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The map label for this gene is caiC [H]

Identifier: 86748125

GI number: 86748125

Start: 1144232

End: 1146100

Strand: Reverse

Name: caiC [H]

Synonym: RPB_1000

Alternate gene names: 86748125

Gene position: 1146100-1144232 (Counterclockwise)

Preceding gene: 86748129

Following gene: 86748124

Centisome position: 21.5

GC content: 69.98

Gene sequence:

>1869_bases
ATGAACATCCAACCACGATCCGTGACGGCGGACGCGACGCAGGCCCCGCCTGCGCGGGGGGCTGCGACCACTGCCCCGTC
CGTCACCAAATCTCCTGTCACCAAGCCTCCTTCCGTCACCAAGAGCTGGCTGCGGGCGATCGAGATCACCTCGCGGATCG
AGACCGAACCGCGCCGGCTGCTGGCCTCCGTGATCGACGAATGGGCCGCCGCCGCGCCGCAGCGGGACGCGATCGTGTCG
GATCGCGAATCCTTCAGCTACGCGGCGCTGGCCGATCGCATCGACCGCTACGCGCGCTGGGCGCTGACGAACGGGATCGG
GATCGGCGACGTGGTGTGTGTGCTGATGCCGAACCGGCCGGACTATCTGGCGGCGTGGCTCGGCATCACCAAGGTCGGCG
GCGTCGCGGCGCTGATCAACACCCAGCTCGTCGGCGCCTCGCTGGCGCATTGCATCGAGGTCGCGCAGCCGAAACACGTC
ATCGTCGCCGACGAACTGGCGGAGGCCTTCGCGAGCGCCCGCCCACATCTCGCGCAGGCCCCACGCGTATGGACGCATGG
CGGCGCTGGCGCCGATTCGATCGACCAGGCGCTAGCCGCGCTCGACGCCGGCCCGCTGGCGCCGCACGAACGGCGCGAGG
TCTCGATCGAGCATCTGGCGCTGCTGATCTACACCTCCGGCACCACCGGGCTGCCGAAGGCCGCGCGAGTCACGCATCGC
CGGGTGATGAGCTGGGCCGGCTGGTTCGCCGGCCTCACCGACGCCGGGCCCGGCGACCGGATGTACAATTGCCTGCCGAT
CTATCACAGCGTCGGCGGCGTGGTGGCGCCCGGCAGCCTGTTGATGGCCGGCGGCTCGGTGGTGATCGCCGAAAAGTTTT
CCGCGAGCCGGTTCTGGGACGACATCGCCCGCTGGGATTGCACGCTGTTTCAATATATCGGCGAGCTCTGCCGCTATCTG
CTGCAGGCGCCGCCGCGCGCGCGCGACACGCAGCACCGGCTGCGGCTGGCTTGCGGCAACGGGCTGCGCGGCGACGTCTG
GGAGGCGTTCCAGGCGCGCTTCGCGATTCCGCGCATCCTCGAATTCTACGCCTCGACCGAAGGCAATTTCTCGCTCTACA
ATGTCGAGGGCAGGCCCGGCGCGATCGGCCGCGTGCCGTCGTTCCTGGCGCATCGCTTTCCGGCCGCGATCGTGAAGTTC
GACCTCGACAGCGGCCTTCCGCTGCGCGGCGACGACGGGCTGTGCGTCCGCTGCGCGCGCAACGAGCCCGGCGAGGCGAT
CGGCCGGATCGGCGACGCCGCCGATCGCGGCGGCCGGTTCGAGGGCTACACCAGCGATGCCGCGAGCGACACCAAGGTGC
TGCGCGACGTGTTCGCCAGGGGCGACGCCTGGTATCGCACCGGCGACCTGATGCGGCTCGACGATCAGGGCTTCTTCCAT
TTCGTCGACCGCATCGGCGACACCTTCCGCTGGAAGGGCGAGAACGTCGCGGCGAGCGAAGTCGCCGAGGCGATCGCCGC
CTGCCCAGGCGTGACCGACGTCAGCGTCTATGGCGTCAGCGTGCCGCAGCACGACGGCCGCGCCGGCATGGCCGCGCTGG
TGGTCGACGCGCGGTTCGATATCGACGCGCTGCATCGCCATCTGGCCGATCGGCTGCCGTCCTACGCGCGCCCGCTGTTC
CTGCGGCTGCGCCCGGCGCTGGAAATCACCGGCACGTTCAAGCAGAACAAGCAGGATCTGATCCGCGACGGATTCGATCC
CGGCGTGGTGAGCGATCCGCTCTATGTCGGCGGGGCCCAGGCCGCGCGCTACGTCGCGCTCGACGAGGACCTGCACCGCC
GCATCGCCGCAGGCGAGCTGCGGCTGTGA

Upstream 100 bases:

>100_bases
TTACCTTGCGAACGCATTGTTGTCGCAACGGCGATCGATCTTCGATCGCATTCGGCTAAGTTGTATCGATAATTCTCGCA
ATGTTGTCGGACCGGCATTG

Downstream 100 bases:

>100_bases
GGCCGGCGCCGCACGATCAGCAAGGTTGCATAGATTTCAGTCGCGGCCCGGCCGCGCGAAGCGATGGATCAGAACGATGA
CTCCGGCCCCCGCACCGATC

Product: long-chain-acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNIQPRSVTADATQAPPARGAATTAPSVTKSPVTKPPSVTKSWLRAIEITSRIETEPRRLLASVIDEWAAAAPQRDAIVS
DRESFSYAALADRIDRYARWALTNGIGIGDVVCVLMPNRPDYLAAWLGITKVGGVAALINTQLVGASLAHCIEVAQPKHV
IVADELAEAFASARPHLAQAPRVWTHGGAGADSIDQALAALDAGPLAPHERREVSIEHLALLIYTSGTTGLPKAARVTHR
RVMSWAGWFAGLTDAGPGDRMYNCLPIYHSVGGVVAPGSLLMAGGSVVIAEKFSASRFWDDIARWDCTLFQYIGELCRYL
LQAPPRARDTQHRLRLACGNGLRGDVWEAFQARFAIPRILEFYASTEGNFSLYNVEGRPGAIGRVPSFLAHRFPAAIVKF
DLDSGLPLRGDDGLCVRCARNEPGEAIGRIGDAADRGGRFEGYTSDAASDTKVLRDVFARGDAWYRTGDLMRLDDQGFFH
FVDRIGDTFRWKGENVAASEVAEAIAACPGVTDVSVYGVSVPQHDGRAGMAALVVDARFDIDALHRHLADRLPSYARPLF
LRLRPALEITGTFKQNKQDLIRDGFDPGVVSDPLYVGGAQAARYVALDEDLHRRIAAGELRL

Sequences:

>Translated_622_residues
MNIQPRSVTADATQAPPARGAATTAPSVTKSPVTKPPSVTKSWLRAIEITSRIETEPRRLLASVIDEWAAAAPQRDAIVS
DRESFSYAALADRIDRYARWALTNGIGIGDVVCVLMPNRPDYLAAWLGITKVGGVAALINTQLVGASLAHCIEVAQPKHV
IVADELAEAFASARPHLAQAPRVWTHGGAGADSIDQALAALDAGPLAPHERREVSIEHLALLIYTSGTTGLPKAARVTHR
RVMSWAGWFAGLTDAGPGDRMYNCLPIYHSVGGVVAPGSLLMAGGSVVIAEKFSASRFWDDIARWDCTLFQYIGELCRYL
LQAPPRARDTQHRLRLACGNGLRGDVWEAFQARFAIPRILEFYASTEGNFSLYNVEGRPGAIGRVPSFLAHRFPAAIVKF
DLDSGLPLRGDDGLCVRCARNEPGEAIGRIGDAADRGGRFEGYTSDAASDTKVLRDVFARGDAWYRTGDLMRLDDQGFFH
FVDRIGDTFRWKGENVAASEVAEAIAACPGVTDVSVYGVSVPQHDGRAGMAALVVDARFDIDALHRHLADRLPSYARPLF
LRLRPALEITGTFKQNKQDLIRDGFDPGVVSDPLYVGGAQAARYVALDEDLHRRIAAGELRL
>Mature_622_residues
MNIQPRSVTADATQAPPARGAATTAPSVTKSPVTKPPSVTKSWLRAIEITSRIETEPRRLLASVIDEWAAAAPQRDAIVS
DRESFSYAALADRIDRYARWALTNGIGIGDVVCVLMPNRPDYLAAWLGITKVGGVAALINTQLVGASLAHCIEVAQPKHV
IVADELAEAFASARPHLAQAPRVWTHGGAGADSIDQALAALDAGPLAPHERREVSIEHLALLIYTSGTTGLPKAARVTHR
RVMSWAGWFAGLTDAGPGDRMYNCLPIYHSVGGVVAPGSLLMAGGSVVIAEKFSASRFWDDIARWDCTLFQYIGELCRYL
LQAPPRARDTQHRLRLACGNGLRGDVWEAFQARFAIPRILEFYASTEGNFSLYNVEGRPGAIGRVPSFLAHRFPAAIVKF
DLDSGLPLRGDDGLCVRCARNEPGEAIGRIGDAADRGGRFEGYTSDAASDTKVLRDVFARGDAWYRTGDLMRLDDQGFFH
FVDRIGDTFRWKGENVAASEVAEAIAACPGVTDVSVYGVSVPQHDGRAGMAALVVDARFDIDALHRHLADRLPSYARPLF
LRLRPALEITGTFKQNKQDLIRDGFDPGVVSDPLYVGGAQAARYVALDEDLHRRIAAGELRL

