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The map label for this gene is nifN [H]

Identifier: 86748098

GI number: 86748098

Start: 1120829

End: 1122205

Strand: Direct

Name: nifN [H]

Synonym: RPB_0973

Alternate gene names: 86748098

Gene position: 1120829-1122205 (Clockwise)

Preceding gene: 86748097

Following gene: 86748099

Centisome position: 21.02

GC content: 68.55

Gene sequence:

>1377_bases
ATGGCCAGGATCGTGACATCGACCAAGTCCTGCACCGTCAATCCGCTGCGGATGAGTCGGCCGCTCGGCGCGGCGCTGGC
CTTGATGGGGCTGCGCAATGCGATGCCGCTGCTGCACGGCTCGCAGGGCTGCACCTCGTTCGGCCTGGTGCTGTTCGTGC
GGCATTTCCGCGAACAGATCCCGATGCAGACCACCGCGATGAGCGAAGTCGCCACCGTGCTCGGCGGCTTCGAGAATGTC
GAGCAGGCGATCGTCAACATCGTCGGCCGCACCCAGCCGGACGTGATCGGGATCTGCACCACGGGCGTCACCGAGATCAA
GGGCGACGACCTCGACGGCTTCATCAAGGACGTCCGCCGCAAGCATCCCGAACTCGCGCATGTCGCGCTGGTGCCGGTGT
CGACGCCGGACTTCAAGGGCGCGTTCGAGGACGGCTTCGCCAGCACCGTGGCGAAGATCGTCGAGCTGCTGGTCGAGGCG
CCAGCGCCGGGCGCCGCGCGCGATCCGGCGCGGCTCAACGTGCTGGCCGGCAGCCATCTGACGCCGGGCGATATCGACGA
GCTGCGCGACGTCATCGAGGCGTTCGGCCTGGTGCCGACCTTCCTGCCGGACATTTCCGGCTCGCTCGACGGCCATCTGC
CGGACGACTTCACCCCGACCACCCATGGCGGCGTCTCGGTCCCCGAGGTCGCGGCGATGGGCGGCGCGGCGCATACGCTG
GCGCTCGGCGAGCAGATGCGCAAGGCCGCGGCCGCGCTCGAGGCCAAGGCCGGCGTGCCGTTCACGCTGCTGCGGCGGCT
CACCGGGCTCGCGGCCGGCGACGAACTGATGGCGACGCTGGCCAAGATCAGCGGCCGGCCGGTGCCGCCGAAATATCGCC
GGCAGCGCAGCCAGCTGGTCGACGCCATGCTCGACGGCCACTTCTATTTCGGCGGCAAGCAGGTCGCGATCGGCGCCGAG
CCGGACATGCTGCTGAATATCGGCGGCTGGCTCGCCGACATGGGCTGCACGATCGAGGCTGCGGTAACGACGACCAACTC
GCAGGCGCTTTCGCAGGTGCCGGCCGACGAGGTGCTGATCGGCGATCTGGAAGATCTGGAGAGCCGCGCCGAGGAGTGCG
ATCTGCTGCTGACGCATTCGCACGGCAGGCAAGCCGCCGAGCGGCTCGGCGTGCCGCTGTTCCGCGTCGGCATTCCGATG
TTCGATCGGCTCGGCGCCGCGCATCAGGTCGTGGTCGGCTATCGCGGCAGCCGCGATCTGATCTTTGCGATCGGCAATCT
GTTCATCGCCGCCATCAAGGAACCGCATGTCGACGACTGGCGCAACGCCGCGATCGGCGATCGGGATCAGGTCGATGCGG
CGGCTACGGCTCATTAG

Upstream 100 bases:

>100_bases
TGGGACGAAGTGAGCTGGGAGACCCGCGCCGACGCCGCCAATGCCGCCGACGACGCACAGCGCGCCGCCGAGGCCGCACC
CGCCGCAAAGGTCGCGTGAC

Downstream 100 bases:

>100_bases
GACGGACGCGAAGGAGACTGTCGATGCACAGCAACGGAGGCGCGGCATGAAGGTCGCTTTTGCCACTCAGGATCTGACGC
GCGTCGACGCGCATTTCGGC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MARIVTSTKSCTVNPLRMSRPLGAALALMGLRNAMPLLHGSQGCTSFGLVLFVRHFREQIPMQTTAMSEVATVLGGFENV
EQAIVNIVGRTQPDVIGICTTGVTEIKGDDLDGFIKDVRRKHPELAHVALVPVSTPDFKGAFEDGFASTVAKIVELLVEA
PAPGAARDPARLNVLAGSHLTPGDIDELRDVIEAFGLVPTFLPDISGSLDGHLPDDFTPTTHGGVSVPEVAAMGGAAHTL
ALGEQMRKAAAALEAKAGVPFTLLRRLTGLAAGDELMATLAKISGRPVPPKYRRQRSQLVDAMLDGHFYFGGKQVAIGAE
PDMLLNIGGWLADMGCTIEAAVTTTNSQALSQVPADEVLIGDLEDLESRAEECDLLLTHSHGRQAAERLGVPLFRVGIPM
FDRLGAAHQVVVGYRGSRDLIFAIGNLFIAAIKEPHVDDWRNAAIGDRDQVDAAATAH

