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The map label for this gene is cysD [H]

Identifier: 86748168

GI number: 86748168

Start: 1199864

End: 1200739

Strand: Reverse

Name: cysD [H]

Synonym: RPB_1043

Alternate gene names: 86748168

Gene position: 1200739-1199864 (Counterclockwise)

Preceding gene: 86748174

Following gene: 86748167

Centisome position: 22.52

GC content: 66.1

Gene sequence:

>876_bases
ATGACCCTCGCCGCCCGCGACCCCCTCGCCGCCAAGCCGGTGTCGTTCGCCGATCTCGACCCGCGCGAACAGCTCCGCCC
GATGGATCATCTCGACGCGCTGGAAGCGCAGAGCATCTACATATTCCGTGAAGGTTTTGCGCGGCTGAAGAAGCTGGCAC
TGCTGTGGTCGCTCGGCAAAGATTCCAACGTGATGATCTGGCTGGCGCGCAAGGCGTTCTTCGGCAAGGTGCCGTTCCCG
GCGCTGCACGTCGACACCGGCAAGAAGTTTCCTGAGATGTACGCCTTCCGCGAACACTACGCGAAGGAGTGGGATCTCGA
TTTGCGCGTCGATCCCTGCCCGCCGATCGACAGCGTCGATCCGACCCTGCCGCCGGCGGCGCGCTCGGCGGCGCGCAAGA
CCGAAGGCTTGAAGCTGGCGCTGGCCAAATACGGCTTCGACGGACTGATCGCCGGCATCCGCCGCGACGAGGAGGCGACC
CGCGCCAAAGAACGCGTGTTCTCGCCGCGCGGCACCGAGGGCGGCTGGGACGTGCGCGATCAGCCGCCGGAATTCTGGGA
CCAGTTCAACGCCTCGCCGCCGCCCGGCGCTCACTTGCGTATTCACCCCATCCTGCATTGGACCGAGGCCGACATCTGGG
CCTACACCAAGCGCGAGAACATCCCGATCATCCCGCTGTATCTGGCCAAGGACGGCAAGCGCTATCGCTCGCTCGGCGAC
CAGGACATCACCTTCCCGGTGGCGTCGCACGCCTCGTCGATCGACGAGATCCTGCACGAATTGCAGACCACCAAGGTGCC
GGAGCGCGCCGGCCGCGCGCTCGACCACGAGACCGAGGACGCGTTCGAGCGGCTTCGCGTCGCCGGTTATCTGTGA

Upstream 100 bases:

>100_bases
CGACAGAGATGTTCGTTCTCCTGACGACCCCAGCGACGTCGCGCGGCGCCACCACGACGGGTCCGCGGCGATGCGGATCA
GCCAATTCCGGGAGCTGTGA

Downstream 100 bases:

>100_bases
GCTGTGACTTCGCACCAAGTGATGTCCGACAATGAGTGACGCCATGACCATCGCCGTCCCGCCCGCCACGCTCTCCACGC
CGAACGGCACCACGCGCCCC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTLAARDPLAAKPVSFADLDPREQLRPMDHLDALEAQSIYIFREGFARLKKLALLWSLGKDSNVMIWLARKAFFGKVPFP
ALHVDTGKKFPEMYAFREHYAKEWDLDLRVDPCPPIDSVDPTLPPAARSAARKTEGLKLALAKYGFDGLIAGIRRDEEAT
RAKERVFSPRGTEGGWDVRDQPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGD
QDITFPVASHASSIDEILHELQTTKVPERAGRALDHETEDAFERLRVAGYL

Sequences:

>Translated_291_residues
MTLAARDPLAAKPVSFADLDPREQLRPMDHLDALEAQSIYIFREGFARLKKLALLWSLGKDSNVMIWLARKAFFGKVPFP
ALHVDTGKKFPEMYAFREHYAKEWDLDLRVDPCPPIDSVDPTLPPAARSAARKTEGLKLALAKYGFDGLIAGIRRDEEAT
RAKERVFSPRGTEGGWDVRDQPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGD
QDITFPVASHASSIDEILHELQTTKVPERAGRALDHETEDAFERLRVAGYL
>Mature_290_residues
TLAARDPLAAKPVSFADLDPREQLRPMDHLDALEAQSIYIFREGFARLKKLALLWSLGKDSNVMIWLARKAFFGKVPFPA
LHVDTGKKFPEMYAFREHYAKEWDLDLRVDPCPPIDSVDPTLPPAARSAARKTEGLKLALAKYGFDGLIAGIRRDEEATR
AKERVFSPRGTEGGWDVRDQPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGDQ
DITFPVASHASSIDEILHELQTTKVPERAGRALDHETEDAFERLRVAGYL

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=41.1184210526316, Blast_Score=211, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 33034; Mature: 32903

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAARDPLAAKPVSFADLDPREQLRPMDHLDALEAQSIYIFREGFARLKKLALLWSLGK
CCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCC
DSNVMIWLARKAFFGKVPFPALHVDTGKKFPEMYAFREHYAKEWDLDLRVDPCPPIDSVD
CCCEEEEEECHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
PTLPPAARSAARKTEGLKLALAKYGFDGLIAGIRRDEEATRAKERVFSPRGTEGGWDVRD
CCCCHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
QPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGD
CCHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHCCC
QDITFPVASHASSIDEILHELQTTKVPERAGRALDHETEDAFERLRVAGYL
CCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAARDPLAAKPVSFADLDPREQLRPMDHLDALEAQSIYIFREGFARLKKLALLWSLGK
CCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCC
DSNVMIWLARKAFFGKVPFPALHVDTGKKFPEMYAFREHYAKEWDLDLRVDPCPPIDSVD
CCCEEEEEECHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
PTLPPAARSAARKTEGLKLALAKYGFDGLIAGIRRDEEATRAKERVFSPRGTEGGWDVRD
CCCCHHHHHHHHHHCCCEEEHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
QPPEFWDQFNASPPPGAHLRIHPILHWTEADIWAYTKRENIPIIPLYLAKDGKRYRSLGD
CCHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHCCC
QDITFPVASHASSIDEILHELQTTKVPERAGRALDHETEDAFERLRVAGYL
CCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA