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The map label for this gene is nifS [H]

Identifier: 86748106

GI number: 86748106

Start: 1126205

End: 1127428

Strand: Direct

Name: nifS [H]

Synonym: RPB_0981

Alternate gene names: 86748106

Gene position: 1126205-1127428 (Clockwise)

Preceding gene: 86748105

Following gene: 86748107

Centisome position: 21.12

GC content: 69.04

Gene sequence:

>1224_bases
ATGAGCCAGACCCGCACCCCGCCACAGCCGACCAACATCGTCTATCTCGACGCCAACGCCACCACGCGCACCGATCCGCG
CGTGGTCGATGCGATGCTGCCGTTCTTCTCCGGCTATTTCGGCAATCCGTCGTCCAAGCATGCGCTCGGCGGCCATGCGG
CGCGTGCGGTGAAGCACGCCCGCGAGCAGTTGCAGGCGCTGGTCGGCGCCGCGCATCCGCACGAGCTGATCTTCACCTCC
GGCGGCACCGAGAGCGCCAACACCGCGATCCTGTCGGCGCTGGAGGCCGCGCCGCGGCGCCGCGAGATCATCACCACCGC
GGTCGAGCATCCCGCCGTGCTGTCGCTGTGCGCCTGGCTGGAAAAGAACAAGGGCATCCGCGTTCACGTCATCCCGGTCG
ATCGCCAGGGCCATCTCGACATCGCGGCCTATCGCGACGCGCTGTCCGACCGCGTCGCGCTGGTGTCGATGATGTGGGCC
AACAACGAGACCGGCGTGATCAACCCGGTCGCCGACCTCGCCGAACTGTCGAAAGAGGTCGGCGCGCTGTTTCATACCGA
CGCGGTGCAGGCGGTCGGCAAATGCCCGATCGAACTGCAATCCACCGCGATCGACATGCTGTCGGTGTCCGGCCACAAGC
TGCACGGCCCGAAGGGCATCGGCGCGCTGTATGTCCGCAGCGGCGCGGCCTTCAAGCCGCAGATCAAGGGCGGCCAGCAC
GAGCGCGGCCGCCGCGCCGGCACCGAGAACGTGCCCGGCATCGTCGGCCTCGGCATGGCGGCCGAACTCGCCGCGGGCGC
GATGGCCGACGAGGACATCCGGGTGCGCGGCTTGCGCGACCGGCTGGAGCACGAGATCCTGGCGCGCGTCGATCATTGCG
TCGCCATCGGCGCCCGCGCCGAGCGGCTGCCGAACACCTCGAACATCGCGTTCTCGTATATCGACAGCGAAGCCATCGTC
ACTTTGCTCGACCGCGCCGGCATCGCCGCCTCGATGGGCTCGGCCTGTGCGACAGGCTCGTTCGAGCCGTCGCATGTGCT
GATGGCGATGAAGGTCGCCGAGGACAGCGTGCGCGGCGGCGTGCGGTTCTCGCTGTCGCGCGACAACACCGACGACGACA
TCGAGCGCGCGCTCGCCGTCATCCCCGGCGTGATCGCCAAGCTGCGCGCGATCTCGCCGTTCACCGCCGATGACGGACCT
TTGCTCGGGCGCGCCCATGCGTGA

Upstream 100 bases:

>100_bases
GCAATTGCGTCGGCTGCCAATTGTCATCGGTGACGCTGTCCGGCGTCCAGGCCAGACTCGCCGACAAGCTCGGCCGGCCG
CTGCGCGTGGTGCCGGTGTC

Downstream 100 bases:

>100_bases
CAGCAGCGCAACGCCATCGACCGCGACGCCGGTCGTGCTCAACGACACCACGCTGCGCGACGGCGAGCAGGCGCCGGGCG
TGGCGTTCTCGGCCGCCGAA

Product: aminotransferase, class V

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSQTRTPPQPTNIVYLDANATTRTDPRVVDAMLPFFSGYFGNPSSKHALGGHAARAVKHAREQLQALVGAAHPHELIFTS
GGTESANTAILSALEAAPRRREIITTAVEHPAVLSLCAWLEKNKGIRVHVIPVDRQGHLDIAAYRDALSDRVALVSMMWA
NNETGVINPVADLAELSKEVGALFHTDAVQAVGKCPIELQSTAIDMLSVSGHKLHGPKGIGALYVRSGAAFKPQIKGGQH
ERGRRAGTENVPGIVGLGMAAELAAGAMADEDIRVRGLRDRLEHEILARVDHCVAIGARAERLPNTSNIAFSYIDSEAIV
TLLDRAGIAASMGSACATGSFEPSHVLMAMKVAEDSVRGGVRFSLSRDNTDDDIERALAVIPGVIAKLRAISPFTADDGP
LLGRAHA

