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The map label for this gene is 86748135

Identifier: 86748135

GI number: 86748135

Start: 1157846

End: 1159168

Strand: Direct

Name: 86748135

Synonym: RPB_1010

Alternate gene names: NA

Gene position: 1157846-1159168 (Clockwise)

Preceding gene: 86748134

Following gene: 86748139

Centisome position: 21.72

GC content: 67.8

Gene sequence:

>1323_bases
ATGGACGCCGCGGTGCCGAGCCATCTCGTCGATCCGCCGGCGATCGTCCGGGACGCCGCCGATCTGCGGCCGGCGGGGCG
CGGCGGCCAGTTGCTGCCGCCCGGCTATCTCGGCACGCGGGGCAGCCAGATCGTCGACGTGACCGGCCGGCCGGTGCGGA
TCGCCTCGATCGGCTGGAACGGCACCGAGGGCCCGCCCGGCGCAGCACCCTCGGGGATCTGGAAGGTCAGCTACCGGACC
GTTCTCGACTCGATCGTCGCCGCGGGCTTCAACACCGTGCGGATTCCATGGACGGATATCGGCCTCGACACGCCGCTGAA
CGGCTACAGCGACCGGCTCGGCTGGATCAACACCACGCTCAATCCCGACCTGCTGGCATCCGACACGCCCGACGCCAACG
GGCGCTATCGCTACGTCACCACGCTGGTGGCGTTTCAGCGCATCGTCGACTACGCCGGCGACATCGGCCTGAAGGTGATT
TTCAATCACCACACCAATCAGGGCACCGCGGGGCAGCAGCGCAACGGGCTGTGGTTCGATCTCGGCCCAGGCACCGACAA
CACCGACGGCATCAAGCCGGGCCGGTTCACCGCGCAGGACTTCAAGCAGAACTGGCTGCGGGTGGCGCGGACCTTCGCCG
GCAATCCGACCGTGATCGGCTACGATCTGCACAACGAGCCCAACGGCGACCGCGGCGCCATCACCTGGGGCGGCGGCGGG
CCGACCGACATCAAGGCGATGTGCGAGGACGTCGGCTCGGCGATCCAGGACGTCAGCCCCGACGTGCTGATCATCTGCGA
GGGGCCGGAGACCTACAAGCCGCCGCCGGAATCGTCGGGGATGGACCCGCGCCACGCCGCGCCCGCGGGCAATCTCACCG
CGGCGGGCGCCAATCCGGTGCGGCTCAAGATCCCGCACAAGCTGGTGTATTCGATCCATGAATATCCGGAGGAGATCGCC
GACACCAAGCGCTGGGGCATTCCGGAGACCGGCAAGGGCTTCATCGACCGGATGAACACCACCTGGGGCTATCTGGTGCG
CGACGACATCGCGCCGGTGTGGATCGGCGAGATGGGCGCATCATTGCGGACGCCCGAGACGCGCGAATGGGCGCGCAATC
TGATCGACTACATGAACGGCAAATACGGCAGCGAGGGCGGCCCGGCCTTTTCGGGCGATCAGCAGCCGATCAGCGGCAGC
TGGTGGCTGATCGGTCCGTCGAACGATCCGCCCTATGGGCTGCAGACGGACTGGGGCGTCGGCCACTATCGACCGGACCA
GATCGCGATCACCGACCAGATGCTGTTTCGGCCGCGCAAGTAG

Upstream 100 bases:

>100_bases
GCCGGGCGGTTCTTCACCTTCGCGATTTTCCTGGTGGTGGTGGCGACGCTGTTCCGGCGGACGGATGCGCCGCCCGACTA
TGTGAGCTGAGGAGCGCGAC

Downstream 100 bases:

>100_bases
AGCGCTTCATCGCTTGATTGAAGCAACGCGGCATTCTCGCGTCCGGAGTCGTCATCCTGAGAGCCTGCCCCGGACTTGAT
CCGGGGTGCGAGCGCAGCGA

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Cellulase E1; Endo-1,4-beta-glucanase E1; Endocellulase E1 [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MDAAVPSHLVDPPAIVRDAADLRPAGRGGQLLPPGYLGTRGSQIVDVTGRPVRIASIGWNGTEGPPGAAPSGIWKVSYRT
VLDSIVAAGFNTVRIPWTDIGLDTPLNGYSDRLGWINTTLNPDLLASDTPDANGRYRYVTTLVAFQRIVDYAGDIGLKVI
FNHHTNQGTAGQQRNGLWFDLGPGTDNTDGIKPGRFTAQDFKQNWLRVARTFAGNPTVIGYDLHNEPNGDRGAITWGGGG
PTDIKAMCEDVGSAIQDVSPDVLIICEGPETYKPPPESSGMDPRHAAPAGNLTAAGANPVRLKIPHKLVYSIHEYPEEIA
DTKRWGIPETGKGFIDRMNTTWGYLVRDDIAPVWIGEMGASLRTPETREWARNLIDYMNGKYGSEGGPAFSGDQQPISGS
WWLIGPSNDPPYGLQTDWGVGHYRPDQIAITDQMLFRPRK

