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The map label for this gene is nifU [H]

Identifier: 86748105

GI number: 86748105

Start: 1125207

End: 1126208

Strand: Direct

Name: nifU [H]

Synonym: RPB_0980

Alternate gene names: 86748105

Gene position: 1125207-1126208 (Clockwise)

Preceding gene: 86748104

Following gene: 86748106

Centisome position: 21.1

GC content: 69.36

Gene sequence:

>1002_bases
ATGCTCGGCAATCTCGACAGGCTCGATGAACTCGTCTCCAGTCCGCGCAATGCCGGCGTGCTGCTGCAGGCCAACGCGAT
CGGCGCCTTCGGCGGCATCCGCTGGGGCGATGCGGTCAAGCTGATGCTGCGGGTCGATCCGGCGACCGACCGGATCGAGC
AGGCGCGGTTTCAGGCGTTCGGCTGCAGCTCGTCGATCGCCGCGGCGTCCGCGGTCACCGAACTGATCACCGGCAAGACG
CTGGACGACGCCGCGGCGCTCGGCGCGGCCGACATCGCCGACGATCTCGGCGGCTTGCCGGCGGCGCGGATGTATTGCGC
GGTGATGGCCTACGAGGCGCTACAGACGGCGATCACGTCCTATCGGGGCATTGCGGCGCTGCGCGAGGCCGACGCGGCGC
CGTCGTGCAAGTGCCTCGGCGTCAGCCAGATGATGATCGAGCGCACCATCCGCTTCAATCGCCTGACCAGCCTGGAACAG
GTGACCCACTACACCAAGGCGGCCGGGAGCTGCAGCTCCTGCTTCAAGCAGGTCGAAGGCCTGCTGGCGCGGGTTAATGC
CGAGATGGTCGAGGACGGGCTGATCGACGCTGGCGCGGCGTATCAGCTCGGCTCGACGCAGCAGCGGGCCGTCGACCTGA
AGCCGCACGGCGCGCCGCAGCCGGCGGCCAACATCTTCTCCGGCAAAGCGGCGCCGGCGCATCTGCGCGCGATGCCGAAG
AGCCCGCCGCCGCGTCCGGCGACGGCGCAGGCGCCGGTCGCCGGGACCATCGACGCGCTGCCGCTGACTTCTCTGGTGGC
CGAAGCGCTGGAGGATTTGCGGCCGCATCTGCAGCGCGACGGCGGCGATTGCGAACTCGTCAGCGTCGAGGGCAATGTCG
TCTATGTCCGGCTGTCGGGCAATTGCGTCGGCTGCCAATTGTCATCGGTGACGCTGTCCGGCGTCCAGGCCAGACTCGCC
GACAAGCTCGGCCGGCCGCTGCGCGTGGTGCCGGTGTCATGA

Upstream 100 bases:

>100_bases
CTCGAAGGCTCCGGCTTCACCTTCGACAACCCGAACGCCGAGAACAAATGCGGCTGCGGCAAGTCGTTCGGCTGAGTGCC
TCAAGCCACGGAGCGTCGTC

Downstream 100 bases:

>100_bases
GCCAGACCCGCACCCCGCCACAGCCGACCAACATCGTCTATCTCGACGCCAACGCCACCACGCGCACCGATCCGCGCGTG
GTCGATGCGATGCTGCCGTT

Product: nitrogen-fixing NifU-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLGNLDRLDELVSSPRNAGVLLQANAIGAFGGIRWGDAVKLMLRVDPATDRIEQARFQAFGCSSSIAAASAVTELITGKT
LDDAAALGAADIADDLGGLPAARMYCAVMAYEALQTAITSYRGIAALREADAAPSCKCLGVSQMMIERTIRFNRLTSLEQ
VTHYTKAAGSCSSCFKQVEGLLARVNAEMVEDGLIDAGAAYQLGSTQQRAVDLKPHGAPQPAANIFSGKAAPAHLRAMPK
SPPPRPATAQAPVAGTIDALPLTSLVAEALEDLRPHLQRDGGDCELVSVEGNVVYVRLSGNCVGCQLSSVTLSGVQARLA
DKLGRPLRVVPVS

