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The map label for this gene is ilvE [H]

Identifier: 86748124

GI number: 86748124

Start: 1143283

End: 1144155

Strand: Reverse

Name: ilvE [H]

Synonym: RPB_0999

Alternate gene names: 86748124

Gene position: 1144155-1143283 (Counterclockwise)

Preceding gene: 86748125

Following gene: 86748121

Centisome position: 21.46

GC content: 66.09

Gene sequence:

>873_bases
ATGACTCCGGCCCCCGCACCGATCAGCTTCTCCAAGACCTGGACGTTCTTCGAGGGCGACTGGCACGACGGCAATGTGCC
GATCATGGGGCCGCGCACCCATGCGGCCTGGCTCGGCTCGATGGTGTTCGACGGCGCCCGCGCCTTCGAAGGCGTGACGC
CCGATCTCGACCGACACATCGCGCGGGTCAATTGGTCGGCGACCAACTTCAAGCTCAAGGCGATGATCGACGAAGCGACC
TGGCACGGCCTGGTGCAGGACGGGCTGAAGCGGTTCGCCGCCGACGCCGAACTCTACATCCGGCCGATGTATTGGGCGCA
GACCGGCGCCGGCGGCGGCGTGCTGTTCGATCCGGAGACGACCAACTGGTGCCTGTGCATCTATGAGGCGCCGATGCCGA
AGCCGACCGGGCTGTCGATCACGCTGTCGCCGTTCCGCAAGCCGAGCGCCGATTGCGCCGTGGTCGACGCCAAGGCCGGC
TGCCTCTATCCGAACAATTCGCGCGCGATGATCGAGGCGCAGAGCCGCGGGTTCGGCAACTGCCTGATGCGCGACATGCT
CGGCAACATCGCCGAGCTCGGCAATTCCAACATCTTCATGGCCAAGGACGGCGTGGTCTATACGCCGGCGCCGAACGGCA
CCTTCCTCAACGGCGTGACGCGGCAGCGCGTGATCGAGCTGCTGCGCGGCGACGGCGTCACCGTGGTCGAATCGACGCTG
ACCTATCGCGATTTCGAGACCGCGGACGAGATCTTCGCCTCGGGCAATTTCGCCAAGGTGCAGCCGATCGTCCGGATCGA
CGACCGCGCGCTGCAGCCGGGGCCGTTATTCGCCAAAGCGCGCAAGCTGTATTGGGAGTTCGCGCACGGCTGA

Upstream 100 bases:

>100_bases
GCCGCAGGCGAGCTGCGGCTGTGAGGCCGGCGCCGCACGATCAGCAAGGTTGCATAGATTTCAGTCGCGGCCCGGCCGCG
CGAAGCGATGGATCAGAACG

Downstream 100 bases:

>100_bases
GCTCGGCAAGCGTCATTCACGCGCGCTGGAAGCGTCATCCTGAGGTGCGAGCGTAGCGAGCCTCGAAGGATGCGGCGACG
GAGTGTGCGGCTCGTCCTTC

Product: aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTPAPAPISFSKTWTFFEGDWHDGNVPIMGPRTHAAWLGSMVFDGARAFEGVTPDLDRHIARVNWSATNFKLKAMIDEAT
WHGLVQDGLKRFAADAELYIRPMYWAQTGAGGGVLFDPETTNWCLCIYEAPMPKPTGLSITLSPFRKPSADCAVVDAKAG
CLYPNNSRAMIEAQSRGFGNCLMRDMLGNIAELGNSNIFMAKDGVVYTPAPNGTFLNGVTRQRVIELLRGDGVTVVESTL
TYRDFETADEIFASGNFAKVQPIVRIDDRALQPGPLFAKARKLYWEFAHG

Sequences:

>Translated_290_residues
MTPAPAPISFSKTWTFFEGDWHDGNVPIMGPRTHAAWLGSMVFDGARAFEGVTPDLDRHIARVNWSATNFKLKAMIDEAT
WHGLVQDGLKRFAADAELYIRPMYWAQTGAGGGVLFDPETTNWCLCIYEAPMPKPTGLSITLSPFRKPSADCAVVDAKAG
CLYPNNSRAMIEAQSRGFGNCLMRDMLGNIAELGNSNIFMAKDGVVYTPAPNGTFLNGVTRQRVIELLRGDGVTVVESTL
TYRDFETADEIFASGNFAKVQPIVRIDDRALQPGPLFAKARKLYWEFAHG
>Mature_289_residues
TPAPAPISFSKTWTFFEGDWHDGNVPIMGPRTHAAWLGSMVFDGARAFEGVTPDLDRHIARVNWSATNFKLKAMIDEATW
HGLVQDGLKRFAADAELYIRPMYWAQTGAGGGVLFDPETTNWCLCIYEAPMPKPTGLSITLSPFRKPSADCAVVDAKAGC
LYPNNSRAMIEAQSRGFGNCLMRDMLGNIAELGNSNIFMAKDGVVYTPAPNGTFLNGVTRQRVIELLRGDGVTVVESTLT
YRDFETADEIFASGNFAKVQPIVRIDDRALQPGPLFAKARKLYWEFAHG

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=268, Percent_Identity=27.2388059701493, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 31948; Mature: 31817

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAPAPISFSKTWTFFEGDWHDGNVPIMGPRTHAAWLGSMVFDGARAFEGVTPDLDRHI
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCCHHHHCCCCCCHHHHE
ARVNWSATNFKLKAMIDEATWHGLVQDGLKRFAADAELYIRPMYWAQTGAGGGVLFDPET
EEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCEEECCCC
TNWCLCIYEAPMPKPTGLSITLSPFRKPSADCAVVDAKAGCLYPNNSRAMIEAQSRGFGN
CCEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCEEEEECCCCCHH
CLMRDMLGNIAELGNSNIFMAKDGVVYTPAPNGTFLNGVTRQRVIELLRGDGVTVVESTL
HHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEE
TYRDFETADEIFASGNFAKVQPIVRIDDRALQPGPLFAKARKLYWEFAHG
EECCHHHHHHHHHCCCCEEECEEEEECCCCCCCCCHHHHHHHHHEEECCC
>Mature Secondary Structure 
TPAPAPISFSKTWTFFEGDWHDGNVPIMGPRTHAAWLGSMVFDGARAFEGVTPDLDRHI
CCCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCCHHHHCCCCCCHHHHE
ARVNWSATNFKLKAMIDEATWHGLVQDGLKRFAADAELYIRPMYWAQTGAGGGVLFDPET
EEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCEEECCCC
TNWCLCIYEAPMPKPTGLSITLSPFRKPSADCAVVDAKAGCLYPNNSRAMIEAQSRGFGN
CCEEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCEEEEECCCCCHH
CLMRDMLGNIAELGNSNIFMAKDGVVYTPAPNGTFLNGVTRQRVIELLRGDGVTVVESTL
HHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCEECCHHHHHHHHHHHCCCCEEEEEEE
TYRDFETADEIFASGNFAKVQPIVRIDDRALQPGPLFAKARKLYWEFAHG
EECCHHHHHHHHHCCCCEEECEEEEECCCCCCCCCHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]