent_Identity=37.2208436724566, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI115298650, Length=403, Percent_Identity=37.2208436724566, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI115298648, Length=403, Percent_Identity=37.2208436724566, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI190358497, Length=403, Percent_Identity=37.2208436724566, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI6912520, Length=403, Percent_Identity=37.2208436724566, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1789331, Length=314, Percent_Identity=42.3566878980892, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1787920, Length=187, Percent_Identity=26.2032085561497, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 38472; Mature: 38472

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITADDDGASGGRPAALLVWYDRHRRVLPWRPPAGVAADPYAVWLSEIMLQQTTVRAVGP
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YFEKFMARWPSVTALGQASLDDVLRMWAGLGYYSRARNLHACAVAVATQHGGRFPDTEDG
HHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHH
LRALPGVGPYTAAAIAAIAFGRQTMPVDGNIERVVSRLYAVEDEMPKAKPRIQELARTLL
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
GPSRAGDSAQALMDLGATICTPKKPACALCPIDDDCAARRRGDAETFPRKAPKKTGALRR
CCCCCCHHHHHHHHCCCHHCCCCCCCEEECCCCCHHHHHHCCCHHHCCCCCCHHHHHHHC
GAAFVVIRGDQVLLRSRVAKGLLGGMTEVPNSDWLSDQDDAAARAQAPAVTGATRWHRKA
CCEEEEECCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
GVVSHVFTHFPLELVVYTAQAPAGTHAPKGMRWEKIATLAGEALPNLMRKVIAHALD
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITADDDGASGGRPAALLVWYDRHRRVLPWRPPAGVAADPYAVWLSEIMLQQTTVRAVGP
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YFEKFMARWPSVTALGQASLDDVLRMWAGLGYYSRARNLHACAVAVATQHGGRFPDTEDG
HHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCHHH
LRALPGVGPYTAAAIAAIAFGRQTMPVDGNIERVVSRLYAVEDEMPKAKPRIQELARTLL
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
GPSRAGDSAQALMDLGATICTPKKPACALCPIDDDCAARRRGDAETFPRKAPKKTGALRR
CCCCCCHHHHHHHHCCCHHCCCCCCCEEECCCCCHHHHHHCCCHHHCCCCCCHHHHHHHC
GAAFVVIRGDQVLLRSRVAKGLLGGMTEVPNSDWLSDQDDAAARAQAPAVTGATRWHRKA
CCEEEEECCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
GVVSHVFTHFPLELVVYTAQAPAGTHAPKGMRWEKIATLAGEALPNLMRKVIAHALD
HHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]