Sequences:

>Translated_333_residues
MLGNLDRLDELVSSPRNAGVLLQANAIGAFGGIRWGDAVKLMLRVDPATDRIEQARFQAFGCSSSIAAASAVTELITGKT
LDDAAALGAADIADDLGGLPAARMYCAVMAYEALQTAITSYRGIAALREADAAPSCKCLGVSQMMIERTIRFNRLTSLEQ
VTHYTKAAGSCSSCFKQVEGLLARVNAEMVEDGLIDAGAAYQLGSTQQRAVDLKPHGAPQPAANIFSGKAAPAHLRAMPK
SPPPRPATAQAPVAGTIDALPLTSLVAEALEDLRPHLQRDGGDCELVSVEGNVVYVRLSGNCVGCQLSSVTLSGVQARLA
DKLGRPLRVVPVS
>Mature_333_residues
MLGNLDRLDELVSSPRNAGVLLQANAIGAFGGIRWGDAVKLMLRVDPATDRIEQARFQAFGCSSSIAAASAVTELITGKT
LDDAAALGAADIADDLGGLPAARMYCAVMAYEALQTAITSYRGIAALREADAAPSCKCLGVSQMMIERTIRFNRLTSLEQ
VTHYTKAAGSCSSCFKQVEGLLARVNAEMVEDGLIDAGAAYQLGSTQQRAVDLKPHGAPQPAANIFSGKAAPAHLRAMPK
SPPPRPATAQAPVAGTIDALPLTSLVAEALEDLRPHLQRDGGDCELVSVEGNVVYVRLSGNCVGCQLSSVTLSGVQARLA
DKLGRPLRVVPVS

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI24307953, Length=112, Percent_Identity=36.6071428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56699456, Length=112, Percent_Identity=36.6071428571429, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788878, Length=112, Percent_Identity=38.3928571428571, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543474, Length=120, Percent_Identity=35.8333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325122, Length=119, Percent_Identity=37.8151260504202, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324800, Length=113, Percent_Identity=35.3982300884956, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355597, Length=116, Percent_Identity=36.2068965517241, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 34780; Mature: 34780

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGNLDRLDELVSSPRNAGVLLQANAIGAFGGIRWGDAVKLMLRVDPATDRIEQARFQAF
CCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GCSSSIAAASAVTELITGKTLDDAAALGAADIADDLGGLPAARMYCAVMAYEALQTAITS
CCCCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YRGIAALREADAAPSCKCLGVSQMMIERTIRFNRLTSLEQVTHYTKAAGSCSSCFKQVEG
HHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLARVNAEMVEDGLIDAGAAYQLGSTQQRAVDLKPHGAPQPAANIFSGKAAPAHLRAMPK
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEECCCCCCCCCHHHHHCCCCCCHHHHCCCC
SPPPRPATAQAPVAGTIDALPLTSLVAEALEDLRPHLQRDGGDCELVSVEGNVVYVRLSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECC
NCVGCQLSSVTLSGVQARLADKLGRPLRVVPVS
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MLGNLDRLDELVSSPRNAGVLLQANAIGAFGGIRWGDAVKLMLRVDPATDRIEQARFQAF
CCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GCSSSIAAASAVTELITGKTLDDAAALGAADIADDLGGLPAARMYCAVMAYEALQTAITS
CCCCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YRGIAALREADAAPSCKCLGVSQMMIERTIRFNRLTSLEQVTHYTKAAGSCSSCFKQVEG
HHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LLARVNAEMVEDGLIDAGAAYQLGSTQQRAVDLKPHGAPQPAANIFSGKAAPAHLRAMPK
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEECCCCCCCCCHHHHHCCCCCCHHHHCCCC
SPPPRPATAQAPVAGTIDALPLTSLVAEALEDLRPHLQRDGGDCELVSVEGNVVYVRLSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECC
NCVGCQLSSVTLSGVQARLADKLGRPLRVVPVS
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9503607 [H]