Sequences:

>Translated_440_residues
MDAAVPSHLVDPPAIVRDAADLRPAGRGGQLLPPGYLGTRGSQIVDVTGRPVRIASIGWNGTEGPPGAAPSGIWKVSYRT
VLDSIVAAGFNTVRIPWTDIGLDTPLNGYSDRLGWINTTLNPDLLASDTPDANGRYRYVTTLVAFQRIVDYAGDIGLKVI
FNHHTNQGTAGQQRNGLWFDLGPGTDNTDGIKPGRFTAQDFKQNWLRVARTFAGNPTVIGYDLHNEPNGDRGAITWGGGG
PTDIKAMCEDVGSAIQDVSPDVLIICEGPETYKPPPESSGMDPRHAAPAGNLTAAGANPVRLKIPHKLVYSIHEYPEEIA
DTKRWGIPETGKGFIDRMNTTWGYLVRDDIAPVWIGEMGASLRTPETREWARNLIDYMNGKYGSEGGPAFSGDQQPISGS
WWLIGPSNDPPYGLQTDWGVGHYRPDQIAITDQMLFRPRK
>Mature_440_residues
MDAAVPSHLVDPPAIVRDAADLRPAGRGGQLLPPGYLGTRGSQIVDVTGRPVRIASIGWNGTEGPPGAAPSGIWKVSYRT
VLDSIVAAGFNTVRIPWTDIGLDTPLNGYSDRLGWINTTLNPDLLASDTPDANGRYRYVTTLVAFQRIVDYAGDIGLKVI
FNHHTNQGTAGQQRNGLWFDLGPGTDNTDGIKPGRFTAQDFKQNWLRVARTFAGNPTVIGYDLHNEPNGDRGAITWGGGG
PTDIKAMCEDVGSAIQDVSPDVLIICEGPETYKPPPESSGMDPRHAAPAGNLTAAGANPVRLKIPHKLVYSIHEYPEEIA
DTKRWGIPETGKGFIDRMNTTWGYLVRDDIAPVWIGEMGASLRTPETREWARNLIDYMNGKYGSEGGPAFSGDQQPISGS
WWLIGPSNDPPYGLQTDWGVGHYRPDQIAITDQMLFRPRK

Specific function: Has a very high specific activity on carboxymethylcellulose [H]

COG id: COG2730

COG function: function code G; Endoglucanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CBM2 (carbohydrate binding type-2) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008965
- InterPro:   IPR012291
- InterPro:   IPR001919
- InterPro:   IPR001547
- InterPro:   IPR018087
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00553 CBM_2; PF00150 Cellulase [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 47776; Mature: 47776

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAVPSHLVDPPAIVRDAADLRPAGRGGQLLPPGYLGTRGSQIVDVTGRPVRIASIGWN
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCC
GTEGPPGAAPSGIWKVSYRTVLDSIVAAGFNTVRIPWTDIGLDTPLNGYSDRLGWINTTL
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCC
NPDLLASDTPDANGRYRYVTTLVAFQRIVDYAGDIGLKVIFNHHTNQGTAGQQRNGLWFD
CCCEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEE
LGPGTDNTDGIKPGRFTAQDFKQNWLRVARTFAGNPTVIGYDLHNEPNGDRGAITWGGGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCC
PTDIKAMCEDVGSAIQDVSPDVLIICEGPETYKPPPESSGMDPRHAAPAGNLTAAGANPV
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCE
RLKIPHKLVYSIHEYPEEIADTKRWGIPETGKGFIDRMNTTWGYLVRDDIAPVWIGEMGA
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEECCCCCEEHHHCCC
SLRTPETREWARNLIDYMNGKYGSEGGPAFSGDQQPISGSWWLIGPSNDPPYGLQTDWGV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
GHYRPDQIAITDQMLFRPRK
CCCCCCEEEEEHHHHCCCCC
>Mature Secondary Structure
MDAAVPSHLVDPPAIVRDAADLRPAGRGGQLLPPGYLGTRGSQIVDVTGRPVRIASIGWN
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCC
GTEGPPGAAPSGIWKVSYRTVLDSIVAAGFNTVRIPWTDIGLDTPLNGYSDRLGWINTTL
CCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCC
NPDLLASDTPDANGRYRYVTTLVAFQRIVDYAGDIGLKVIFNHHTNQGTAGQQRNGLWFD
CCCEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEE
LGPGTDNTDGIKPGRFTAQDFKQNWLRVARTFAGNPTVIGYDLHNEPNGDRGAITWGGGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCC
PTDIKAMCEDVGSAIQDVSPDVLIICEGPETYKPPPESSGMDPRHAAPAGNLTAAGANPV
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCE
RLKIPHKLVYSIHEYPEEIADTKRWGIPETGKGFIDRMNTTWGYLVRDDIAPVWIGEMGA
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEECCCCCEEHHHCCC
SLRTPETREWARNLIDYMNGKYGSEGGPAFSGDQQPISGSWWLIGPSNDPPYGLQTDWGV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
GHYRPDQIAITDQMLFRPRK
CCCCCCEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8718854 [H]