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The map label for this gene is nifH [H]

Identifier: 86748094

GI number: 86748094

Start: 1115228

End: 1116127

Strand: Direct

Name: nifH [H]

Synonym: RPB_0969

Alternate gene names: 86748094

Gene position: 1115228-1116127 (Clockwise)

Preceding gene: 86748093

Following gene: 86748095

Centisome position: 20.92

GC content: 65.22

Gene sequence:

>900_bases
ATGGCGGCACTTCGGCAAATCGCATTCTACGGCAAGGGCGGCATCGGCAAGTCGACCACTTCGCAGAACACCCTCGCGGC
TCTGGTCGAGCTCGGTCAGAAGATCCTGATCGTCGGCTGCGACCCCAAGGCGGACTCCACCCGCCTGATCCTCAACACCA
AGATGCAGGACACCGTGCTCAGCCTCGCCGCTGAAGCGGGTTCGGTCGAAGACCTCGAACTCGAAGACGTGATGAAGATC
GGCTACAAGGGCATCAAGTGCACCGAAGCCGGCGGCCCGGAGCCGGGCGTCGGCTGCGCCGGCCGCGGCGTCATCACCGC
GATCAACTTCCTCGAAGAGAACGGCGCCTATGAGGACGTCGACTACGTCTCCTACGACGTGCTCGGCGACGTGGTCTGCG
GCGGCTTCGCGATGCCGATCCGCGAGAACAAGGCCCAGGAAATCTACATCGTGATGTCCGGCGAGATGATGGCGCTGTAC
GCCGCCAACAACATCGCCAAGGGCATTCTGAAGTACGCCTCCTCGGGCGGCGTCCGCCTCGGCGGCCTGATCTGCAACGA
GCGCCAGACCGACCGCGAGCTCGACCTCGCCGAAGCGCTGGCGGCCCGGCTGAACTCCAAGCTGATCCACTTCGTGCCGC
GCGCCAACATCGTGCAGCACGCCGAGCTGCGCCGCCAGACCGTGATCGAATACGCGCCCGACTCGCAGCAGGCGCAGGAA
TATCGCCAGCTCGCCAACAAGATCCACGCCAACTCCGGCAACGGCACCATCCCGACCCCGATCACGATGGAAGAGCTGGA
AGGCATGCTGCTCGACTTCGGCATCATGAAGACCGACGAGCAGGCGCTGGCCGAGCTCGCCGAGAAGGAAGCCGCCAAGG
CGGCCGCCGCCACCGCCTGA

Upstream 100 bases:

>100_bases
AAGGGCAACATCCGCTTCTGGGCCGTGCGACGGATTAACGGAATGAGGCTGCGCGGTTCGCGCACATCGATCAACGGCTC
GAGAACAACGGAGAGAAAAC

Downstream 100 bases:

>100_bases
TCCATCAGCCGGGCCGGCCGCCTTGCGCGGCCGGCTCCCCGGCGGCCCCTTACACGAGGAACAACGATGAGCACCGCAGT
CGCAGAATCCCCCGCGGACA

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAALRQIAFYGKGGIGKSTTSQNTLAALVELGQKILIVGCDPKADSTRLILNTKMQDTVLSLAAEAGSVEDLELEDVMKI
GYKGIKCTEAGGPEPGVGCAGRGVITAINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYASSGGVRLGGLICNERQTDRELDLAEALAARLNSKLIHFVPRANIVQHAELRRQTVIEYAPDSQQAQE
YRQLANKIHANSGNGTIPTPITMEELEGMLLDFGIMKTDEQALAELAEKEAAKAAAATA

Sequences:

>Translated_299_residues
MAALRQIAFYGKGGIGKSTTSQNTLAALVELGQKILIVGCDPKADSTRLILNTKMQDTVLSLAAEAGSVEDLELEDVMKI
GYKGIKCTEAGGPEPGVGCAGRGVITAINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYASSGGVRLGGLICNERQTDRELDLAEALAARLNSKLIHFVPRANIVQHAELRRQTVIEYAPDSQQAQE
YRQLANKIHANSGNGTIPTPITMEELEGMLLDFGIMKTDEQALAELAEKEAAKAAAATA
>Mature_298_residues
AALRQIAFYGKGGIGKSTTSQNTLAALVELGQKILIVGCDPKADSTRLILNTKMQDTVLSLAAEAGSVEDLELEDVMKIG
YKGIKCTEAGGPEPGVGCAGRGVITAINFLEENGAYEDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
ANNIAKGILKYASSGGVRLGGLICNERQTDRELDLAEALAARLNSKLIHFVPRANIVQHAELRRQTVIEYAPDSQQAQEY
RQLANKIHANSGNGTIPTPITMEELEGMLLDFGIMKTDEQALAELAEKEAAKAAAATA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31960; Mature: 31829

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALRQIAFYGKGGIGKSTTSQNTLAALVELGQKILIVGCDPKADSTRLILNTKMQDTVL
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHH
SLAAEAGSVEDLELEDVMKIGYKGIKCTEAGGPEPGVGCAGRGVITAINFLEENGAYEDV
HHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYASSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCEEE
GGLICNERQTDRELDLAEALAARLNSKLIHFVPRANIVQHAELRRQTVIEYAPDSQQAQE
EEEEECCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHH
YRQLANKIHANSGNGTIPTPITMEELEGMLLDFGIMKTDEQALAELAEKEAAKAAAATA
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AALRQIAFYGKGGIGKSTTSQNTLAALVELGQKILIVGCDPKADSTRLILNTKMQDTVL
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHH
SLAAEAGSVEDLELEDVMKIGYKGIKCTEAGGPEPGVGCAGRGVITAINFLEENGAYEDV
HHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYASSGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCEEE
GGLICNERQTDRELDLAEALAARLNSKLIHFVPRANIVQHAELRRQTVIEYAPDSQQAQE
EEEEECCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHH
YRQLANKIHANSGNGTIPTPITMEELEGMLLDFGIMKTDEQALAELAEKEAAKAAAATA
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2744485; 9163424 [H]