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The map label for this gene is ybfF [H]

Identifier: 86748174

GI number: 86748174

Start: 1205940

End: 1206797

Strand: Reverse

Name: ybfF [H]

Synonym: RPB_1049

Alternate gene names: 86748174

Gene position: 1206797-1205940 (Counterclockwise)

Preceding gene: 86748176

Following gene: 86748168

Centisome position: 22.63

GC content: 69.0

Gene sequence:

>858_bases
ATGCAGAATCCGGAAAGTCGCTTCTATCGGTCGCACGGGCTGCGGCTGCATTACGCCGATTGGGGCAACGACACCGCGCC
GCCGCTGCTGCTGATCCACGGCGGCGCCGACCATGCGCGCAGCTGGGATCACCTGGCGCGATCGCTGCGGGCGGATTTCC
ACGTCGTCGCGCCGGATCTGCGCGGCCACGGCGATTCCGACTGGACGCTCGGCGGCAGCTACAGCCTGCCGGAATATGTC
TACGACCTCACCCGGCTGCCGGCGTTCGAAGGCCGCGGGCCGATCACGATCGTCGGCCATTCGATGGGCGGCATGGTGAG
CCTGATCTATGCCGGCACCTTTCCCGAGCAGGTCGCGCGGCTGGTCGTGCTCGACGGCGTCACCGTGAGGCCGGATGCGG
CGAAGCCGCCGGTGCACGAGCGCACCCGCAAATGGATCGGCCAGCTCGAAGCGCTCGAGAACCGCGAGCCGCGGCGCTAT
CGCGACATCGCCGAGGCGGCCGCGCAGATGCAGGCGCACAACAAGCGGCTGGCGCCGGAGCTGGCGCTGCATCTGGCCAC
TTACGGCGTGAAGCAGAACGACGACGGCAGCTACTCCTGGAAGTTCGATCCGCATCAGCGGGTGATGGCGCCGCACCGGC
TGTGGCCGGACGATCACGTCGCGCTGTGGGGCCGCATCACCTGCCCGACGCTGCTGCTGTTCGCCGGCGAGAGTTTTCTC
TCCGATGCGGCCGAAGCGGGGATCGCCGATCATTTCCCACAGGCGCGCGCCGAGACCGTCGCCGGCGCCGGGCACTGGCT
GCAGCACGACAGGCCCGACGAGGTGCTGCGGCTGATCCGCGACTTCCTGCTCGTGTAG

Upstream 100 bases:

>100_bases
TGCAACCCATAGTCGCCTGAGCAAGCCTGTTGTTGCGCCACCACGCGCGCGTCATCGCGCCCCGCGCGCGACGGCCCGGC
AGATTCATTCGCGAGAGACC

Downstream 100 bases:

>100_bases
TTCCGCGGATTGGTCAGCGATCGGCGAAAATCCGGTAGCGCTGCGGCTGCAGGCCGATGCGGTCGCCGACCTTGAGTGAG
TGGTCGCGCGGCGCGTCGAT

Product: Alpha/beta hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQNPESRFYRSHGLRLHYADWGNDTAPPLLLIHGGADHARSWDHLARSLRADFHVVAPDLRGHGDSDWTLGGSYSLPEYV
YDLTRLPAFEGRGPITIVGHSMGGMVSLIYAGTFPEQVARLVVLDGVTVRPDAAKPPVHERTRKWIGQLEALENREPRRY
RDIAEAAAQMQAHNKRLAPELALHLATYGVKQNDDGSYSWKFDPHQRVMAPHRLWPDDHVALWGRITCPTLLLFAGESFL
SDAAEAGIADHFPQARAETVAGAGHWLQHDRPDEVLRLIRDFLLV

Sequences:

>Translated_285_residues
MQNPESRFYRSHGLRLHYADWGNDTAPPLLLIHGGADHARSWDHLARSLRADFHVVAPDLRGHGDSDWTLGGSYSLPEYV
YDLTRLPAFEGRGPITIVGHSMGGMVSLIYAGTFPEQVARLVVLDGVTVRPDAAKPPVHERTRKWIGQLEALENREPRRY
RDIAEAAAQMQAHNKRLAPELALHLATYGVKQNDDGSYSWKFDPHQRVMAPHRLWPDDHVALWGRITCPTLLLFAGESFL
SDAAEAGIADHFPQARAETVAGAGHWLQHDRPDEVLRLIRDFLLV
>Mature_285_residues
MQNPESRFYRSHGLRLHYADWGNDTAPPLLLIHGGADHARSWDHLARSLRADFHVVAPDLRGHGDSDWTLGGSYSLPEYV
YDLTRLPAFEGRGPITIVGHSMGGMVSLIYAGTFPEQVARLVVLDGVTVRPDAAKPPVHERTRKWIGQLEALENREPRRY
RDIAEAAAQMQAHNKRLAPELALHLATYGVKQNDDGSYSWKFDPHQRVMAPHRLWPDDHVALWGRITCPTLLLFAGESFL
SDAAEAGIADHFPQARAETVAGAGHWLQHDRPDEVLRLIRDFLLV

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=298, Percent_Identity=24.1610738255034, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI23200008, Length=271, Percent_Identity=29.1512915129151, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI23200012, Length=271, Percent_Identity=29.1512915129151, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI18777795, Length=179, Percent_Identity=30.1675977653631, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI218777840, Length=286, Percent_Identity=25.5244755244755, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI13376164, Length=286, Percent_Identity=25.5244755244755, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786902, Length=261, Percent_Identity=28.735632183908, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24655874, Length=279, Percent_Identity=24.7311827956989, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356953, Length=273, Percent_Identity=26.3736263736264, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 32001; Mature: 32001

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPESRFYRSHGLRLHYADWGNDTAPPLLLIHGGADHARSWDHLARSLRADFHVVAPDL
CCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCC
RGHGDSDWTLGGSYSLPEYVYDLTRLPAFEGRGPITIVGHSMGGMVSLIYAGTFPEQVAR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEHHHCCCCHHHHHH
LVVLDGVTVRPDAAKPPVHERTRKWIGQLEALENREPRRYRDIAEAAAQMQAHNKRLAPE
HHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LALHLATYGVKQNDDGSYSWKFDPHQRVMAPHRLWPDDHVALWGRITCPTLLLFAGESFL
HHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHCHHHH
SDAAEAGIADHFPQARAETVAGAGHWLQHDRPDEVLRLIRDFLLV
HHHHHCCHHHHCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNPESRFYRSHGLRLHYADWGNDTAPPLLLIHGGADHARSWDHLARSLRADFHVVAPDL
CCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCC
RGHGDSDWTLGGSYSLPEYVYDLTRLPAFEGRGPITIVGHSMGGMVSLIYAGTFPEQVAR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEHHHCCCCHHHHHH
LVVLDGVTVRPDAAKPPVHERTRKWIGQLEALENREPRRYRDIAEAAAQMQAHNKRLAPE
HHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LALHLATYGVKQNDDGSYSWKFDPHQRVMAPHRLWPDDHVALWGRITCPTLLLFAGESFL
HHHHHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHCHHHH
SDAAEAGIADHFPQARAETVAGAGHWLQHDRPDEVLRLIRDFLLV
HHHHHCCHHHHCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]