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The map label for this gene is nifE [H]

Identifier: 86748097

GI number: 86748097

Start: 1119361

End: 1120827

Strand: Direct

Name: nifE [H]

Synonym: RPB_0972

Alternate gene names: 86748097

Gene position: 1119361-1120827 (Clockwise)

Preceding gene: 86748096

Following gene: 86748098

Centisome position: 20.99

GC content: 65.1

Gene sequence:

>1467_bases
ATGAGCCGTCTCGCAGACAAGATCCAAGATGTCTTCAACGAGCCCGGCTGTGCGGACAATCAGGCCAAGTCCGAGAAGCA
ACGCAAGAAGGGCTGCAGCAAACCGCTGCAGCCCGGCGGCGCGGCCGGCGGCTGCGCCTTCGACGGCGCCAAGATCGCGC
TGCAGCCGATCGTCGACGTCGCCCATCTGGTGCACGGCCCGATCGCCTGCGAAGGCTCGTCCTGGGACAATCGCGGCACC
AAATCGTCGGGCTCGAAGCTGTATCGCACCGGCTTCACCACCGACATGAGCGAGAACGACGTGGTGTTCGGCGGCGAGAA
GCGGCTGTTCCGGTCGATCAGGGAAATCATCGAGAAGTACGACCCGCCCGCGGTGTTCGTGTATCAGACCTGCGTGCCGG
CGATGATGGGCGACGACATCGTCGCGGTCTGTAAGGTGGCGGCCGAGAAATTCGGCAAGCCCTGCATCCCGATCATCGCG
CCGGGCTTCGTCGGCCCGAAGAATCTCGGCAACAAGCTCGCCGGCGAGGCGATGCTCGACTACGTGATCGGCACGCAGGA
GCCGGAGGTCACCACCCCCTACGACATCAACATCATCGGCGAATACAACGTCGCCGGCGAATTGTGGCAGGTCAAGCCGC
TGCTCGACGAGCTCGGCATCCGCATCCTGTCCTGCCTGTCGGGCGACGCGCGCTATCACGAGGTCGCGCAGTCGCATCGC
GCCCGCGCCGCCATGATGGTGTGCTCGACCGCGATGATCAACGTCGCCCGCAAGATGCAGGAGCGCTACGGCATTCCGTA
TTTCGAGGGCTCGTTCTACGGCATCACCGACACCTCGGATTCGCTGCGGCAGATCGCACGGCTGCTGATCGCGCGCGGCG
CCGATGCCGAGCTGATGGACCGCGTCGAGGCGCTGATCGCGCGCGAGGAGGCCAAGGCCTGGGCCGCCATCAAGGCCTAT
ACGCCGCGGCTCGCAGGCAAGAAAGTGCTGCTGATCACCGGCGGCGTGAAGTCGTGGTCGGTGGTGCTGGCGCTGCAGGA
AGCCGGGCTCACCATCGTCGGCACCAGCGTCAAGAAATCGACCAAGGAGGACAAGGAGCGGCTCAAGGAGATGAGTCCCG
ACGTCCATCTGATCGACGATCTGCGGCCGCGCGAAATGTACAAGATGCTGAAAGAGGCGCAGGCCGACATAATGTTGTCC
GGCGGCCGCTCGCAATTCGTCGCGCTGAAGGCGCGGATGCCCTGGATGGATATCAACCAGGAACGCTCTTACGCGTATTG
CGGCTATGTCGGCATTGTCGAGATGGTGCGGCAGATCGACAAGGCGCTGTCGAACCCGATCTGGCAGCAGGTCCGCTCGG
CGCCGCCGTGGGACGAAGTGAGCTGGGAGACCCGCGCCGACGCCGCCAATGCCGCCGACGACGCACAGCGCGCCGCCGAG
GCCGCACCCGCCGCAAAGGTCGCGTGA

Upstream 100 bases:

>100_bases
CACCAACATCGCCGGCAAGACCGACCTGTCCTTCGACATCATCCGCTGATGCCGAAGGCGATACCAAAGTCTCGGACTAC
CTGATCCTCAGGAGCGTCGC

Downstream 100 bases:

>100_bases
CATGGCCAGGATCGTGACATCGACCAAGTCCTGCACCGTCAATCCGCTGCGGATGAGTCGGCCGCTCGGCGCGGCGCTGG
CCTTGATGGGGCTGCGCAAT

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSRLADKIQDVFNEPGCADNQAKSEKQRKKGCSKPLQPGGAAGGCAFDGAKIALQPIVDVAHLVHGPIACEGSSWDNRGT
KSSGSKLYRTGFTTDMSENDVVFGGEKRLFRSIREIIEKYDPPAVFVYQTCVPAMMGDDIVAVCKVAAEKFGKPCIPIIA
PGFVGPKNLGNKLAGEAMLDYVIGTQEPEVTTPYDINIIGEYNVAGELWQVKPLLDELGIRILSCLSGDARYHEVAQSHR
ARAAMMVCSTAMINVARKMQERYGIPYFEGSFYGITDTSDSLRQIARLLIARGADAELMDRVEALIAREEAKAWAAIKAY
TPRLAGKKVLLITGGVKSWSVVLALQEAGLTIVGTSVKKSTKEDKERLKEMSPDVHLIDDLRPREMYKMLKEAQADIMLS
GGRSQFVALKARMPWMDINQERSYAYCGYVGIVEMVRQIDKALSNPIWQQVRSAPPWDEVSWETRADAANAADDAQRAAE
AAPAAKVA

