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The map label for this gene is menE [H]

Identifier: 86748120

GI number: 86748120

Start: 1138773

End: 1140155

Strand: Reverse

Name: menE [H]

Synonym: RPB_0995

Alternate gene names: 86748120

Gene position: 1140155-1138773 (Counterclockwise)

Preceding gene: 86748121

Following gene: 86748119

Centisome position: 21.38

GC content: 70.07

Gene sequence:

>1383_bases
ATGTCCCCGCGTGAACTCGTTGCGCTGCGCAACTATCTCGCAGCCGGCCTGAGCGACCGCACGCTCTCGGACGCCAGCGA
CAGCGTCGCGATGGCGGAGGTCGCGCGCGGCACCGCGCTCGGCGGACGGCTGCCCGAATTGGCGGGCCGCTGCGTGCTGC
TGTCGCTGGCGACGCAGCTGATCAGCGGCATCGCGATGATCGAGATCGACGGCGTCGCGCGCCGGATGCTGCTGTGCCCG
CCCGATTTCGACATGAAATACGCGCCCGACCTGATCGCCGACGCGCAGATCGACGCCGTCGTCACCGACGATCCGGCGCG
CTGGCAAAGCTTGGGCATCGAACTGATCGTCACCGCCGGCCTGCCGCTGCGGCCGGCCAAGCCGATCGAACTCACGCACG
CCACCCAATGGTTGATGCTGACCTCCGGCACCACCGGCGCGCCGAAGATCGTGGCGCATACGCTGGCGGCGCTGACCGGC
GCGATCGTCGCCGATGGGACCGCACCGGGCGCGCCGCCGACCTGGGCGACGTTCTACGACATCCGCCGCTATGGCGGGCT
GCAGATCTTCCTCCGCGCCGTGCTCGGCGGCGGCTCGATGGTGCTGTCCTCGCCCGGCGAAGCGCTCGTCGATCATCTGG
CGCGGCTCGCCGCGCGCGGCGTCACGCATATTTCCGGCACGCCGTCGCATTGGCGCAAGGCGCTGATGAGCGCCGCGCTG
GCGTCGTTCAGTCCGGACTACGTCCGACTGTCCGGCGAGATCGCCGATCAGGCGGTGCTCGACAGCCTGCGCCGCGCCTT
CCCGAGCGCCTCGATCGGCCACGCTTACGCCTCCACCGAGGCCGGCGTCGGCTTCGCCGTCAATGACGGCCGCGAAGGTT
TCCCCGCGTCGATGATCGGCGCCGGCCCCAATGGCGTCGACATGCAGGTGATCGACGGCTCGCTGCGGATCCGCTCCGGA
CGCACCGCGCACACTTATGTCGGGCGCAGCGCGCCGGCGCTGACCGACGCCGACGGCTTCGTCGACACCGGCGATATGGT
CGAACTGCGCGACGATCGTTACTACTTCGTCGGCCGGCGCGACGGCGTCATCAATGTCGGCGGCCTCAAGGTGCATCCCG
AAGAGGTCGAAGCCGTGATCAACCGCCATCCCGCGGTGCGGATGTCGCGGATTCGCGCGCGGCGCAGCCCGATCACCGGC
GCGCTGGTGGTCGCCGACGTGATGCTGGCGAGCGTCTCCGATTCCGGCCGCACCGGCGAGATCCGGGAGGCGATCCTCGC
CGATTGCAAGGCGTCGCTGCCGCCCCACAAGGTCCCGGCGATGGTGAACTTCGTCCAGCAGCTCGACGTCACCGCCTCCG
GCAAGCTGGCCCGCCATGCGTAA

Upstream 100 bases:

>100_bases
GTGGCGCGGCTGGAAGACAGCCTCGGCGTCGATCCGTTCACCGCCGCCGACGACGCGGTCTTTCCGGTGACGCTGGGCGA
TTTCATCGAGGCGTACGAGA

Downstream 100 bases:

>100_bases
GGTGGTGGTCACCGGCGGCAGCCGCGGCATCGGGCTGGCGATCGCGCAGCGGCTCGCCGCCGCCGGCTACGGCGTCATCG
CCGTGGCGCGGCGCGAGAGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSPRELVALRNYLAAGLSDRTLSDASDSVAMAEVARGTALGGRLPELAGRCVLLSLATQLISGIAMIEIDGVARRMLLCP
PDFDMKYAPDLIADAQIDAVVTDDPARWQSLGIELIVTAGLPLRPAKPIELTHATQWLMLTSGTTGAPKIVAHTLAALTG
AIVADGTAPGAPPTWATFYDIRRYGGLQIFLRAVLGGGSMVLSSPGEALVDHLARLAARGVTHISGTPSHWRKALMSAAL
ASFSPDYVRLSGEIADQAVLDSLRRAFPSASIGHAYASTEAGVGFAVNDGREGFPASMIGAGPNGVDMQVIDGSLRIRSG
RTAHTYVGRSAPALTDADGFVDTGDMVELRDDRYYFVGRRDGVINVGGLKVHPEEVEAVINRHPAVRMSRIRARRSPITG
ALVVADVMLASVSDSGRTGEIREAILADCKASLPPHKVPAMVNFVQQLDVTASGKLARHA

Sequences:

>Translated_460_residues
MSPRELVALRNYLAAGLSDRTLSDASDSVAMAEVARGTALGGRLPELAGRCVLLSLATQLISGIAMIEIDGVARRMLLCP
PDFDMKYAPDLIADAQIDAVVTDDPARWQSLGIELIVTAGLPLRPAKPIELTHATQWLMLTSGTTGAPKIVAHTLAALTG
AIVADGTAPGAPPTWATFYDIRRYGGLQIFLRAVLGGGSMVLSSPGEALVDHLARLAARGVTHISGTPSHWRKALMSAAL
ASFSPDYVRLSGEIADQAVLDSLRRAFPSASIGHAYASTEAGVGFAVNDGREGFPASMIGAGPNGVDMQVIDGSLRIRSG
RTAHTYVGRSAPALTDADGFVDTGDMVELRDDRYYFVGRRDGVINVGGLKVHPEEVEAVINRHPAVRMSRIRARRSPITG
ALVVADVMLASVSDSGRTGEIREAILADCKASLPPHKVPAMVNFVQQLDVTASGKLARHA
>Mature_459_residues
SPRELVALRNYLAAGLSDRTLSDASDSVAMAEVARGTALGGRLPELAGRCVLLSLATQLISGIAMIEIDGVARRMLLCPP
DFDMKYAPDLIADAQIDAVVTDDPARWQSLGIELIVTAGLPLRPAKPIELTHATQWLMLTSGTTGAPKIVAHTLAALTGA
IVADGTAPGAPPTWATFYDIRRYGGLQIFLRAVLGGGSMVLSSPGEALVDHLARLAARGVTHISGTPSHWRKALMSAALA
SFSPDYVRLSGEIADQAVLDSLRRAFPSASIGHAYASTEAGVGFAVNDGREGFPASMIGAGPNGVDMQVIDGSLRIRSGR
TAHTYVGRSAPALTDADGFVDTGDMVELRDDRYYFVGRRDGVINVGGLKVHPEEVEAVINRHPAVRMSRIRARRSPITGA
LVVADVMLASVSDSGRTGEIREAILADCKASLPPHKVPAMVNFVQQLDVTASGKLARHA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI58082049, Length=346, Percent_Identity=25.1445086705202, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=348, Percent_Identity=27.5862068965517, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI38505220, Length=353, Percent_Identity=26.0623229461756, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI157311624, Length=346, Percent_Identity=25.4335260115607, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI157311622, Length=346, Percent_Identity=25.4335260115607, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI42544132, Length=350, Percent_Identity=26, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI115511026, Length=356, Percent_Identity=23.876404494382, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788595, Length=273, Percent_Identity=27.1062271062271, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 48492; Mature: 48361

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRELVALRNYLAAGLSDRTLSDASDSVAMAEVARGTALGGRLPELAGRCVLLSLATQL
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ISGIAMIEIDGVARRMLLCPPDFDMKYAPDLIADAQIDAVVTDDPARWQSLGIELIVTAG
HCCEEEEEECCHHHHEEECCCCCCCCCCCCHHCCCCEEEEECCCCHHHHHCCEEEEEECC
LPLRPAKPIELTHATQWLMLTSGTTGAPKIVAHTLAALTGAIVADGTAPGAPPTWATFYD
CCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
IRRYGGLQIFLRAVLGGGSMVLSSPGEALVDHLARLAARGVTHISGTPSHWRKALMSAAL
HHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ASFSPDYVRLSGEIADQAVLDSLRRAFPSASIGHAYASTEAGVGFAVNDGREGFPASMIG
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEECCCCCCCCCHHCC
AGPNGVDMQVIDGSLRIRSGRTAHTYVGRSAPALTDADGFVDTGDMVELRDDRYYFVGRR
CCCCCCEEEEECCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECC
DGVINVGGLKVHPEEVEAVINRHPAVRMSRIRARRSPITGALVVADVMLASVSDSGRTGE
CCEEEECCEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCH
IREAILADCKASLPPHKVPAMVNFVQQLDVTASGKLARHA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure 
SPRELVALRNYLAAGLSDRTLSDASDSVAMAEVARGTALGGRLPELAGRCVLLSLATQL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ISGIAMIEIDGVARRMLLCPPDFDMKYAPDLIADAQIDAVVTDDPARWQSLGIELIVTAG
HCCEEEEEECCHHHHEEECCCCCCCCCCCCHHCCCCEEEEECCCCHHHHHCCEEEEEECC
LPLRPAKPIELTHATQWLMLTSGTTGAPKIVAHTLAALTGAIVADGTAPGAPPTWATFYD
CCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
IRRYGGLQIFLRAVLGGGSMVLSSPGEALVDHLARLAARGVTHISGTPSHWRKALMSAAL
HHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ASFSPDYVRLSGEIADQAVLDSLRRAFPSASIGHAYASTEAGVGFAVNDGREGFPASMIG
HHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEEECCCCCCCCCHHCC
AGPNGVDMQVIDGSLRIRSGRTAHTYVGRSAPALTDADGFVDTGDMVELRDDRYYFVGRR
CCCCCCEEEEECCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECC
DGVINVGGLKVHPEEVEAVINRHPAVRMSRIRARRSPITGALVVADVMLASVSDSGRTGE
CCEEEECCEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCH
IREAILADCKASLPPHKVPAMVNFVQQLDVTASGKLARHA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA