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The map label for this gene is fixA [H]

Identifier: 86748109

GI number: 86748109

Start: 1129448

End: 1130344

Strand: Direct

Name: fixA [H]

Synonym: RPB_0984

Alternate gene names: 86748109

Gene position: 1129448-1130344 (Clockwise)

Preceding gene: 86748108

Following gene: 86748110

Centisome position: 21.18

GC content: 66.89

Gene sequence:

>897_bases
ATGTCCCTGTGTCAGGCTGATGTCGTCGTGTCAGCAAGCGCGGAGCACACCATGCATATCGTCGTCTGTATCAAGCAGGT
CCCGGACTCGGCGCAGATCCGCGTGCATCCGGTCACCAACACGATCATGCGCCAGGGCGTGCCGACGATCATCAACCCGT
ATGATCTGTTCGCGCTCGAAGAGGCGCTGCGGCTGCGCGACAAGTTCGGCGGCGAGGTCACGGTGCTGACGATGGGCCCG
CCCTCGGCCGAGGATTCCCTGCGCAAGGCGCTGACCTTCGGCGCCAACCGCGCGGTGCTGCTGACCGACCGCTTCTTCGC
CGGCGCCGATACGCTGGCGACCAGCTACGCGCTGGCTTCCGCGATCCGCAAGATCGGCGCCACCTATGGCGAACCGGACA
TCGTGTTCACCGGCAAGCAGACCATCGACGGCGATACCGCCCAGGTCGGCCCCGGCATCTGCAAGCGGCTCGACTTCCTG
CAGCTCACTTACGTCTCCAAGGTCGCGTCGCTGGATCTCGCCGGCCGCAGCATCGAGGTCGAGCGCCGCTCCGAGGGCGG
CGTCCAGGTGCTGCGCAGCCGGCTGCCTTGCCTGATCACCATGCTGGAGGGCACCAACGAGGTGCGTCGCGGCACGATGC
TGAATGCGCTGAACGCCGCCCGCGCCGAAGTGGTGAAGTGGAGCGCCCAGGACGCCGGCGTCGAAGACATTTCCAAATGC
GGCCTGCGCGGCTCGCCGACCGTGGTCAAGCGCGTGTTCGCGCCGACCGCGCGTTCGGAAAAAGCCGTGGTGGTGGAGCC
GGCCGGCCGCCCGCCGGCCGACGCGCTGATCGACGAGATCTTCAAGCTCCGGCCGAAACTCGAAGCCGACATGTTCGAGC
TCGCGCGTGGATTTTGA

Upstream 100 bases:

>100_bases
CATTCATCGAGAACCATTCCAAGGCGATCGCCGGTCCGACCCAAGCCGTTGCTTTTTCGATCGAATTTCATCGGCCGAAA
AGTTGGTACGAGGCTTGCTG

Downstream 100 bases:

>100_bases
CAACACCAATTTGAAAGCAGTCTCATGAGCCAGCCAGCAAAGCCTGCCCCGCCCGCCGGACGCGCCAACGCCAAGAAGGA
GCTGTCCGAGCACTTCAAGC

Product: electron transfer flavoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLCQADVVVSASAEHTMHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKFGGEVTVLTMGP
PSAEDSLRKALTFGANRAVLLTDRFFAGADTLATSYALASAIRKIGATYGEPDIVFTGKQTIDGDTAQVGPGICKRLDFL
QLTYVSKVASLDLAGRSIEVERRSEGGVQVLRSRLPCLITMLEGTNEVRRGTMLNALNAARAEVVKWSAQDAGVEDISKC
GLRGSPTVVKRVFAPTARSEKAVVVEPAGRPPADALIDEIFKLRPKLEADMFELARGF

Sequences:

>Translated_298_residues
MSLCQADVVVSASAEHTMHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKFGGEVTVLTMGP
PSAEDSLRKALTFGANRAVLLTDRFFAGADTLATSYALASAIRKIGATYGEPDIVFTGKQTIDGDTAQVGPGICKRLDFL
QLTYVSKVASLDLAGRSIEVERRSEGGVQVLRSRLPCLITMLEGTNEVRRGTMLNALNAARAEVVKWSAQDAGVEDISKC
GLRGSPTVVKRVFAPTARSEKAVVVEPAGRPPADALIDEIFKLRPKLEADMFELARGF
>Mature_297_residues
SLCQADVVVSASAEHTMHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEEALRLRDKFGGEVTVLTMGPP
SAEDSLRKALTFGANRAVLLTDRFFAGADTLATSYALASAIRKIGATYGEPDIVFTGKQTIDGDTAQVGPGICKRLDFLQ
LTYVSKVASLDLAGRSIEVERRSEGGVQVLRSRLPCLITMLEGTNEVRRGTMLNALNAARAEVVKWSAQDAGVEDISKCG
LRGSPTVVKRVFAPTARSEKAVVVEPAGRPPADALIDEIFKLRPKLEADMFELARGF

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI4503609, Length=224, Percent_Identity=33.4821428571429, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI62420877, Length=211, Percent_Identity=33.6492890995261, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI2367123, Length=216, Percent_Identity=30.0925925925926, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081682, Length=269, Percent_Identity=26.7657992565056, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25141345, Length=222, Percent_Identity=34.2342342342342, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321646, Length=192, Percent_Identity=31.25, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651147, Length=223, Percent_Identity=31.390134529148, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24651145, Length=223, Percent_Identity=31.390134529148, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 32184; Mature: 32052

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLCQADVVVSASAEHTMHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALE
CCCCCCCEEEECCCCCEEEEEEEEECCCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHH
EALRLRDKFGGEVTVLTMGPPSAEDSLRKALTFGANRAVLLTDRFFAGADTLATSYALAS
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCHHCCHHHHHHHHHHHH
AIRKIGATYGEPDIVFTGKQTIDGDTAQVGPGICKRLDFLQLTYVSKVASLDLAGRSIEV
HHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ERRSEGGVQVLRSRLPCLITMLEGTNEVRRGTMLNALNAARAEVVKWSAQDAGVEDISKC
EECCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHC
GLRGSPTVVKRVFAPTARSEKAVVVEPAGRPPADALIDEIFKLRPKLEADMFELARGF
CCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SLCQADVVVSASAEHTMHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALE
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCEEEEECHHHHHHCCCCCEECCHHHHHHH
EALRLRDKFGGEVTVLTMGPPSAEDSLRKALTFGANRAVLLTDRFFAGADTLATSYALAS
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCHHCCHHHHHHHHHHHH
AIRKIGATYGEPDIVFTGKQTIDGDTAQVGPGICKRLDFLQLTYVSKVASLDLAGRSIEV
HHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ERRSEGGVQVLRSRLPCLITMLEGTNEVRRGTMLNALNAARAEVVKWSAQDAGVEDISKC
EECCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHC
GLRGSPTVVKRVFAPTARSEKAVVVEPAGRPPADALIDEIFKLRPKLEADMFELARGF
CCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088; 3216855 [H]