Sequences:

>Translated_458_residues
MARIVTSTKSCTVNPLRMSRPLGAALALMGLRNAMPLLHGSQGCTSFGLVLFVRHFREQIPMQTTAMSEVATVLGGFENV
EQAIVNIVGRTQPDVIGICTTGVTEIKGDDLDGFIKDVRRKHPELAHVALVPVSTPDFKGAFEDGFASTVAKIVELLVEA
PAPGAARDPARLNVLAGSHLTPGDIDELRDVIEAFGLVPTFLPDISGSLDGHLPDDFTPTTHGGVSVPEVAAMGGAAHTL
ALGEQMRKAAAALEAKAGVPFTLLRRLTGLAAGDELMATLAKISGRPVPPKYRRQRSQLVDAMLDGHFYFGGKQVAIGAE
PDMLLNIGGWLADMGCTIEAAVTTTNSQALSQVPADEVLIGDLEDLESRAEECDLLLTHSHGRQAAERLGVPLFRVGIPM
FDRLGAAHQVVVGYRGSRDLIFAIGNLFIAAIKEPHVDDWRNAAIGDRDQVDAAATAH
>Mature_457_residues
ARIVTSTKSCTVNPLRMSRPLGAALALMGLRNAMPLLHGSQGCTSFGLVLFVRHFREQIPMQTTAMSEVATVLGGFENVE
QAIVNIVGRTQPDVIGICTTGVTEIKGDDLDGFIKDVRRKHPELAHVALVPVSTPDFKGAFEDGFASTVAKIVELLVEAP
APGAARDPARLNVLAGSHLTPGDIDELRDVIEAFGLVPTFLPDISGSLDGHLPDDFTPTTHGGVSVPEVAAMGGAAHTLA
LGEQMRKAAAALEAKAGVPFTLLRRLTGLAAGDELMATLAKISGRPVPPKYRRQRSQLVDAMLDGHFYFGGKQVAIGAEP
DMLLNIGGWLADMGCTIEAAVTTTNSQALSQVPADEVLIGDLEDLESRAEECDLLLTHSHGRQAAERLGVPLFRVGIPMF
DRLGAAHQVVVGYRGSRDLIFAIGNLFIAAIKEPHVDDWRNAAIGDRDQVDAAATAH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 48613; Mature: 48482

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVTSTKSCTVNPLRMSRPLGAALALMGLRNAMPLLHGSQGCTSFGLVLFVRHFREQI
CCCEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGFENVEQAIVNIVGRTQPDVIGICTTGVTEIKGDDLDGFIKDVRR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPELAHVALVPVSTPDFKGAFEDGFASTVAKIVELLVEAPAPGAARDPARLNVLAGSHL
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
TPGDIDELRDVIEAFGLVPTFLPDISGSLDGHLPDDFTPTTHGGVSVPEVAAMGGAAHTL
CCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
ALGEQMRKAAAALEAKAGVPFTLLRRLTGLAAGDELMATLAKISGRPVPPKYRRQRSQLV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
DAMLDGHFYFGGKQVAIGAEPDMLLNIGGWLADMGCTIEAAVTTTNSQALSQVPADEVLI
HHHHCCEEEECCEEEEECCCCCEEEECCHHHHHCCCEEEEEEEECCHHHHHHCCCCCEEE
GDLEDLESRAEECDLLLTHSHGRQAAERLGVPLFRVGIPMFDRLGAAHQVVVGYRGSRDL
CCHHHHHHHHHHCCEEEECCCCHHHHHHHCCCHHHHCCCHHHHCCCCEEEEEEECCCCHH
IFAIGNLFIAAIKEPHVDDWRNAAIGDRDQVDAAATAH
HHHHHHHHHHHHCCCCCCCHHCCCCCCCHHCCHHHCCC
>Mature Secondary Structure 
ARIVTSTKSCTVNPLRMSRPLGAALALMGLRNAMPLLHGSQGCTSFGLVLFVRHFREQI
CCEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGFENVEQAIVNIVGRTQPDVIGICTTGVTEIKGDDLDGFIKDVRR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPELAHVALVPVSTPDFKGAFEDGFASTVAKIVELLVEAPAPGAARDPARLNVLAGSHL
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
TPGDIDELRDVIEAFGLVPTFLPDISGSLDGHLPDDFTPTTHGGVSVPEVAAMGGAAHTL
CCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHH
ALGEQMRKAAAALEAKAGVPFTLLRRLTGLAAGDELMATLAKISGRPVPPKYRRQRSQLV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
DAMLDGHFYFGGKQVAIGAEPDMLLNIGGWLADMGCTIEAAVTTTNSQALSQVPADEVLI
HHHHCCEEEECCEEEEECCCCCEEEECCHHHHHCCCEEEEEEEECCHHHHHHCCCCCEEE
GDLEDLESRAEECDLLLTHSHGRQAAERLGVPLFRVGIPMFDRLGAAHQVVVGYRGSRDL
CCHHHHHHHHHHCCEEEECCCCHHHHHHHCCCHHHHCCCHHHHCCCCEEEEEEECCCCHH
IFAIGNLFIAAIKEPHVDDWRNAAIGDRDQVDAAATAH
HHHHHHHHHHHHCCCCCCCHHCCCCCCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]