Sequences:

>Translated_488_residues
MSRLADKIQDVFNEPGCADNQAKSEKQRKKGCSKPLQPGGAAGGCAFDGAKIALQPIVDVAHLVHGPIACEGSSWDNRGT
KSSGSKLYRTGFTTDMSENDVVFGGEKRLFRSIREIIEKYDPPAVFVYQTCVPAMMGDDIVAVCKVAAEKFGKPCIPIIA
PGFVGPKNLGNKLAGEAMLDYVIGTQEPEVTTPYDINIIGEYNVAGELWQVKPLLDELGIRILSCLSGDARYHEVAQSHR
ARAAMMVCSTAMINVARKMQERYGIPYFEGSFYGITDTSDSLRQIARLLIARGADAELMDRVEALIAREEAKAWAAIKAY
TPRLAGKKVLLITGGVKSWSVVLALQEAGLTIVGTSVKKSTKEDKERLKEMSPDVHLIDDLRPREMYKMLKEAQADIMLS
GGRSQFVALKARMPWMDINQERSYAYCGYVGIVEMVRQIDKALSNPIWQQVRSAPPWDEVSWETRADAANAADDAQRAAE
AAPAAKVA
>Mature_487_residues
SRLADKIQDVFNEPGCADNQAKSEKQRKKGCSKPLQPGGAAGGCAFDGAKIALQPIVDVAHLVHGPIACEGSSWDNRGTK
SSGSKLYRTGFTTDMSENDVVFGGEKRLFRSIREIIEKYDPPAVFVYQTCVPAMMGDDIVAVCKVAAEKFGKPCIPIIAP
GFVGPKNLGNKLAGEAMLDYVIGTQEPEVTTPYDINIIGEYNVAGELWQVKPLLDELGIRILSCLSGDARYHEVAQSHRA
RAAMMVCSTAMINVARKMQERYGIPYFEGSFYGITDTSDSLRQIARLLIARGADAELMDRVEALIAREEAKAWAAIKAYT
PRLAGKKVLLITGGVKSWSVVLALQEAGLTIVGTSVKKSTKEDKERLKEMSPDVHLIDDLRPREMYKMLKEAQADIMLSG
GRSQFVALKARMPWMDINQERSYAYCGYVGIVEMVRQIDKALSNPIWQQVRSAPPWDEVSWETRADAANAADDAQRAAEA
APAAKVA

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 53415; Mature: 53284

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLADKIQDVFNEPGCADNQAKSEKQRKKGCSKPLQPGGAAGGCAFDGAKIALQPIVDV
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHGPIACEGSSWDNRGTKSSGSKLYRTGFTTDMSENDVVFGGEKRLFRSIREIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMMGDDIVAVCKVAAEKFGKPCIPIIAPGFVGPKNLGNKLAGEAMLD
CCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHH
YVIGTQEPEVTTPYDINIIGEYNVAGELWQVKPLLDELGIRILSCLSGDARYHEVAQSHR
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARAAMMVCSTAMINVARKMQERYGIPYFEGSFYGITDTSDSLRQIARLLIARGADAELMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHH
RVEALIAREEAKAWAAIKAYTPRLAGKKVLLITGGVKSWSVVLALQEAGLTIVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHCCEEEEECCHHHH
TKEDKERLKEMSPDVHLIDDLRPREMYKMLKEAQADIMLSGGRSQFVALKARMPWMDINQ
HHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCCC
ERSYAYCGYVGIVEMVRQIDKALSNPIWQQVRSAPPWDEVSWETRADAANAADDAQRAAE
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHH
AAPAAKVA
HCCCCCCC
>Mature Secondary Structure 
SRLADKIQDVFNEPGCADNQAKSEKQRKKGCSKPLQPGGAAGGCAFDGAKIALQPIVDV
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHGPIACEGSSWDNRGTKSSGSKLYRTGFTTDMSENDVVFGGEKRLFRSIREIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMMGDDIVAVCKVAAEKFGKPCIPIIAPGFVGPKNLGNKLAGEAMLD
CCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHH
YVIGTQEPEVTTPYDINIIGEYNVAGELWQVKPLLDELGIRILSCLSGDARYHEVAQSHR
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARAAMMVCSTAMINVARKMQERYGIPYFEGSFYGITDTSDSLRQIARLLIARGADAELMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHH
RVEALIAREEAKAWAAIKAYTPRLAGKKVLLITGGVKSWSVVLALQEAGLTIVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEHHCCEEEEECCHHHH
TKEDKERLKEMSPDVHLIDDLRPREMYKMLKEAQADIMLSGGRSQFVALKARMPWMDINQ
HHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCCC
ERSYAYCGYVGIVEMVRQIDKALSNPIWQQVRSAPPWDEVSWETRADAANAADDAQRAAE
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHH
AAPAAKVA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]