Sequences:

>Translated_407_residues
MSQTRTPPQPTNIVYLDANATTRTDPRVVDAMLPFFSGYFGNPSSKHALGGHAARAVKHAREQLQALVGAAHPHELIFTS
GGTESANTAILSALEAAPRRREIITTAVEHPAVLSLCAWLEKNKGIRVHVIPVDRQGHLDIAAYRDALSDRVALVSMMWA
NNETGVINPVADLAELSKEVGALFHTDAVQAVGKCPIELQSTAIDMLSVSGHKLHGPKGIGALYVRSGAAFKPQIKGGQH
ERGRRAGTENVPGIVGLGMAAELAAGAMADEDIRVRGLRDRLEHEILARVDHCVAIGARAERLPNTSNIAFSYIDSEAIV
TLLDRAGIAASMGSACATGSFEPSHVLMAMKVAEDSVRGGVRFSLSRDNTDDDIERALAVIPGVIAKLRAISPFTADDGP
LLGRAHA
>Mature_406_residues
SQTRTPPQPTNIVYLDANATTRTDPRVVDAMLPFFSGYFGNPSSKHALGGHAARAVKHAREQLQALVGAAHPHELIFTSG
GTESANTAILSALEAAPRRREIITTAVEHPAVLSLCAWLEKNKGIRVHVIPVDRQGHLDIAAYRDALSDRVALVSMMWAN
NETGVINPVADLAELSKEVGALFHTDAVQAVGKCPIELQSTAIDMLSVSGHKLHGPKGIGALYVRSGAAFKPQIKGGQHE
RGRRAGTENVPGIVGLGMAAELAAGAMADEDIRVRGLRDRLEHEILARVDHCVAIGARAERLPNTSNIAFSYIDSEAIVT
LLDRAGIAASMGSACATGSFEPSHVLMAMKVAEDSVRGGVRFSLSRDNTDDDIERALAVIPGVIAKLRAISPFTADDGPL
LGRAHA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=39.0745501285347, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=32.6923076923077, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=41.6020671834625, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789175, Length=391, Percent_Identity=30.1790281329923, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787970, Length=387, Percent_Identity=27.390180878553, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=392, Percent_Identity=40.3061224489796, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17533177, Length=311, Percent_Identity=33.4405144694534, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=392, Percent_Identity=37.7551020408163, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=40.7792207792208, Blast_Score=277, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43184; Mature: 43053

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTRTPPQPTNIVYLDANATTRTDPRVVDAMLPFFSGYFGNPSSKHALGGHAARAVKHA
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
REQLQALVGAAHPHELIFTSGGTESANTAILSALEAAPRRREIITTAVEHPAVLSLCAWL
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
EKNKGIRVHVIPVDRQGHLDIAAYRDALSDRVALVSMMWANNETGVINPVADLAELSKEV
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GALFHTDAVQAVGKCPIELQSTAIDMLSVSGHKLHGPKGIGALYVRSGAAFKPQIKGGQH
HHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCC
ERGRRAGTENVPGIVGLGMAAELAAGAMADEDIRVRGLRDRLEHEILARVDHCVAIGARA
HHHCCCCCCCCCCCEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERLPNTSNIAFSYIDSEAIVTLLDRAGIAASMGSACATGSFEPSHVLMAMKVAEDSVRGG
HHCCCCCCEEEEEECHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHEEHHHHHHHHHCCC
VRFSLSRDNTDDDIERALAVIPGVIAKLRAISPFTADDGPLLGRAHA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQTRTPPQPTNIVYLDANATTRTDPRVVDAMLPFFSGYFGNPSSKHALGGHAARAVKHA
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
REQLQALVGAAHPHELIFTSGGTESANTAILSALEAAPRRREIITTAVEHPAVLSLCAWL
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHH
EKNKGIRVHVIPVDRQGHLDIAAYRDALSDRVALVSMMWANNETGVINPVADLAELSKEV
HCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GALFHTDAVQAVGKCPIELQSTAIDMLSVSGHKLHGPKGIGALYVRSGAAFKPQIKGGQH
HHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCC
ERGRRAGTENVPGIVGLGMAAELAAGAMADEDIRVRGLRDRLEHEILARVDHCVAIGARA
HHHCCCCCCCCCCCEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERLPNTSNIAFSYIDSEAIVTLLDRAGIAASMGSACATGSFEPSHVLMAMKVAEDSVRGG
HHCCCCCCEEEEEECHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHEEHHHHHHHHHCCC
VRFSLSRDNTDDDIERALAVIPGVIAKLRAISPFTADDGPLLGRAHA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]