Specific function: Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807357, Length=586, Percent_Identity=39.7610921501706, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI227499619, Length=579, Percent_Identity=40.4145077720207, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI38524616, Length=595, Percent_Identity=38.655462184874, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI62865631, Length=593, Percent_Identity=36.5935919055649, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI13325055, Length=593, Percent_Identity=36.5935919055649, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI13236579, Length=523, Percent_Identity=40.3441682600382, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI227499621, Length=576, Percent_Identity=36.2847222222222, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI13325057, Length=595, Percent_Identity=36.8067226890756, Blast_Score=345, Evalue=5e-95,
Organism=Escherichia coli, GI221142682, Length=499, Percent_Identity=27.4549098196393, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788107, Length=590, Percent_Identity=23.0508474576271, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=24.408014571949, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115534440, Length=546, Percent_Identity=39.9267399267399, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551278, Length=590, Percent_Identity=37.1186440677966, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319515, Length=510, Percent_Identity=32.3529411764706, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=24.5125348189415, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552801, Length=608, Percent_Identity=38.6513157894737, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45550496, Length=608, Percent_Identity=38.6513157894737, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583465, Length=590, Percent_Identity=37.4576271186441, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583463, Length=590, Percent_Identity=37.4576271186441, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281364798, Length=590, Percent_Identity=37.4576271186441, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28573631, Length=575, Percent_Identity=38.2608695652174, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI20130353, Length=575, Percent_Identity=38.2608695652174, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 67407; Mature: 67407

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQPRSVTADATQAPPARGAATTAPSVTKSPVTKPPSVTKSWLRAIEITSRIETEPRRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
LASVIDEWAAAAPQRDAIVSDRESFSYAALADRIDRYARWALTNGIGIGDVVCVLMPNRP
HHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
DYLAAWLGITKVGGVAALINTQLVGASLAHCIEVAQPKHVIVADELAEAFASARPHLAQA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCHHCC
PRVWTHGGAGADSIDQALAALDAGPLAPHERREVSIEHLALLIYTSGTTGLPKAARVTHR
CCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHEEEEEEECCCCCCCHHHHHHHH
RVMSWAGWFAGLTDAGPGDRMYNCLPIYHSVGGVVAPGSLLMAGGSVVIAEKFSASRFWD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEECCCCEEEECCCEEEEECCCHHHHHH
DIARWDCTLFQYIGELCRYLLQAPPRARDTQHRLRLACGNGLRGDVWEAFQARFAIPRIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EFYASTEGNFSLYNVEGRPGAIGRVPSFLAHRFPAAIVKFDLDSGLPLRGDDGLCVRCAR
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECC
NEPGEAIGRIGDAADRGGRFEGYTSDAASDTKVLRDVFARGDAWYRTGDLMRLDDQGFFH
CCCHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCEEEECCCHHHH
FVDRIGDTFRWKGENVAASEVAEAIAACPGVTDVSVYGVSVPQHDGRAGMAALVVDARFD
HHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCC
IDALHRHLADRLPSYARPLFLRLRPALEITGTFKQNKQDLIRDGFDPGVVSDPLYVGGAQ
HHHHHHHHHHHCCHHHCCCEEEECCCEEEEECHHCCHHHHHHCCCCCCCCCCCCEECCHH
AARYVALDEDLHRRIAAGELRL
HHEEEEECHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIQPRSVTADATQAPPARGAATTAPSVTKSPVTKPPSVTKSWLRAIEITSRIETEPRRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHH
LASVIDEWAAAAPQRDAIVSDRESFSYAALADRIDRYARWALTNGIGIGDVVCVLMPNRP
HHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
DYLAAWLGITKVGGVAALINTQLVGASLAHCIEVAQPKHVIVADELAEAFASARPHLAQA
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCHHCC
PRVWTHGGAGADSIDQALAALDAGPLAPHERREVSIEHLALLIYTSGTTGLPKAARVTHR
CCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHEEEEEEECCCCCCCHHHHHHHH
RVMSWAGWFAGLTDAGPGDRMYNCLPIYHSVGGVVAPGSLLMAGGSVVIAEKFSASRFWD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEECCCCEEEECCCEEEEECCCHHHHHH
DIARWDCTLFQYIGELCRYLLQAPPRARDTQHRLRLACGNGLRGDVWEAFQARFAIPRIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EFYASTEGNFSLYNVEGRPGAIGRVPSFLAHRFPAAIVKFDLDSGLPLRGDDGLCVRCAR
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEECC
NEPGEAIGRIGDAADRGGRFEGYTSDAASDTKVLRDVFARGDAWYRTGDLMRLDDQGFFH
CCCHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCEEEECCCHHHH
FVDRIGDTFRWKGENVAASEVAEAIAACPGVTDVSVYGVSVPQHDGRAGMAALVVDARFD
HHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCC
IDALHRHLADRLPSYARPLFLRLRPALEITGTFKQNKQDLIRDGFDPGVVSDPLYVGGAQ
HHHHHHHHHHHCCHHHCCCEEEECCCEEEEECHHCCHHHHHHCCCCCCCCCCCCEECCHH
AARYVALDEDLHRRIAAGELRL
HHEEEEECHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA